信息检索(实验报告).doc

综 合 检 索 报 告姓名 _陈双_ 班级 过程自动化_01_ 学号 _0904160102_检索课题（中文） _ 生长素_ （英文）_ IAA 一、检索策略1、检索词：主题词（中文）:生长素（英文）: auxin相关词：（中文）_吲哚乙酸_ （英文）_IAA_副主题词：（中文）_植物 生长_（英文） plant growing_2、构造检索式（布尔逻辑表达式或网络检索式）(生长素 or 吲哚乙酸 or IAA) and 植物 and 生长二、检索来源（本文所查数据库名或其它来源）清华数据库 SOSO网络搜索中国专利数据库 读秀学术搜索万方数据库 欧洲专利网俄亥俄学位论文中心三 、检索结果：分别写出篇名、作者、文献来源（期刊指刊名、 年、卷期；专利指专利号；学位论文指授予单位，标准指标准号等文摘形式）及摘要（有原文请注明），所有数据库均检索近6年的文献。1、中文期刊（找5篇）：（必备）（1）题名：吲哚乙酸对园艺植物生长发育的影响 作者：陈杨利；周玲玲；黄作喜 文献来源：北方园艺，2011年12期 摘要：吲哚乙酸(IAA)是生长素在自然界的主要存在形式。在园艺植物中具有促进种子萌发、扦插生根和诱导花芽分化等重要生理作用。综述了IAA的生理作用及作用机理,阐述了IAA对园艺植物种子、根、茎、株型等生长发育的影响,以期为IAA在园艺植物快速繁殖及优质栽培上得到更合理的应用提供一定的参考。（2）题名：植物生长调节剂IAA、GA和6-BA对芥菜型油菜和甘蓝型油菜富集镉的强化 作者：向言词；官春云；黄璜；严明理；彭秀花 文献来源：农业现代化研究，2010年04期 摘要：重金属植物提取修复效率受到植物体内重金属含量和植物生物量的影响。利用植物生长调节剂可作为强化植物修复效率的一种调控措施。本实验以芥菜型油菜(Brasscia juncea)和甘蓝型油菜(B.napus)为对象,研究了植物生长调节剂IAA、GA和6-BA单独或两两联合作用对这两种油菜富集镉的调控作用。研究结果显示:这3种植物生长调节剂对这两种油菜株高和生物量的影响不同,显著增加芥菜型油菜株高和生物量,却显著降低甘蓝型油菜株高和生物量。同时,这3种植物生长调节剂可显著提高芥菜型油菜和甘蓝型油菜的镉含量和植物积累镉量,这两种类型油菜积累的镉主要集中在茎和叶。植物生长调节剂处理的芥菜型油菜和甘蓝型油菜的镉转运系数和生物富集系数都大于对照,并且可增强油菜吸收镉和促进镉向茎和叶转移。IAA与GA或6-BA联合作用可协同强化油菜提取镉。（3）题名：秋季叶面喷施IAA、6-BA或GA_3对草莓植株的影响 作者：单守明；刘国杰；李绍华；苗鹏飞 文献来源：果树学报，2007年04期 摘要：为了探讨植物生长调节剂对秋季草莓苗植株质量的调控,以法国3号草莓(Fragariaananassa Duch cv.French3)为材料,研究秋季叶面喷施50mg/L IAA、50mg/L6-BA或25mg/L GA3对草莓苗叶片光合作用、活性氧代谢和植株质量的影响。结果表明,晚秋叶面喷施这3种植物生长调节剂显著提高了叶片的净光合速率和叶绿素a、b含量,提高了SOD和CAT酶活性,同时降低了MDA和活性氧含量。另外,这3种植物生长调节剂处理显著增加草莓茎和根系的干重,使根冠比增加,IAA和6-BA处理还显著提高平均单株花量。因此,秋季叶面喷施50mg/LIAA和50mg/L6-BA可提高草莓苗植株质量。（4）题名：生长素对植物生长发育调节作用的模拟 作者：朱庆生；侯凌锋；吕中华 文献来源：中国农业科技导报，2008年04期 摘要：为有效地模拟真实植物的生长过程,综合考虑植物外部形态、生理机制和环境因素的相互影响,基于开放L系统,对植物激素生长素对植物生长调节作用进行建模,真实再现植物在内外因素影响下的生长发育形态,并实现植物生长的动态可控性。（5）题名：海带中3-吲哚乙酸对海洋微藻生长代谢的影响 作者：李铁松；王长海 文献来源：辽宁师范大学学报(自然科学版)，2010年01期 摘要：利用反向高效液相色谱、UV光谱扫描和质谱方法对海带(Laminaria japonicaAresch.)提取物中的3-吲哚乙酸进行定性和定量分析,结果表明海带提取物中含有3-吲哚乙酸.在此基础上,利用反向高效液相色谱方法对3-吲哚乙酸进行制备,并研究其对小球藻(Chlorellasp.)、盐藻(Dunaliellasalina)、角毛藻(Chaetoceros muelleri)、紫球藻(Porphyridiumcruentum)和球等边金藻(Isocrydid galbana)5种海洋微藻生长代谢的影响.实验表明海带3-吲哚乙酸浓度为0100mol/L时,小球藻、盐藻和紫球藻细胞生长均被促进;海带3-吲哚乙酸浓度对小球藻和紫球藻胞内蛋白质含量影响显著,对小球藻叶绿素含量影响极显著.研究为海带生长素类物质活性及海洋中大型藻与微藻生长关系的研究奠定了基础.更多还原2、外文期刊（找5篇）：（必备）（1）题名：Rhizobacterial potential to alter auxin content and growth of Vigna radiata (L.) 作者：Basharat Ali; Anjum Nasim Sabri; Shahida Hasnain 文献来源：World Journal of Microbiology and Biotechnology，2010年08期 摘要：Abstract: Potential of non-symbiotic plant growth promoting rhizobacteria (PGPR) to influence the endogenous indole-3-acetic acid (IAA) content and growth of Vigna radiata (L.) was evaluated. The bacterial strains used belonged to Pseudomonas, Escherichia, Micrococcus and Staphylococcus genera. All strains were able to produce IAA (1.168.22gml1) in the