限制酶切分析 SGT1引物所扩出的片段.doc

限制性酶切分析 SGT1引物所扩出的片段 甲基化：dam-否 dcm-否使用 117 酶屏蔽，找到39 个位点AccIII T/CCGGA 1: 190AflII C/TTAAG 1: 481(宝生目录A-10)ApaI GGGCC/C 1: 1270AsuII TT/CGAA 1: 183BalI TGG/CCA 1: 1164BbvII GAAGACNN/ 2: 1478, 1513BclI T/GATCA 2: 1176, 1316BglII A/GATCT 1: 160Bsc91I GAAGACNN/ 2: 1478, 1513BsmI GAATGCN/ 2: 849, 1392Bsp1407I T/GTACA 2: 437, 1037BspHI T/CATGA 2: 678, 1097BspMI ACCTGCNNNN/ 2: 19, 711BspMII T/CCGGA 1: 190BstD102I GAG/CGG 1: 762BstXI CCANNNNN/NTGG 1: 746Csp45I TT/CGAA 1: 183Eco57I CTGAAGNNNNNNNNNNNNNNNN/ 2: 254, 269HpaI GTT/AAC 1: 84MfeI C/AATTG 1: 1301Mlu113I CC/GCGG 1: 109MscI TGG/CCA 1: 1164NdeI CA/TATG 1: 708PflMI CCANNNN/NTGG 1: 933RleAI CCCACANNNNNNNNNNNN/ 1: 1073SacII CCGC/GG 1: 111SspI AAT/ATT 1: 1347SstII CCGC/GG 1: 111StuI AGG/CCT 1: 972XbaI T/CTAGA 1: 513(宝生目录A-32)XcmI CCANNNNN/NNNNTGG 1: 1157按位点顺序列出19 BspMI 254 Eco57I 849 BsmI 1270 ApaI84 HpaI 269 Eco57I 933 PflMI 1301 MfeI109 Mlu113I 437 Bsp1407I 972 StuI 1316 BclI111 SstII 481 AflII 1037 Bsp1407I 1347 SspI111 SacII 513 XbaI 1073 RleAI 1392 BsmI160 BglII 678 BspHI 1097 BspHI 1478 Bsc91I183 AsuII 708 NdeI 1157 XcmI 1478 BbvII183 Csp45I 711 BspMI 1164 BalI 1513 BbvII190 BspMII 746 BstXI 1164 MscI 1513 Bsc91I190 AccIII 762 BstD102I 1176 BclI 未被下列酶剪切AatII Acc65I AclI AgeI AhaIII Alw44IAlwNI ApaBI ApaLI AscI Asp718I AvrIIBamHI BbeI BglI Bpu1102I BsiI BssHIIBstEII Bsu36I ClaI CspI CvnI DraIDraIII DrdI EagI Eam1105I Ecl136II Eco31IEco47III Eco52I Eco56I Eco72I EcoICRI EcoNIEcoRI EcoRV EheI EspI FseI HindIIII-PpoI KpnI MluI MstI MstII NaeINarI NcoI NheI NotI NruI NsiIPacI PinAI PmaCI PmeI PstI PvuIPvuII SacI SalI SapI SauI ScaISciI SfiI SgrAI SmaI SnaBI SpeISphI SplI SpoI SrfI SstI SunISwaI Tth111I VspI XhoI XmaI XmaIIIXmnI XorII Restriction sites on SGT1引物所扩出的片段 BspMI1 ATGGTAGCAACCTGCAACAATGGCGAAATCCTCCATGTTCTTTTCCTTCCCTTCTTATCC TACCATCGTTGGACGTTGTTACCGCTTTAGGAGGTACAAGAAAAGGAAGGGAAGAATAGG SacII SstII HpaI Mlu113I61 GCTGGTCATTTCATCCCATTAGTTAACGCCGCAAGGCTATTCGCCTCCCGCGGTGTTAAA CGACCAGTAAAGTAGGGTAATCAATTGCGGCGTTCCGATAAGCGGAGGGCGCCACAATTT BglII121 GCCACAATCCTCACTACCCCTCATAATGCCTTACTTTTTAGATCTACTATTGACGATGAT CGGTGTTAGGAGTGATGGGGAGTATTACGGAATGAAAAATCTAGATGATAACTGCTACTA Csp45I AccIII AsuII BspMII181 GTTCGAATTTCCGGATTTCCCATTTCTATCGTAACTATTAAATTCCCCTCTGCTGAAGTT CAAGCTTAAAGGCCTAAAGGGTAAAGATAGCATTGATAATTTAAGGGGAGACGACTTCAA Eco57I Eco57I241 GGGTTGCCTGAAGGAATTGAGAGCTTTAACTCTGCCACTTCACCTGAAATGCCTCATAAA CCCAACGGACTTCCTTAACTCTCGAAATTGAGACGGTGAAGTGGACTTTACGGAGTATTT301 