2010-2011分子生物学试卷A马丽1.doc

姓名： 学号： 系： 专业： 年级： 班级： 密 封 线6. To successfully initiate the transcription of mRNA genes by RNA Pol II in vivo, it requires_（ ）ATFIID binds to the TATA box and TFIIB binds to BRE elements of the core promoter.BDifferent s factors CThe inducer complexDReverse transcriptase7. The linear length of most tRNAs is commonly ( ) nt. A76 B85 C140 D208. Which one of the following statements is true?（ ）ATBP binds to TATA box Bthe TATA box has a role in transcription efficiency but not in positioning the start of transcriptionCRNA Pol II only transcribes protein-coding genesDenhancers typically lie 100-200 bps upstream from the start of transcription9. Utraviolet light usually causes mutations by a mechanism involving（ ）Aone-strand breakage in DNA Bdeletion of DNA segments Cinversion of DNA segments Dinduction of thymine dimmers 10. Which best represents the events that occur during translation?（ ）ADNA= mRNA BmRNA=tRNA CrRNA=protein DmRNA=protein 11. The type of DNA found most commonly in living cells is the（ ） form. A B BZ CC DA 12. A tRNA molecule carries the anticodon UGC. The corresponding codon in mRNA is（ ）AUGC BGCA CCGU DAUG13. In the lac operon, the gene that encodes the -galactosidase enzyme is（ ） AlacA BlacY ClacZ DlacO14. The products for RNA polymerase in eukaryote include（ ）得分阅卷人五、 （共 题，每题 分， 共 分）枣庄学院20102011学年第1学期专业课分子生物学导论 考试试卷考试时间： 2011年 月 日 时 分 时 分 试卷类型：A题号一二三四五六七八九十成绩复核签字得分登分签字说明：本试卷分 五 部分，共 100 分考生须知:1.姓名、学号、系、专业、年级、班级必须写在密封线内指定位置。2.答案必须用蓝、黑色钢笔或圆珠笔写在试卷上，字迹要清晰，卷面要整洁，写在草稿纸上的一律无效。得分阅卷人一、单项选择题 （每题选一个最佳答案，共 30 题，每题 1 分，共 30 分）1 Which element is not found in a molecule of DNA? （ ）A carbon B sulfur C nitrogen Doxygen2. How many times is DNA condensed by packing into chromosome in eukaryote？（ ）A6 times B10 times C240 times D10000 times3. （ ）is a component of the ribosomes small subunit of the eukaryote？A16SrRNA B18 SrRNA C5 SrRNA D28SrRNA4. The sequence of nucleotides in one strand of DNA is 3 - GGTACGAT- 5, The corresponding sequence of the mRNA produced from this gene is （ ）? A5-CCATGCTA-3 B3-GGTACGAT-5 C3-ATCGTACC-5 D5-CCAUGCUA-35. Which subunit of the RNA polymerase holoenzyme is essential for recognition of the two consensus sequences in an E.coli promoter?（ ）A-subunit B-subunit Crho factor D sigma() factor 共 页，第 页 共 页，第 页共 8 页，第 1 页 共 8 页，第 2 页ATRNA and 5sRNABmRNA and some snRNAConly rRNA D. many RNAs 15Genes located in which region of a eukaryotic chromosome are most likely to be transcribed?（ ）Acentromere Btelomere Ceuchromatin Dheterochromatin 16. Griffith injected mice with different types of bacteria. For each of the following bacteria types injected, indicate whether the mice lived or died: 格里菲斯将不同类型的细菌注射到老鼠体内，请根据注射的细菌类型，判断老鼠是存活还是死亡？（ ）a. R strain b. S strain c. heat-killed S strain d. R + heat-killed S Alived lived died diedBdied lived died lived Clived died lived died & p) w9 a- 0 k# ?: D/ xDdied died lived lived 17In prokaryotes, the upstream mRNA sequence required for the initiation of protein synthesis is the () sequenceAPribnow BHogness CWatson-Crick DShine-Dalgarno 18. Which is a characteristic of a rho () -independent terminator?