presence of 1,000gml1 of l-tryptophan as revealed by gas chromatography and mass spectrometric (GCMS) analysis. However, strains exhibited variable results for other growth promoting traits such as phosphate solubilization and siderophore or hydrogen cyanide production. Bacterial IAA production showed significant positive correlation with endogenous IAA content of roots (r=0.969; P=0.01) and leaves (r=0.905; P=0.01) under axenic conditions. Bacterization of V. radiata seeds significantly enhanced shoot length (up to 48.10%) and shoot fresh biomass (up to 43.80%) under fully axenic conditions. Bacterial strains applied under wire-house conditions also improved shoot length, number of pods, and grain weight up to 58, 65, and 17.15% respectively, over control. Hence, free living (non-symbiotic) PGPR have the ability to influence endogenous IAA content and growth of leguminous plants.（2）题名：Changes in expansin gene expression, IAA content, and extension growth of leaf cells in maize plants subjected to salinity 作者：D. S. Veselov; I. B. Sabirzhanova; B. E. Sabirzhanov; A. V. Chemeris 文献来源：Russian Journal of Plant Physiology，2008年01期 摘要：Abstract:Effects of salinity of nutrient solution on expression of ZmEXPA1 expansin gene and leaf growth were studied on maize plants (Zea mays L.). Rapid activation of the gene transcription was shown to precede the resumption of extension growth in leaf cells under water deficit induced by NaCl salinity. Auxins were found to accumulate in leaves during salinity treatment, the accumulation being faster than activation of ZmEXPA1 transcription. In addition, exogenous IAA was shown to enhance the gene expression. Our results indicate that the hormone is involved in regulation of cell extension growth at high salinity through the expression of expansin gene.（3）题名：Auxin Biosynthesis and Its Role in Plant Development 作者：Yunde Zhao 文献来源：Annual Review of Plant Biology，2010年06期 摘要：Indole-3-acetic acid (IAA), the main auxin in higher plants, has profound effects on plant growth and development. Both plants and some plant pathogens can produce IAA to modulate plant growth. Although the genes and biochemical reactions for auxin biosynthesis in some plant pathogens are well understood, elucidation of the mechanisms by which plants produce auxin has proven to be difficult. So far, no single complete pathway of de novo auxin biosynthesis in plants has been firmly established. However, recent studies have led to the discoveries of several genes in tryptophan-dependent auxin biosynthesis pathways. Recent findings have also determined that local auxin biosynthesis plays essential roles in many developmental processes including gametogenesis, embryogenesis, seedling growth, vascular patterning, and flower development. In this review, I summarize the recent advances in dissecting auxin biosynthetic pathways and how the understanding of auxin biosynthesis provides a crucial angle for analyzing the mechanisms of plant development.