结核病潜伏期

流式网络课堂第五课,流式新闻,苏州跃亚技术部 孟琳 2011-11-30,利用CD4+TNF-a+细胞群体鉴别结核病的潜伏期和活动期,latent Mtb infection,active tuberculosis disease,The frequency of single-positive TNF-producing CD4+ T cells was higher in individuals with active disease,the cutoff of 37.4% of single-positive TNF-,结论： The proportion of TNF- single-positive Mtb-specific CD4+ T cells, and particularly the cutoff at 37.4%, could discriminate between latent infection and active disease. The sensitivity and specificity of the flow cytometrybased assay were 67% and 92%, respectively, the positive predictive value was 80% and the negative predictive value was 92.4%. The proportion of single-positive TNF-a Mtb-specific CD4+ T cells is a new tool for the rapid diagnosis of active tuberculosis disease.,Th17细胞在乳腺癌组织中的表达及与临床参数的关系,We observed that expression of Th17 cells was signficantly higher in breastcancer compared to normal breast tissue.,A :In cancer tissue.,B: In normal tissue,In cancer tissue, expressions of IL-17, IL-1b, and IL-6 positively correlated with expression of Th17 ; Expression ofTh17 was not associated with expression of Treg.,Th17细胞数与肿瘤临床分期，血管浸润和淋巴结转移数呈负相关,结论： Th17细胞参与抗肿瘤免疫应答，并且可能成为未来免疫治疗预后的检测指标。,肿瘤抑制基因RUNX3在骨髓巨细胞瘤中的作用,GCTB病人体内RUNX3的表达率明显低于正常骨髓组织,上图为RUNX3 mRNA在正常人（N）和肿瘤患者（C）中的水平,下图为RUNX3蛋白的表达。,体外表达的RUNX3强烈抑制GCTB细胞的生长，诱导细胞凋亡,结论： 1 本文首次确定RUNX3在GCTB中作为抑制基因。 2 RUNX3的作用提高了GCTB的诊断和治疗。 3 在GCTB 中RUNX3 的信号通路仍不是很清楚，需要进一步研究。,利用流式细胞术定量分析型胶原的 缺失,UCMD（先天性肌营养不良症）组,未破膜处理,破膜处理,对照组,BM（贝特伦肌病）组,型胶原蛋白在成纤维细胞中的表达（细胞膜p 0.001和胞浆内）,（19.6 10.0）%,（65.3 10.7）%,（54.1 8.0）%,型胶原蛋白在胞浆内表达量（p 0.001)）,30 10.3%,70.9 7.5%,42.0 8.5%,结论 ： 和对照组相比，在UCMD病例中型胶原至少降低60%，在BM病例中，平均下降20%。 流式技术定量分析VI型胶原的缺失对我们以后研究基因变异个体具有重要的未知意义。,脊椎关