科技查新范例(报告).doc

附件目录中文文献1从微生物中提取超氧化物歧化酶的研究/胡佩/ 四川师范大学学报(自然科学版) 1995年05【摘要】 超氧化物歧化酶，简称SOD（Superoxide dismutase），是一种应用价值大、广泛存在于生物体细胞内的金属酶本实验以酿酒酵母和嗜热脂肪芽孢杆菌为出发菌株，进行抗H_2O_2性能的筛选，选出耐受性好的菌株经2L发酵罐发酵后，提取到了热稳定性好的Cu, Zn-SOD和Fe-SOD,酶活力为3292和3547u/mL,其比活力为3457和3084u/mg2 从微生物中提取超氧化物歧化酶的研究 / 张远琼 / 宜宾学院学报 1996年02期【摘要】 超氧化物歧化酶,简称SOD(Superoxide dismutase),是一种应用价值大、广泛存在于生物体细胞内的金属酶,本实验以酿酒酵母和嗜热脂肪芽孢杆菌为出发菌株,进行抗H_2O_2性能的筛选,选出耐受性好的菌株经2L发酵罐发酵后,提取到了热稳定性好的(?)Zn-SOD和Fe-SOD,酶活力为3292和3547u/ml,其比活力为3457和3084u/mg.4 高产超氧化物歧化酶芽孢杆菌C328菌株产酶培养基的研究 / 丁学知 高必达 / 湖南师范大学自然科学学报.2003.026(004).-65-69 【文摘】 研究了芽孢杆菌(Bacillus cereus)C328 SOD(Superoxide dismutase)生产菌株在发酵培养过程中培养基种类、碳氮比以及补充碳氮源对产酶的影响结果表明，C328菌株发酵产酶率与培养基种类、复配碳氮比有密切关系,培养基浓度为10时，对菌株生长及产酶有明显的促进作用，超过13则抑制SOD酶产生筛选出最佳产酶发酵农副产品培养基和优化配比组合产酶培养基。5 重组人锰超氧化物歧化酶工程菌高效表达培养工艺的研究 / 张艳红 廖晓全 等 / 中国药学杂志.2002.037(001).-59-62 【文摘】 目的 确定重组人锰超氧化物歧化酶（rhMn-SOD）工程菌高效表达的最适培养工艺条件。方法 通过摇瓶培养研究了包括接种量、诱导时机、Mn2+浓度、诱导后培养时间、发酵过程中pH变化以及初始pH值对发酵的影响。结果 该工程菌的最佳接种量为3%，最佳诱导时机为接种后3h，确定Mn2+浓度为6000molL-1，诱导后培养5h，在培养过程中当pH为6.83时外源蛋白可获得高效表达，初始pH确定为8.0。结论：诱导和培养基的pH是影响工程菌发酵的主要因素，尤其是Mn2+的加入表达有活性的rhMn-SOD是必需。6嗜热脂肪芽孢杆菌抗氧化酶相关的研究 / 丁学知 夏立秋 / 湖南师范大学自然科学学报.1999.022(004).-73-77 【文摘】 从一些地区的温泉和近热源的土壤以及污水中分离到株嗜热脂肪芽孢杆菌，并对其生长量，蛋白含量和抗氧化化酶活力及其电泳图谱特征进行了研究，结果表明，不同菌株的生长量与蛋白一之间呈负相关趋势；POD、CAT和SOD3种抗氧化酶之间具有密切的相关性，在细胞内组成了一个连锁互补的重要的抗氧化酶体系抗氧化酶活性高的T926菌株表现出具有很高的耐高温的能力。 7嗜热脂肪芽孢杆菌抗氧化酶相关性研究 / 丁学知 陈德 / 常德高等专科学校学报.1996.008(001).-4-7 8芽孢杆菌超氧化物歧化酶的热和pH稳定性研究 / 周国庆 夏立秋 / 常德师范学院学报：自然科学版.1999.011(003).-62-63,67 【文摘】 研究了从质芽孢杆菌中提取的Fe-SOD纯酶的热和PH稳定性。结果表明，在25、35下保温20-95min酶活略出现增高，45保温50min和80min其酶活分别为原酶的81.0%和70.0%；为6-10时酶活稳定。9蜡质芽孢杆菌超氧化物歧化酶稳定性研究 / 夏立秋 王文龙 / 常德高等专科学校学报.1996.008(001).-8-10 【文摘】 采用化学动力学方法首次测定了蜡质芽孢杆菌SOD稳定性，结果表明，蜡质芽孢杆菌SOD的t0.9为55.97/min, t1/2为369.40min，该酶显示出具有良好的稳定性，提示可作为一种SOD药用酶的重要资源，具有潜在的重要药用价值。10一株耐高温SOD产生菌的筛选及酶学特性 / 王忠彦. 黄英. 胡永松. 胡佩/ 微生物学报 1997，37(4)：P307-311 【摘要】 超氧化物歧化酶(Superoxide dismutase简称SOD)是生物体防御氧化损伤的金属酶。具有治疗炎症、抗辐射损伤、防癌、抗衰老等广泛药用前景。另外在植物抗逆和生物固氮等方面也有重要作用。自1969年McCord和Fridovich发现该酶以来,人们已从多种动植物和微生物中分离了此酶。SOD的基因工程也取得了令人瞩目的成就。相对而言,动植物Cu、Zn-SOD的研究较活跃,而微生物SOD,特别是Fe-SOD的研究较薄弱。本文报道耐高温SOD产生菌的筛选、鉴定结果,并对酶的稳定性和酶学特性作了研究。 11球形芽孢杆菌C3-41超氧化物岐化酶的特性 / 郑滔,刘娥英,蔡全信,袁志明,张用梅 / 微生物学通报 1998年06期 【摘要】 本文研究了球形芽孢杆菌（Bacillus sphaericus）C3-41超氧化物歧化酶（SOD）的产生条件和部分特性。当C3-41菌株处于孢子囊中期时为产SOD酶高峰期，在30下的平板培养物及培养基起始pH为中性（pH7.0）时产生的SOD酶比活最高，经硫酸铵分级沉淀，DEAE-32离子交换层析和SephadexG-100凝胶过滤提纯了SOD酶。此酶属Mn-SOD，在2535和pH59范围内较稳定，但在55下10min完全失活。 12蜡样芽孢杆菌分泌超氧化物歧化酶特性的研究 / 刘勇,张恩平,龚月生 /饲料工业 2004年04期 【摘要】 试验利用氮兰四唑(NBT)比色法测超氧化物歧化酶(SOD)活性,对一株用于生产的蜡样芽孢杆菌(Bacillus cereus,简称B.C菌)的SOD分泌特性做了初步研究。结果表明:培养温度、时间及培养基pH值、盐浓度对B.C菌分泌SOD都有不同程度的影响。菌株的繁殖速度随时间推移而变化,呈现先升后降的趋势,24h达到繁殖旺盛期;不同培养时间,菌体分泌SOD的活性不同。随培养时间延长,胞内酶活性表现出先升后降的变化趋势,20h时活性最高;随培养温度升高,菌株分泌SOD活性表现出先升后降的趋势;30条件下培养时,胞外胞内及总酶活性均为最高;培养基pH值在5.07.0范围内时,菌株胞内133株蜡样芽孢杆菌生理特性及SOD发酵条件的研究 / 杨保伟,来航线,盛敏 / 西北农林科技大学学报(自然科学版) 2004年03期 【摘要】 对3株蜡样芽孢杆菌的培养特征、生理生化实验、喂养实验、动物毒性实验、生长特性及其性质进行了研究,并对其SOD液体发酵条件进行了分析。结果表明,3株蜡样芽孢杆菌均为有益菌;pH为7.5时,3株菌在2530发酵60h后酶活均达到最高;在3545发酵2436h后酶活最高。通过比较发现,02号菌株的SOD活性在整个培养过程中均比较高,且酶活值比较稳定,是生产微生态制剂的理想菌株。英文文献 1Superoxide dismutase biosynthesis by two thermophilic bacteria / Gligic, L; Radulovic, Z; Zavisic, G / Enzyme and Microbial Technology Enzyme Microb. Technol. Vol. 27, no. 10, pp. 789-792. Dec 2000. 