（4）题名：Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development 作者: H. Tanaka; P. Dhonukshe; P. B. Brewer; J. Friml 文献来源：Cellular and Molecular Life Sciences，2006年12期 摘要：Abstract.:The plant hormone auxin plays crucial roles in regulating plant growth development, including embryo and root patterning, organ formation, vascular tissue differentiation and growth responses to environmental stimuli. Asymmetric auxin distribution patterns have been observed within tissues, and these so-called auxin gradients change dynamically during different developmental processes. Most auxin is synthesized in the shoot and distributed directionally throughout the plant. This polar auxin transport is mediated by auxin influx and efflux facilitators, whose subcellular polar localizations guide the direction of auxin flow. The polar localization of PIN auxin efflux carriers changes in response to developmental and external cues in order to channel auxin flow in a regulated manner for organized growth. Auxin itself modulates the expression and subcellular localization of PIN proteins, contributing to a complex pattern of feedback regulation. Here we review the available information mainly from studies of a model plant, Arabidopsis thaliana, on the generation of auxin gradients, the regulation of polar auxin transport and further downstream cellular events.（5）题名:The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses 作者：Akihiro Ueda; Pinghua Li; Yue Feng; Meenu Vikram; Sewon Kim; Chang Ho Kang; Jae Sook Kang; Jeong Dong Bahk; Sang Yeol Lee; Toshiyuki Fukuhara; Paul E. Staswick; Alan E. Pepper; Hisashi Koiwa 文献来源：Plant Molecular Biology，2008年08期 摘要：Abstract:More than 20 genes in the Arabidopsis genome encode proteins similar to phosphatases that act on the carboxyl-terminal domain (CTD) of RNA polymerase II. One of these CTD-phosphatase-like (CPL) proteins, CPL2, dephosphorylates CTD-Ser5-PO4 in an intact RNA polymerase II complex and contains a double-stranded (ds)-RNA-binding motif (DRM). Although the dsRNA-binding activity of CPL2 DRM has not been shown to date, T-DNA insertion mutants that express CPL2 variants lacking either a part of DRM (cpl2-1) or the entire DRM (cpl2-2) exhibited leaf expansion defects, early flowering, low fertility, and increased salt sensitivity. cpl2 mutant plants