甲胎蛋白(AFP)ELISA试剂盒说明书.docx

甲胎蛋白(AFP)ELISA试剂盒说明书 甲胎蛋白(AFP)ELISA试剂盒说明书Elisa kit规格：48孔配置/96孔配置标准品稀释液：1.5ml1瓶酶标试剂：3 ml1瓶（48）/6 ml1瓶（96）用途：科研实验等领域科学研究，不得用于临床诊断。供应商：上海樊克生物有限公司甲胎蛋白(AFP)ELISA试剂盒说明书甲胎蛋白(AFP)ELISA试剂盒说明书试剂盒操作步骤：温育：用封板膜封板后置37温育30分钟。配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用。洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。加酶：每孔加入酶标试剂50l，空白孔除外。试剂盒样品的采集和存储血清-使用血清分离管，使样品在室温下或凝块一夜之间两个小时在大约10004oC G.测定新鲜制备的血清离心20分钟立即或存放样品在-20C或稀释后使用80oC。避免反复冻融循环。血浆采集血浆用EDTA或肝素作为抗凝剂。15分钟，离心样品G在2到8摄氏度范围内采集30分钟。去除血浆和即刻检测或者储存样品液体类标本：包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。）血清：室温血液自然凝固10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。）血浆：应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。）尿液：用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照此实行。）细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/ 分）。仔细收集上清。检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并 放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。）组织标本：切割标本后，称取重量。加入一定量的PBS，PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8的温度。加入一定量的PBS（PH7.4），用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清。分装后一份待检测，其余冷冻备用。甲胎蛋白(AFP)ELISA试剂盒说明书Operational matters needing attention:The reagent should label storage instructions, return to room temperature before use. After thinning of the standard products should be discarded, can not be saved.No - in the experiment of the plate should be immediately put back the bag, sealed to prevent spoilage.- no other reagents should be packaged or covered. Do not mix different batches of reagents. Before use. using disposable suction head to avoid cross contamination and draw the termination of the liquid and the substrate of a and B liquid, avoid using a portion of the metal sample adding device.Plastic container configuration - use clean washing liquid. All samples of ingredients and mix well before use in the kit.The A substrate should be volatile, avoid long time to open the lid. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid hand contact, toxic. After completion of the experiment should be immediately read od.The addition of reagents should be consistent in order to ensure that all reaction plate hole incubation time.The temperature in accordance with the amount of liquid and the sequence of instructions indicated time, sterile operation. 甲胎蛋白(AFP)ELISA试剂盒说明书樊克生物专业经营进口分装和原装、国产/进口Elisa试剂盒，96T/48TElisa试剂盒，96T/48Telisakit价格，国产/进口血清，生化试剂，标准品|对照品、培养基等科研生化试剂，品质保证，技术严格，无效果退款退货，欢迎来电咨询及订购。友情提醒：公司经营的产品均为科研实验用，不可用于临床应用。 甲胎蛋白(AFP)ELISA试剂盒说明书ELISA Kit1 sensitivity: the minimum concentration is less than 1 standard. Linear dilution. Sample linear regression correlation with expected concentration coefficient R value is 0.990.2: no specific reaction with other cytokines.3 repeatability: plate, plate between the coefficients of variation were less than 10%.The result of judgment and analysis1, the instrument value: Yu Bo 450nm ELISA od read the hole on the instrument2, to the OD value as the ordinate, y, corresponding HLA-B27 standard concentration as the abscissa, x, do the corresponding curve, HLA-B27 content in the sample can be accord