【Abstract 】Some high-molecular weight antioxidant defense system components of two thermophilic bacteria isolated from spa waters of Serbia (Yugoslavia) and identified as Bacillus stearothermophilus and Thermothrix sp. were studied. In addition to superoxide dismutase (SOD; EC 1.15.1.1), qualitative analyses demonstrated the presence of catalase (EC 1.11.1.6), peroxidases and oxidases in both bacterial strains. Cell-free extracts were subjected to nondenaturing polyacrylamide gel electrophoresis (PAGE) and SOD activity in the eluates of the corresponding bands was examined in the presence of several specific inhibitors. A slight decrease of SOD activity observed in the presence of 0.3 M potassium cyanide and its complete insensitivity to hydrogen peroxide (5 mM) and sodium azide (20 mM) action suggest that the enzyme occurring in the two thermophiles represents Mn SOD. A high SOD activity recorded in cell-free extracts strongly recommends these two bacterial strains as potential producers of this important antioxidant defense system component at industrial scale.2Molecular cloning and nucleotide sequence of the superoxide dismutase gene and characterization of its product from Bacillus subtilis / Inaoka, T; Matsumura, Y*; Tsuchido, T / Journal of Bacteriology J. Bacteriol. Vol. 180, no. 14, pp. 3697-3703. Jul 1998.【Abstract 】 Bacillus subtilis was found to possess one detectable super-oxide dismutase (Sod) in both vegetative cells and spores. The Sod activity in vegetative cells was maximal at stationary phase. Manganese was necessary to sustain Sod activity at stationary phase, but paraquat, a superoxide generator, did not induce the expression of Sod. The specific activity of purified Sod was approximately 2,600 U/mg of protein, and the enzyme was a homodimer protein with a molecular mass of approximately 25,000 per monomer. The gene encoding Sod, designated sodA, was cloned by the combination of several PCR methods and the Southern hybridization method. DNA sequence analysis revealed the presence of one open reading frame consisting of 606 bp. Several putative promoter sites were located in the upstream region of sodA. The deduced amino acid sequence showed high homology with other bacterial manganese Sods. Conserved regions in bacterial manganese Sod could also be seen. The phenotype of double mutant Escherichia coli sodA sodB, which could not grow in minimal medium without supplemental amino acids, was complemented by the expression of B. subtilis sodA.3 Purification and properties of superoxide dismutase from Bacillus circulans . / Lee, Tae Ho; Lee, Sang Ok / Agricultural and Biological Chemistry AGRIC. BIOL. CHEM., vol. 52, no. 6, pp. 1361-1367, 1988 【Abstract 】Superoxide dismutase (SOD) was purified from Bacillus circulans IFO 3329 to an electrophoretically homogeneous state and partially characterized. Metal ions had little effect on the SOD activity. The absorption maximum in the visible range was found at 475 nm, and the enzyme was red-purple in color and cyanide-insensitive. These results suggest that the enzyme is a Mn-containing SOD.4 Isolation of manganese-containing superoxide dismutase from Bacillus subtilis . / Tsukuda, K; Kido, T; Shimasue, Y; Soda, K / Agricultural and Biological Chemistry AGRIC. BIOL. CHEM., vol. 47, no. 12, pp. 2865-2870, 1983 【Abstract 】 Bacillus subtilis is regarded as one of the most important bacteria in the food industry. Next to Mycobacterium lepraemurium, B. subtilis (IFO 3022) is the best bacterial producer of superoxide dismutase. The B. subtilis superoxide dismutase was purified 40-fold to homogeneity from a cell-free extract using ammonium sulfate fractionation and DEAE-Toyopearl and Sephadex G-150 chromatography. The enzyme has a molecular weight of 45,000 and consists of two subunits identical in molecular weight. The enzyme, which