produced shorter hypocotyls than wild-type plants in the light, but were indistinguishable from wild type in the dark. CPL2 was expressed in shoot and root meristems and vasculatures, expanding rosette leaves, and floral organs suggesting a focal role for growth. Microarray and RT-PCR analyses revealed that basal levels of several auxin-responsive transcripts were reduced in cpl2. On the other hand, the levels of endogenous auxin and its conjugates were similar in wild type and cpl2. Overexpression of ARF5 but not all activator ARF transcription factors restored the auxin-responsive DR5-GUS reporter gene expression and the leaf expansion of cpl2 mutant plants but not early flowering phenotype. These results establish CPL2 as a multifunctional regulator that modulates plant growth, stress, and auxin responses.3、专利文献（中外文各找2篇）：（工科必备）（1）题名：生长素释放肽类似物 申请(专利)号: CN200680035324.2 公开(公告)号: CN101282738 申请专利权人:科学研究和应用咨询股份公司 发明(设计)人: 董正欣;MD卡勒;Y舍恩;JM科姆斯托克 主分类号：A61K38/00(2006.01) 分类号: A61K38/00(2006.01);C07K1/00(2006.01) 优先权:2005.09.28 US 60/721,557; 2005.12.09 US 60/748,904; 2005.12.15 US 60/750,771 摘要：本发明包含如下所述的根据式(I)或(II)的肽基类似物和其可药用盐：(R2R3)A1A2A3A4A5A6A7A8A9A10A11A12A13A14A15A16A17A18A19A20A21A22A23A24A25A26A27A28R1，其中对于式(I)和(II)中每一个的A1至A28和R1至R3的定义在说明书中提供，和包含有效量的具有激动剂或拮抗剂生长素释放肽活性的式(I)化合物的药物组合物及其治疗和非治疗用途。（2）（中国专利数据库）题名：用于在植物中诱导遗传单性结实的方法申请(专利)号: CN97193072.4 公开(公告)号: CN1213405 申请专利权人: 以色列国农业部 发明(设计)人: R巴格;Y萨勒特 主分类号：C12N15/82 分类号: C12N15/82;A01H5/00 优先权: 1996.02.14 IL 117139 摘要：本发明提供一种用于在植物中产生遗传单性结实的改进方法,该方法包括以下步骤:提供一个盒,该盒包括在植物中编码调节植物生长素作用的DNA序列(如rolB基因的序列) 和在开花和果实早期发育之间对子房具有特异性的启动子,该启动子控制DNA序列;将该盒引入植物中。优选地,通过植物材料(包括种子产生的子叶)的转化引入所说的DNA,以及再生所转化的植物。优选方法的步骤也包括筛选兼性或者专性单性结实特征的植物。（3）（万方数据库） 题名：PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME申请(专利)号: EP20090783222 公开(公告)号: EP2334797(A1) 申请专利权人: BASF PLANT SCIENCE GMB 发明(设计)人: HATZFELD YVESFRANKARD VALERIEREUZEAU CHRISTOPH 主分类号：C12N15/82,C,C12,C12N,C12N15 分类号: C12N15/82,A01H5/00,C,A,C12,A01,C12N,A01H,C12N15,A01H5 摘要：The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an ASPAT (Asparatate AminoTransferase) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an ASPAT polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides hitherto unknown ASPAT-encoding nucleic acids and constructs comprising the same, useful in performing the methods of the invention. Furthermore, the present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by increasing expression in a plant of a nucleic acid sequence encoding a MYB91 like transcription factor (MYB91 ) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding an MYB91 polypeptide, which plants have increased yield- related traits relative to control plants. The invention additionally relates to nucleic acid sequences, nucleic acid constructs, vectors and plants containing said nucleic acid sequences. Even furthermore, the present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GASA (Gibberellic Acid-Stimulated Arabidopsis). