激发波长和发射滤光片的选择.doc

Syngene provides full flexibility throughout the range of imaging sysems, with excitation energy sources available for all fluorescent, colorimetric and chemifluorescent applications. The chart below lists the dye together with the excitation and emission wavelengths. The Filter column suggests which of the Syngene filter types is the best choice for that application. For further information, please see Technical Note 10 (under Technical Support/Application Notes).Dye/Stain Excitation range (nm) Peaks (nm) Excitation source Emission range (nm) Peaks (nm) FilterChemiluminescence n/a n/a n/a Substrate dependent 425, 430, 440, 461, 560 noneChemifluorescence 350-500 440 Blue 500-650560 SG Coomassie Blue White light n/a White lightvisible blue n/a noneFilm White light n/a White lightvisible brown n/a noneSilver White light n/a White light visible orange/brown n/a noneEthidium Bromide 250-600 302 Mid UV 550-700 595 UV Fluorescein/FITC 220-330 290 Mid UV 480-620 518 SP 425-525494Blue480-620518SGwt GFP 300-500 395 Mid or Long UV 480-580 508 SP 470 Blue480-580 508 SG eGFP/rsGFP 350-525 488 Blue470-600 508 SG Hoechst 33258 300-400 348 Mid or Long UV 380-650 450 SP Alexa 488 425-540 495 Blue480-625 520 SG Vistra Green 250-320 nd Mid UV 480-620 520 SP 410-540 490 Blue480-620 520 SGOregon Green 488 200-350 nd Mid UV 480-620 524 SP 425-550 496 Blue480-620 524 SG SYBR Gold 250-400 300 Mid UV 500-670 537 SP 400-530 495 Blue500-670 537 SG SYBR Green I 220-540 290 Mid UV 480-630 520 SP 380 Long UV 480-630 520 SP 497 Blue480-630 520 SG SYBR Green II 250-370 254 Short UV 480-640 520SP400-520 497 Blue480-640 520SGSYBR SAFE 250-350 280 Mid UV 500-700 530SPGelStar nd 300 Mid UV nd 527/53 2 rna SP nd 493 Bluend 527/53 2 rna SG SYPRO Orange 250-360 300 Mid UV 500-700 570 SP 380-600 470Blue500-700 570 SG SYPRO Red 230-400 300 Mid UV 550-750 630 LP400-630 550 n/aSYPRO Ruby 250-350 280 Mid UV 535-750 610 UV 350-525 450 Blue 535-750 610 SGRadiant Red 230-330 300 Short or Mid UV 550-700 630 LP 350-600 495 Blue 550-700 630 SGRhodamine 123 425-550 505 Blue 500-650 533 SG Texas Red nd 370 Mid UV 570-750 615 LP 500-630 595 n/aProQ Diamond nd nd Mid UV 550-700 580 UV 475-600 555 n/aProQ Emerald 300 200-400 280 Mid UV 460-640 530 SP Deep Purple300-400ndLong UV520-700600UV460-600530n/aFilters: LP- long pass, SP - short pass, UV mid pass (etbr), SG - needed for some blue light excitation dyes,n/a - not applica