（ ） Arho proteinBhairpin loop followed by a string of U.Chairpin loop without a string of U. Da string of U without a hairpin loop.19Dicer protein is（ ）Atranscriptional factor B regulating factor for DNA replicationCnucleases Da RNase for cleaving dsRNA in RNAi20. Which event occurs at the termination of translation?（ ）AmRNA binds to a ribosome BtRNA binds to an amino acid CrRNA dissociates from a ribosome DtRNA dissociates from a ribosome21. In agarose gel electrophoresis(琼脂糖凝胶电泳)，（ ）A DNA migrates towards the negative electrode Bethidium bromide （EB）can be used to visualize the DNAClarger molecules migrate faster than smaller moleculesDsupercoiled plasmids migrate slower than their nicked counterparts 22. Transcription and translation are coupled in （ ）.Ayeast Bhuman Cbacteria Dprotozoa 23. Which type of RNA is involved in the splicing of pre-mRNA molecules in eukaryotic cells?( )ArRNA BhnRNA CsnRNA DgRNA24. In E. coli, the effector molecule that results in induction of the lac operon is（ ）A. allolactose B. catabolite activator protein C. cyclic AMP D. -galactosidase.25. When anti-codon pairs with codon, the wobble base generally refers（ ）Athe first base of anti-codon Bthe third base of anti-codon Cthe first base of codon Dnone of above26. The DNA and histone proteins in a eukaryotic chromosome are compacted into structures called（ ）Aproteosomes Bnucleosomes Ctelomeres. Dcentromeres27. The catabolite activator protein (CAP) binds to the _ to regulate expression of the lac operon（ ）Apromoter Bcyclic AMP Crepressor Dinducer28. When cultures of E. coli are grown in the presence of lactose and glucose, the lac operon is under（ ） control. Apositive Bnegative Cboth positive and negative Dneither positive or negative 29. Which of the following is an example of a nonsense mutation?（ ）AACG to ACC BAUG to UUG CUAC to UAG DAAA to UUU30. For the gene expression of eukaryote, which element promotes the transcriptional efficiency?（ ）Aattenuator Benhancer Crepressor DTATAbox得分阅卷人十、 （共 题，每题 分， 共 分）密 封 线共 8 页，第 3 页 共 8 页，第 4 页得分阅卷人二、填空题（共20个空，每个空0.5分，共10分）1. DNA复制时，随着复制叉移动会产生_，拓扑异构酶II通过引入_来消除复制叉前进时产生的扭曲张力。 2. 大肠杆菌DNA复制有三种相关的酶，分别为DNA聚合酶、DNA聚合酶、DNA聚合酶，其中起主导作用的酶是_。3. 大肠杆菌RNA聚合酶由核心酶）和（）因子组成, 其中前者由_亚基、_亚基和_亚基组成。4. 乳糖操纵子操纵子的天然诱导物是_ ， 实验室里常_作为乳糖操纵子的安慰诱导物诱导-半乳糖苷酶的产生。5在锥虫的线粒体中，RNA编辑是由_指导进行，通过与编辑前mRNA上被编辑区域相邻的碱基互补确定被编辑位点。6. 氨酰-tRNA合成酶对_和相应的_有高度的选择性。7. 利福平是原核生物RNA聚合酶抑制剂，其原因是RNA聚合酶的_亚基对利福平敏感。8. 蛋白质合成中，肽酰转移酶催化 _位的肽酰tRNA的氨基酸和 _位的氨酰tRNA的氨基酸形成肽键。9. 色氨酸操纵子的调控基因 _ 表达的阻遏蛋白与操纵基因结合后可调控结构基因的转录。10. 端粒酶由_和蛋白质两个部分组成，它的生理功能是维持端粒的完整。11. 真核生物RNA 聚合酶的转录因子TFH具有多种酶活性，利用激酶功能可使_的 CTD 磷酸化，在启动子清除时利用其_活性使DNA解旋开始RNA转录。12真核生物中，转录因子与DNA发生特异性结合的结构域种类有HLH、_、_和同源异型域。得分阅卷人三、名词解释（共10题，每题2分，共20分）1. Operon2. Reverse genetics3.Transacting factor4. Signal peptide5. Open reading frame(ORF)6. Promoter7. Polycistronic mRNA