exhibits absorption maxima at 280 and 470 nm, contains 1.13 g atoms of Mn per mol of enzyme as the catalytically active metal.5 Purification and properties of mangano-superoxide dismutase from a strain of alkaliphilic Bacillus. / Hakamada, Y; Koike, K; Kobayashi, T; Ito, S / Extremophiles : life under extreme conditions, 1997 May, 1(2):74-8 【Abstract】 A mangano-superoxide dismutase (EC 1.15.1.1) was purified to homogeneity from a strain of alkaliphilic Bacillus for the first time. The purified protein, with an isoelectric point of pH 4.5, had a molecular mass of approximately 50 kDa and consisted of two identical subunits (25 kDa). The N-terminal amino acid sequence was Ala-Tyr-Lys-Leu-Pro-Glu-Leu-Pro-Tyr-Ala-Ala- Asn-Ala-Leu-Glu-Pro-Hi s-Ile- Asp-Glu-Ala. The optimum pH and temperature for the reaction were 7.5 and 35 degrees C, respectively. The properties of the superoxide dismutase were compared with those of the enzyme from thermophilic Bacillus stearothermophilus.6 Pharmaceutical compositions containing superoxide dismutase from Bacillus stearothermophilus and Bacillus caldotenax. / Atkinson-A; Bown-K-J; Brehm-J-K; Chambers-S-P; Minton-N-P / Official-Gazette-of-the-United-States-Patent-and-Trademark- Office-Patents. June 30, 1998; 1211 (5): 4927.7 Study on the intracellular superoxide dismutase produced by Bacillus circulans. / Lee,-S.O.; Lee,-T.H. / Korean-Journal-of-Applied-Microbiology-and-Bioengineering. (Dec 1987). v. 15(6) p. 381-387.8 Process for preparing a fermentation product having SOD activity using a microorganism and a beverage containing the same / Koh, KJ; Jang, BY; Lee, JH; Lee, KP; Kong, UY / Cheil Jedang Corporation 【Abstract 】 A process for preparing a fermentation product having superoxide dismutase (SOD) activity, which can reduce blood alcohol level and eliminate foul alcohol breath using a novel microorganism of the Bacillus species, and a beverage containing the fermentation product.9 Proteomic analysis of a thermostable superoxide dismutase from Bacillus stearothermophilus TLS33. / Sookkheo, B; Sinchaikul, S; Thannan, H; Thongprasong, O; Phutrakul, S; Chen, ST / Proteomics, 2002 Sep, 2(9):1311-5【Abstract 】 Thermophilic bacterium Bacillus stearothermophilus TLS33 isolated from a hot spring in Chiang Mai, Thailand produces an extracellular superoxide dismutase (SOD). SOD is a free radical metabolizing enzyme that protects the cell membrane from damage by the highly reactive superoxide free radicals. To identify the secreted SOD, we used the systematically proteomic approaches of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis and database searching. The bacterium was grown in a medium containing 0.1% w/v yeast extract and 0.1% w/v tryptone in100% v/v base mixture at 65 degrees C for 72 h, by assessing their growth by protein and SOD activity. The bacterium produced the highest SOD activity at 65 degrees C for 48 h and the extracellular SOD was run on 2-D PAGE using broad range pH 3-10 immobilized pH gradients (IPGs) and narrow range pH 4-7 IPGs. The isoelectric point and molecular mass of the extracellular SOD were approximately 5.8 and 28 kDa, respectively. In addition, the NH(2)-terminal amino acid sequence was found to be P-F-E-L-P-A-L-P-Y-P-Y-D-A-L-E-P-P-I-I-D, which had a homology of approximately 85% to the Mn-SOD family and 65% to the Fe-SOD family.10 Three-dimensional structure of manganese superoxide dismutase from Bacillus halodenitrificans, a component of the so-called green protein. / Liao, J; Liu, MY; Chang, T; Li, M; Le