PPD试剂说明书.doc

ppd1 1/30.1ml5ppd6-10mm1ppd20.16-10mm7mm9mm8mm344872+/2 5mm59mm+1019mm+20mm+123428 123451ppd124834hiv5672ppd12345610mm10mm6mm17ppd1234548-72(6) () bcg vaccine for intradermal injection pinei zhusheyong kajiemiao335iu ppdppd48-725mm 5(0.25mg)/1. 1ml1 2.75%1ml(25-26)0.1ml1. 2. 3. 4. 5.2-810/1000/()hbsag 5 g/1631 2 3 410&mug1. >10 miu/ml2. 5 g95%85%3. s19983018686-755-26988688 ():poliomyelitisvaccineindrageecandy(humandiploidcell),live:1g:- 20:2:323464111:1 2 3 :1 237 3:s10870002: 1. 1/42. 31230.5ml46 18240.5ml1. 2. ():188781.5-21295%7-14217-121-22-384 75%0.5ml 5,512(38.3) a61534 a46112233433216433 a2851024126 123 102412715ml253415-308112342193223a314a 5? 1. 2. 13. 80.5ml270.5ml 21. 2. 3. 4. hepatitis a (live) vaccine,freeze-driedppdgf-nepppdgf-nepppdgf-neptaqt4dnadna markertakaradnapsimple-19 ecor/baptakarapegfp-1pcdna3.1(+)pcsc-sppw-net2.1. pdgfdnadnagenbankpdgfgenbankhsn 10c 3,2782bp,ecorf/rbsp106kpndnaf0/r1f1/rpcrpcr1pcr2pcr1pcr2f/rpcr2782bppdgfpcrpsimple-19 ecor/bappsimple-19-pdgf1f05-cttacgggctgtgtgacctt-3f5-atcgatagcatggagaggctacaggtagagt-3f15-tctctgccactgtgccacgct-3r5-ggtaccggcagtcgatggttcgtcttcactc-3r15-agcgtggcacagtggcagaga-31 pdgf promoter2.2. nepncbi genbanknep-actin-actinf 5-acactgtgcccatctacgagggg-3r 5-atgatggagttgaaggtagtttcgtggat-3pcsc-sppw-netapaneppegfp-1apaneppegfp-1t4dnabamhkpnnepkpnbamhnepbamhnotpcdna3.1(+)kpnbamhnept4dnakpnnot2.3. ppdgf-egfpbsp106kpnpsimple-19-pdgfpdgfhindkpnp-egfp -1hindbsp1063ppdgf-egfp f5-agcttcgaattctgcagtcgacggtaccgcgggcccgatr5-cgatcgggcccgcggtaccgtcgactgcagaattcga2.4. ppdgf-nepkpnnotppdgf-egfppdgfpdgfkpnnotnept4dnappdgf-nepppdgf-neprnatrizoldna markerrakaralipofectaminetm2000invitrogennepsanta cruz-actincell signalingnep-actineclsanta cruzsk-n-sh293skov3ecvht-29ppdgf-nepsk-n-sh293skov3ecvht-29sk-n-shsk-n-sh293skov3ecvht-292.5. 1rt-pcrtrizol(invitrogen)rnarevertaid first strand cdna svnthesis kit20lrna 2lpcr50l2ltaq2.5 u10buffer 5l2 mmol/l dntp mixture 2lnep1l-actinll365l943. min9445 s5345 s721 min307210 min9l1.5%2.326neprt-pcr1sk-n-sh2sk-n-sh3293skov3ecvht-293.1. 2western blot8090ripabca12sdspage1 h(1/1000)1h4tbsb310min1 htbsb310min336nepwestern blot1293skov3ecvht-292sk-n-sh3sk-n-shnepamtttakaraelisapromega -a25-35 sigmaa25-35943ul1mm377 a25-354?c20um48h1. mtt4sk-n-shppdgf-nep+appdgf-egfp+aask-n-sh5103/9650%60%ppdgf-nepppdgf-egfpa2535(20mol/l)4810l mtt(5mg/ml)bio-rad570nm634 mtt1sk-n-sh 2ppdgf-nep+a 3ppdgf-egfp+a 4a2. elisaelisask-n-sh bcg vaccine for intradermal injection / 31. 35iu5iu ppdppd48