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GASA, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. Yet furthermore, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an AUX/IAA (auxin/indoleacetic acid) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding IAA polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides constructs comprising AUX/IAA-encoding nucleic acids, useful in performing the methods of the invention.（4）（万方数据库） 题名：SUPPRESSING PLANT PATHOGENS AND PESTS WITH APPLIED OR INDUCED AUXINS申请(专利)号: EP20040781404 公开(公告)号: 申请专利权人: STOLLER ENTPR INC (US) 发明(设计)人: STOLLER JERRY H (US) 主分类号：A01N25/26,A,A01,A01N,A01N25 分类号: A01N25/26,A01N37/10,A01N43/36,A01N43/34,A01N31/08,A01N43/38,A01N57/14,A,A01,A01N,A01N25,A01N37,A01N43,A01N31,A01N57 摘要：The present invention is directed to methods for inhibiting the growth of disease organisms, particularly fungi and bacteria, on plant tissues. The present invention is also directed to methods for inhibiting the infestation of plants by insects and larva, particularly sucking and chewing insects. These methods are achieved by applying an auxin or a plant growth regulator (PGR) which will effect the level of auxin in the plant tissue to the seeds or tubers of the plant prior to planting or to the roots, foliage, flowers or fruit of the plant after planting. The auxin or PGR is applied in an amount effective to inhibit growth of the disease organisms or insects, but in an amount insufficient to negatively effect growth of the plant tissues. The auxin may be applied as a natural auxin, synthetic auxin, auxin metabolite, auxin precursor, auxin derivative or a mixture thereof. The presently preferred auxin is indole-3-acetic acid (IAA). The auxin or PGR may be applied to the seeds, tubers or plant tissues. Seeds or tubers may be sprayed with or immersed in an aqueous solution containing the auxin or PGR. Conventional spraying and drip irrigation systems may be used to apply an aqueous solution containing an auxin or PGR to plant tissues. The auxin or PGR may also be applied to the plant tissues as a powder or may be encapsulated within a biologically compatible material to provide slow release to the roots of the plant. The plant tissues may be dusted with a powder, including the auxin or PGR. The encapsulated auxin may be placed in the root zone for uptake of the auxin or PGR by the roots.4、学位论文（中外文各找2篇）：（必备）（1）题名：生长素信号对植物生长与系统性获得抗性的调控作用 作者：王晓莉（硕士） 授予单位：南京农业大学 摘要：生长素是一类参与植物发育过程的典型植物激素,这种非蛋白的简单分子参与调控:胚的形态建成、细胞分裂与伸长、维管组织的分化、侧根的起始生成、向地性和向光性等。