Gall, J; Gui, LL; Zhang, JP; Jiang, T; Liang, DC; Chang, WR / Journal of structural biology, 2002 Sep, 139(3):171-80 【Abstract】 A so-called green protein has been purified from a moderate halophilic eubacterium, Bacillus halodenitrificans (ATCC 49067), under anaerobic conditions. The protein, which might play an important role in denitrification, dissociates mainly into two components after exposure to air: a manganese superoxide dismutase (GP-MnSOD) and a nucleoside diphosphate kinase. As a first step in elucidating the overall structure of the green protein and the role of each component, the 2.8-A resolution crystal structure of GP-MnSOD was determined. Compared with other manganese dismutases, GP-MnSOD shows two significant characteristics. The first is that the entrance to its substrate channel has an additional basic residue-Lys38. The second is that its surface is decorated with an excess of acidic over basic residues. All these structural features may be related to GP-MnSODs high catalytic activity and its endurance against the special cytoplasm of B. halodenitrificans. The structure of GP-MnSOD provides the basis for recognizing its possible role and assembly state in the green protein.11 Crystallization and preliminary crystallographic analysis of manganese superoxide dismutase from Bacillus halodenitrificans. / Liao, J; Li, M; Liu, MY; Chang, T; Le Gall, J; Gui, LL; Zhang, JP; Liang, DC; Chang, WR / Biochemical and biophysical research communications, 2002 May 31, 294(1):60-2 【Abstract】 Manganese superoxide dismutase (GP-MnSOD), a component of the so-called green protein (green protein complex) from the facultative anaerobic halodenitrifier Bacillus halodenitrificans, has been crystallized using the hanging-drop vapor diffusion method. Crystals have unit-cell parameters a=b=93.4 A, c=65.0 A, and belong to the space group P4(3)2(1)2. Preliminary analysis indicates there is one monomer in each asymmetric unit. The structural information from this enzyme will enrich our knowledge on its high catalytic activity and its possible role in green protein complex.12Crystal structure of manganese superoxide dismutase from Bacillus stearothermophilus at 2 multiplied by 4 Angstrom resolution. / Parker, MW; Blake, CCF / Journal of Molecular Biology J. MOL. BIOL., vol. 199, no. 4, pp. 649-661, 1988【Abstract】 The crystal structure of manganese superoxide dismutase (MnSOD) from Bacillus stearothermophilus has been solved at 2 multiplied by 4 angstrom resolution by a combination of multiple isomorphous replacement and molecular replacement (1 angstrom = 0 multiplied by 1 nm). The structure has been refined to a conventional R-factor for all 16,560 unique reflections at 2 multiplied by 4 angstrom of 0 multiplied by 26, and the 2F sub(o)-F sub(c) density maps show features more consistent with the known amino acid sequence of MnSOD from B. stearothermophilus) than with the starting model, the MnSOD from Thermus thermophilus.13Direct sequencing of superoxide dismutase genes from two bacterial strains Bacillus circulans and Aerobacter aerogenes amplified by polymerase chain reaction. / Lee,-S.O.; Kim,-S.W.; Uno,-I.; Lee,-T.H. / Bioscience,-Biotechnology,-and-Biochemistry (Japan). (Sep 1993). v. 57(9) p. 1454-1460.