ATTTTTTATGCTCTTTCTCTTCTACAAAAGCCAATGGAAGATAAAATTCGTGAACTCCGT TAAAAAATACGAGAAAGAGAAGATGTTTTCGGTTACCTTCTATTTTAAGCACTTGAGGCA361 CCTGATTGCATTTTTTCTGATATGTACTTCCCTTGGACAGTAGATATTGCTGATGAGCTT GGACTAACGTAAAAAAGACTATACATGAAGGGAACCTGTCATCTATAACGACTACTCGAA Bsp1407I421 CACATCCCTCGTATTTTGTACAATTTGTCTGCTTACATGTGCTACAGCATTATGCACAAC GTGTAGGGAGCATAAAACATGTTAAACAGACGAATGTACACGATGTCGTAATACGTGTTG AflII XbaI（宝生A-32）481 CTTAAGGTTTACAGACCTCACAAGCAGCCTAATCTAGACGAATCTCAAAGTTTCGTGGTT GAATTCCAAATGTCTGGAGTGTTCGTCGGATTAGATCTGCTTAGAGTTTCAAAGCACCAA541 CCTGGTTTACCTGATGAGATAAAGTTCAAGTTATCCCAACTGACAGATGATCTGAGAAAG GGACCAAATGGACTACTCTATTTCAAGTTCAATAGGGTTGACTGTCTACTAGACTCTTTC601 TCGGATGACCAAAAGACTGTTTTTGACGAATTGCTCGAACAAGTTGAAGATTCGGAGGAA AGCCTACTGGTTTTCTGACAAAAACTGCTTAACGAGCTTGTTCAACTTCTAAGCCTCCTT BspMI BspHI NdeI661 CGAAGCTATGGCATTGTTCATGATACATTTTATGAGCTAGAACCTGCATATGTTGACTAC GCTTCGATACCGTAACAAGTACTATGTAAAATACTCGATCTTGGACGTATACAACTGATG BstXI BstD102I721 TACCAGAAATTAAAGAAACCAAAATGTTGGCATTTTGGTCCGCTCTCTCATTTTGCATCC ATGGTCTTTAATTTCTTTGGTTTTACAACCGTAAAACCAGGCGAGAGAGTAAAACGTAGG781 AAAATCCGTAGTAAGGAACTAATTTCTGAGCATAACAACAATGAGATTGTTATAGATTGG TTTTAGGCATCATTCCTTGATTAAAGACTCGTATTGTTGTTACTCTAACAATATCTAACC BsmI841 TTGAATGCACAGAAACCTAAATCGGTTCTCTATGTATCTTTCGGAAGCATGGCTAGATTT AACTTACGTGTCTTTGGATTTAGCCAAGAGATACATAGAAAGCCTTCGTACCGATCTAAA PflMI901 CCTGAGAGCCAACTGAATGAAATAGCCCAAGCTCTGGATGCTTCAAATGTTCCTTTCATT GGACTCTCGGTTGACTTACTTTATCGGGTTCGAGACCTACGAAGTTTACAAGGAAAGTAA StuI961 TTTGTATTGAGGCCTAATGAAGAAACGGCGTCGTGGTTGCCAGTTGGTAATTTAGAGGAC AAACATAACTCCGGATTACTTCTTTGCCGCAGCACCAACGGTCAACCATTAAATCTCCTG Bsp1407I RleAI1021 AAGACTAAAAAGGGTTTGTACATCAAAGGGTGGGTCCCACAGCTTACGATCATGGAACAT TTCTGATTTTTCCCAAACATGTAGTTTCCCACCCAGGGTGTCGAATGCTAGTACCTTGTA BspHI1081 TCAGCAACAGGCGGGTTCATGACTCATTGTGGTACTAATTCGGTTCTGGAAGCCATCACT AGTCGTTGTCCGCCCAAGTACTGAGTAACACCATGATTAAGCCAAGACCTTCGGTAGTGA MscI XcmI BalI BclI1141 TTTGGCGTGCCAATGATAACATGGCCACTTTATGCTGATCAATTCTACAACGAGAAGGTA AAACCGCACGGTTACTATTGTACCGGTGAAATACGACTAGTTAAGATGTTGCTCTTCCAT1201 GTCGAGGTTAGGGGATTGGGAATCAAAATCGGGATAGATGTATGGAATGAAGGGATTGAG CAGCTCCAATCCCCTAACCCTTAGTTTTAGCCCTATCTACATACCTTACTTCCCTAACTC ApaI MfeI BclI1261 ATCACGGGCCCTGTAATAGAAAGCGCCAAGATTAGAGAAGCAATTGAGAGACTAATGATC TAGTGCCCGGGACATTATCTTTCGCGGTTCTAATCTCTTCGTTAACTCTCTGATTACTAG SspI1321 AGTAATGGTTCTGAGGAAATTATAAATATTAGGGATAGAGTAATGGCTATGAGCAAAATG TCATTACCAAGACTCCTTTAATATTTATAATCCCTATCTCATTACCGATACTCGTTTTAC BsmI1381 GCTCAGAATGCAACAAATGAAGGTGGATCTTCGTGGAACAATCTCACTGCTCTCATTCAA CGAGTCTTACGTTGTTTACTTCCACCTAGAA