同时,在分子水平上,生长素还调控着无数基因的表达。近年来的研究,尤其是生长素受体转运抑制响应蛋白(transport inhibitor response protein 1,TIR1)和疑似受体生长素结合蛋白(auxin binding protein 1,ABP1)的发现,使得生长素信号通路得到简洁清晰的解析。最近的研究发现,水杨酸全面抑制生长素相关基因的表达,且水杨酸对生长素信号通路的抑制是水杨酸介导的抗病防卫反应机制的一部分,说明生长素在植物防卫过程中也起着重要作用。然而,这样一个看似简单的信号通路是如何参与调控植物中无数的发育过程的,生长素又是如何共同调控植物生长与防卫的,这些问题还有待进一步解析。另外,植物非激素信号对生长与防卫具有重要调控作用,其中,核黄素不仅可以诱导植物抗病性,还能促进植物生长。在表达软体海龟核黄素受体基因的转基因植物中,核黄素含量下降,植物生长加快、抗病性提高,但其调节机制还不了解。本文着重研究生长素信号对植物生长和抗病性的调控作用;为了进一步探讨激素信号与核黄素对植物生长发育和抗病性的不同调控作用,本文还构建了核黄素受体与荧光蛋白的融合蛋白,产生了转基因拟南芥,用于进一步研究。 1.一种生长素抑制蛋白调控烟草矮化突变体的组成性系统获得抗性 植物中的系统获得抗性(systematic acquired resistance,SAR)是由多种激发子诱导产生的。在对激发子的反应过程中,锚蛋白NPR1(non-expressor of PR)的主要功能是调控植物防卫基因的表达,这些基因包括SAR相关基因以及像hsr203和hinl这样的过敏反应相关基因。这些基因编码防卫产物包括病程相关(pathogenesis-related,PR)蛋白、几丁质酶或者其它可以抵抗病原物的酶。这些产物共同作用来防止病原物的侵染和致病,从而使植物产生抗病表型。因而,防卫反应基因的表达和系统抗性的产生被认为是SAR的主要特征。在特殊环境之下,一旦植物的遗传特性被修饰,SAR的特征就会通过繁殖变成组成性的和可遗传的。在这种情况下,组成性表达SAR(cSAR)可以遗传到植物的有性后代中,抗性反应基因的表达可以不依赖激发子的存在。 人们已经应用了许多方法来修饰植物的遗传特性和改变SAR的特性。一种植物工程方法是将防卫调控基因融合到一个组成性表达的启动子上,诸如花椰菜花叶病毒来源的35S启动子。遗传突变广泛的应用于标记包括正向及负向调控抗病反应的植物基因。一种常规的诱变方法是在植物的胚体的无性繁殖过程中,如细胞或组织培养和植物再生过程,对其进行无性系变异。这个过程能够使由SAR激发子引发的植物遗传变异稳定下来,从而改变SAR特性的遗传方式。 为了研究植物生长和系统获得抗性的共同调控作用,我们对野生型烟草(Nicotiana tabacum)进行体细胞无性系变异处理,得到组成性表达SAR的突变体cesl-l。与野生型相比,cesl-l表型矮化但却对真菌的侵染表现抗性。这种突变体能组成性的表达植物抗病反应基因,但是却不能表达植物生长途径中的伸展素expansin基因。回交的遗传分析表明,cesl-l是在野生型位点上的单基因显性突变。差异显示表明,在cesl-l中存在一个与编码生长素抑制蛋白的基因NtARPl一致的转录本,而野生型烟草中缺少此转录本。我们通过cDNA末端快速扩增(rapid amplification ofcDNA ends,RACE)的方法克隆到NtARPl基因,并检测了生长素对基因表达的影响及植物中NtARPl的蛋白产物。将NtARPl基因进行瞬时表达,发现NtARPl定位在cesl-l的细胞核上。经过吲哚乙酸IAA处理的cesl-l,其NtARPl的表达和NtARPl蛋白产物的产生均受到抑制。我们进而对NtARPl在调控烟草的生长防卫功能上进行了研究。当突变体cesl-l中的NtARPl被沉默时,其生长恢复了野生型的表型,但是丧失了组成性表达SAR的特性。cesl-l中的IAA含量低于野生型,然而,外部施用IAA可以使cesl-l中IAA含量恢复到野生型水平。实验结果表明,存在一条生长素信号通路负向调控植物的生长而正向调控防卫。 2.核黄素受体基因转基因拟南芥的产生和鉴定 核黄素在生物体内形成黄素单核苷酸(flavin mononucleotide,FMN)及黄素腺嘌呤二核苷酸(flavin adenjne dinucleotide,FAD),它们是黄素酶的辅酶。核黄素所形成的辅酶参与机体组织维生素C抗氧化作用的呼吸电子传递及氧化还原过程,参与植物抗氧化及过氧化过程,从而影响氧化性损伤及后续过敏反应过程中活性氧中间体(reactive oxygen species,ROS)的产生,而ROS是过敏细胞坏死的重要信号,能调节生长、抗病、抗虫、抗逆,所以核黄素与植物免疫反应有关。外施核黄素可以诱导植物抗病性。植物中的核黄素水平可能对抗病防卫和生长发育过程有影响。因此,如果能够控制核黄素水平就能相应控制这些重要过程,提高植物抗病防卫的能力并促进植物生长发育。以此为切入点,可以研究核黄素参与的植物防卫和生长调控的交叉点。 本实验室已经克隆到了中华鳖的核黄素受体(riboflavin binding protein,RfBP)基因。本研究将绿色荧光蛋白(green fluorescent protein,GFP)基因融合构建的核黄素结合蛋白(riboflavin binding protein,RfBP)基因转入拟南芥中,来研究RfBP蛋白在植物细胞内的定位,进而研究RfBP蛋白在植物发育和防卫信号传导中的作用。将来自软体海龟的RfBP基因连入植物表达载体pBI121中,用水母绿色荧光蛋白(greenfluorescent protein,GFP)标记,构建了植物重组表达质粒pBI121-RfBP-GFP,由农杆菌Agrobacterium tumef