专利1Bacillus licheniformis bacterial strain and its micro ecological preparation Inventor: WU TIELIN (CN) Applicant: WU TIELIN (CN) EC: IPC: C12N1/20 ; A61K35/74 Publication info: CN1178833 - 1998-04-15 【abstract】 The said microecological preparation has high cure effect on acute and chronic enteritis, minor dysentery and bacterial vagia disease. The said preparation is relatively stable and this can simplify storage condition for long period storage.2 Micro ecological preparation of bacillus subtilis extract Inventor: FUTIAN LI (CN); JIE SUN (CN); (+1) Applicant: YAN SHUANGQIANG (CN) EC: IPC: A61K35/74 Publication info: CN1136437 - 1996-11-27【abstract】 The invented microecological preparation contains1-2kg of 0.1-10% water solution of active component extract of bacillus substilis and corresponding weight portion of white sugar, brown sugar, honey and extract of fruit of Chinese wolfberry. The extract of stock solution is undergone through a series of specially designated operation process. Its advantages are long expiry period, lenient condition of preserving, safety, reliable, and convenient for use.3Pharmaceutical compositions containing superoxide dismutase from Bacillus Stearothermophilus and Bacillus Caldotenax Inventor: BOWN KEVIN JOHN (GB); BREHM JOHN KARL (GB); (+3) Applicant: HEALTH LAB SERVICE BOARD (GB) EC: C12N9/02M IPC: A61K38/44 ; C12N9/08 ; (+1) Publication info: US5772996 - 1998-06-30 【abstract】A pharmaceutical composition for use in the prophylaxis or treatment of pathological conditions resulting from the presence of superoxide radicals, comprising a manganese-superoxide dismutase (MnSOD) enzyme and a pharmaceutically acceptable excipient. The MnSOD enzyme is in native form and has substantially the amino acid sequence of SEQ ID NO: 17 or SEQ ID NO: 29 and is free of pyrogens consisting of macromolecular substances native to Bacillus Stearothermophilus (BS) or Bacillus Caldotenax (BC). Processes for producing the novel pharmaceutical composition and a method for the prophylaxis or treatment of pathological conditions resulting from the presence of superoxide radicals utilizing the novel composition are also disclosed.4process for producing enzymes having superoxide dismutase activity, novel superoxide dismutase enzymes and novel pharmaceutical compositions comprising enzymes having superoxide dismutase activityInventor: BOWN KEVIN J (GB); BREHM JOHN K (GB); (+3) Applicant: HEALTH LAB SERVICE BOARD (GB) EC: IPC: C12N15/53 ; C12N9/02 ; (+2) Publication info: CA2088574 - 1992-02-04 【abstract】2088574 9202625 PCTABS00010 The genes (sod) encoding Bacillus stearothermophilus and Bacillus caldotenax Mn-superoxide dismutase have been cloned in Escherichia coli and their entire nucleotide sequences determined. With the exception of the post-translationally cleaved N-terminal methionine residue, the predicted amino acid sequence of the B. stearothermophilus enzyme (BSMnSOD) exhibits 100 % similarity to the previously determined amino acid sequence. The B. caldoenax enzyme (BCMnSOD) differs by two amino acids from BSMnSOD. Both recombinant MnSODs were shown to be functionally active in E. coli, both in vitro and in vivo, and were expressed to 47 % of the cells soluble protein by coupling their transcription to the E. coli trp promoter. The pharmaceutical use of these bacterial superoxide dismutases is described.5Solid fermentation process of producing waxy microecological bacillus prepn Inventor: CHEN HONGZHANG (CN); LI ZUOHU (CN); (+1)Applicant: INST OF CHEMICAL METALLURG CHI (CN) EC: IPC: A61K39/02 ; C12N1/20 Publication info: CN1332014 - 2002-01-23【abstract】 The present invention relates to solid fermentation producing process of microecological preparation of waxy Bacillus cereus DM423 and fe