错配修复基因论文：错配修复基因hMLH1和hMSH2在结石至胆管癌变过程中的表达与意义.doc

错配修复基因论文：错配修复基因hMLH1和hMSH2在结石至胆管癌变过程中的表达与意义【中文摘要】通过检测不同病程的肝内结石及伴有肝内胆管结石的胆管癌患者胆管细胞中的hMSH2、hMLH1基因的DNA相对含量值,研究hMSH2、hMLH1基因在肝内胆管细胞癌变过程中的表达与意义。方法：采用荧光定量PCR技术检测正常肝内胆管组织(对照组)、10年以下(结石组1)及10年以上(结石组2)肝内胆管结石病史的胆管组织、伴有胆管结石的胆管癌组织(胆管癌组)中hMLH1、hMSH2基因的DNA相对含量值。结果：(1)hMLH1、hMSH2基因的DNA相对含量值在对照组中最高,结石组高于胆管癌组,且对照组、结石组及胆管癌组三者比较差异具有统计学意义(P0.05)。(2)结石组1的hMSH2、hMLH1基因的DNA相对含量值高于结石组2的hMSH2、hMLH1基因的DNA相对含量值,两者间比较差异具有统计学意义(P0.05)。(3)胆管癌组hMSH2、hMLH1基因的DNA相对含量值比结石组2低(P0.05)。结论：肝内胆管结石长期刺激能导致hMLH1, hMSH2基因表达改变,且随着肝内胆管结石刺激时间的延长而表达下调；胆管癌组hMLH1、hMSH2的表达最低,提示hMLH1、hMSH2是肝内胆管结石长期炎性刺激导致胆管细胞癌变的重要因素之一。【英文摘要】:To detect the relative content of hMSH2 and hMLH1 in different bile duct cells of hepatolithiasis and cholangiocarcinoma patients,research the expression and significance of hMSH2 and hMLH1 in the process of intrahepatic bile duct carcinogenesis.Methods:To detect the relative content of hMSH2 and hMLH1 in normal intrahepatic bile duct tissues (control group)、less than ten years (Calculus group 1) and more than ten years (Calculus group 2) bile duct tissues of hepatolithiasis patients、choledocholithiasis and cholangiocarcinoma tissues(cholangiocarcinoma group) by fluorescence quantitative PCR.Results:The relative content of hMSH2 and hMLH1 is highest in normal group,calculus group is higher than cholangiocarcinoma group,it is significantly different among normal group、calculus group and cholangiocarcinoma group (P0.05).The relative content of hMSH2 and hMLHl of calculus group 1 is higher than calculus group 2,it is significantly different between two groups(P0.05). The relative content of hMSH2 and hMLH1 of cholangiocarcinoma group is lower than calculus group 2.Conclusions:The long-term stimulation of intrahepatic bile duct stones can lead to the different hMSH2 and hMLH1 express, and with the extension of intrahepatic bile duct stimulate, the expression is lower. The expression of hMSH2 and hMLH1 in cholangiocarcinoma group is lowest, prompt that hMLH1, hMSH2 is intrahepatic bile duct with long-term inflammatory stimulation cause bile duct cancer is one of the important factors.【关键词】错配修复基因 肝内 胆管癌 胆管结石【英文关键词】Mismatch Repair Gene Intrahepatic Bile Duct Carcinoma Intrahepatic Calculus【目录】错配修复基因hMLH1和hMSH2在结石至胆管癌变过程中的表达与意义摘要3-4ABSTRACT4第1章 引言6-9第2章 材料和方法9-132.1 实验材料9-112.1.1 实验标本92.1.2 主要试剂92.1.3 主要仪器设备9-102.1.4 主要试剂的配制10-112.2 实验方法11-122.2.1 分组112.2.2 蜡切片中DNA的提取