青枯菌病害的研究进展.doc

, (Ralstonia dolaanacearum)R. dolaanacearum40200, (Agrobacterium tu me faciens),1. Race60412Heliconia344133233333433113421332443551 131322. ;IAACTKEthSequeira1991 eps A-Dops-A-DDennyphc AComplex regulatory networks,CRNSequeiraK 60(1,1)hrphrp343. 31,53.1. 1DNA,6, Danesh67107(AVRDC)WangHawaii7996()6,38,RAPD48YuiHawaii79964RAPD,992324RAPD,2(RA12-12450RA12-291600)9Mangin62103.2. 2000,120,20,20,100,(),113.2.1. DNA3.2.2. ,2,11,122000,AFLPSSR,(RI),3.2.3. ,1997(IBWS),(GBWWG),4. 41Bacillus sp Bacillus spB130B130PH58 PH 6 36 hB1301342ANTI8098AANTI8098A8ANTI8098AANTI8098A5.67585ANTI8098APCRTTC1443 eep( export of extracellular proteins)43 .1 eepTn513Tn5eepPhoA16eep43 .2 eepAWPO41AW PO41 3 108/mlAD4AP7JF1AD4AP7JF120eepAD415% 5. 10 15 20 30AD4 AP7 JF1 CK 0 0 0 30 15 30 20 75 20 65 80 100 20 35 70 80 100 100 100 100AD41AD46. ,DNA,1 ., ,74-86.2 ,. , ,3-12.3 Huang Y,Xu P,Seqyeira L.In: pseudomona solanacearum.Mol Plant-microbe Interact, 1990, 3:48-53.4 Ma Q S,Chang M F,Tang J L etal.Mol Plant-Microbe,1988,4169-1745 ,., ,2001,21(3)66-68.6 Mc Garvey JA,DennyTP,Schell MA,Spatial-temporal and quantitative analysis of growth and EPS I production by Ralstonia solanacearum in resistant and susccptiblc tomato cultivars J, Phytopathology, 1999, 89(12): 1233-1239.7 Danesh D,Young ND,Partial resistance loci for tomato bacterial wilt show differential race specificity J,Tmato Genetics Cooperative Rcport,44:12-13.8 Wang JF,Thoquet J ,Olovier J ,etal,Genetic nanlysis of quantitative resistance loci(QRL)of tomato varicty Hawaii7996 in TaiwanJBacterial wilt disease:Molecular and Ecological Aspects, 1998.246249.9 Yui M,Hirai M ,Nishimura S,etal.Random amplified poly morphic DNA(RAPD) markers forthe selection of tomatoes resistant to bacterial wiltJ.Bulletin the National Research Institute of Vegetables, Ornamental Plants and Tea,1999,14:189198.10 Mangin B,Thoquet P, Oliver J,etal.Temporal and multiple quantitative trait loci analysis ofresistance to bacterial wilt in tomato permit the resolution of linked lociJ. Genetics, 1999, 151(3):11651172.11 Liao Boshou,Host-plant resistance to groundnut bacterial wilt: genetic diversity and enhancement A, ICRISAT,Groundnut Bacterial Wilt in AsialC,India:icrisat,1994,91-96.12 Liao Boshou,Germplasm screening and breeding for resistaqnce to bacterial wilt in Ch