血液细胞分析仪原理及应用

Principle and application of blood cell analyzer (血液细胞分析仪原理及应用),Human physiological and pathological changes, often caused by changes in blood components, so that a timely understanding of the changes in the blood component, can provide a diagnosis and treatment of diseases of the important basis for doctors.,Red blood cellBiconcave disc SeedlessRed blood cell diameter between 6 9 m, an average of 7.2 M.The number of red blood cells of the adult male was (4.6 6.2) * 1012 /LThe number of red blood cells of the adult female was (5.4 4.2) * 1012 /L,White blood cellNuclear sphereThe diameter is 7 25 M.The number of white blood cells in normal persons was (10 4) * 109 /L,PlateletBoth sides of the micro concave disk, when stimulated pseudopodiaThe normal platelet value (100 300) * 109 /L, diameter 2 4 m.,Hemoglobin (hemoglobin or heme)Hemoglobin is present in red blood cells and is a kind of protein containing iron.Adult male is 120 160g/LAdult female is 110 150g/L,Measurement parameters of blood cell analyzer,Red blood cell RBCWhite blood cell WBCPlatelet PLTHemoglobin HGB,Red blood cell RBCWhite blood cell WBCPlatelet PLTHemoglobin HGB,一、The development history of blood cell analyzer,In 1956, the American scientist W.H.Coulter patented technology for the use of the resistance method to count the number of particles in the blood cell count to be successful.The principle is based on the nature of the non conduction of blood cells, the blood cell count and the volume of blood cells were detected by the blood cell count and volume measurement. The method is called the resistance method or the Kurt principle.,国外厂商：日本SYSMEX美国beckman-coulter日本KOHDEN美国DANAM瑞典BOULE美国BAYER美国ABBOTT,国内厂商：南昌特康南昌百特深圳迈瑞桂林百利特,1. 库尔特计数原理,库尔特原理（coulter principle） ：,二、血细胞计数检测原理,Coulter principle,When the electrolyte is suspended in the electrolyte, the same volume of electrolyte is replaced by the electrolyte through the hole, and the electric resistance of the two electrode is changed, and the potential pulse is generated. The size and frequency of the pulse signal is proportional to the size of the particles and the number of the particles.,二、血细胞计数检测原理,仪器是如何进行细胞分群的呢？,仪器除给出细胞数据结果外，同时提供出细胞体积分布图形，这些可以表示出细胞群体分布情况的图形被称为直方图它可以显示出某一特定细胞群的平均细胞体积、细胞分布情况和是否存在明显的异常细胞群直方图是由测量通过感应区的每个细胞脉冲累积得到的，根据库尔特原理可以在计数的同时进行分析测量。,二、血细胞计数检测原理,Relationship between histogram and pulse signal,二、血细胞计数检测原理,三、Structure of ACT diff Coulter blood cell analyzer,The volume and number of blood cells were measured by using the principle of Kurt resistance, and the hemoglobin was detected by using the principle of monochromatic light color, and the three classification of white blood cells was obtained according to the distribution of blood cell volume.。35 to 50 samples per hour. WBC,RBC,HGB,PLT,LY,MO,GI,三、Coulter ACT diff血细胞分析仪的结构,Card slotCards and reagents for the corresponding batch should be,三、Coulter ACT diff血细胞分析仪的结构,Counting software card,Positive internalSuction needleReaction tankPhotoelectric tubeSolenoid valveScan tubeVacuum isolation chamber,三、Coulter ACT diff血细胞分析仪的结构,Reaction tank,三、Coulter ACT diff血细胞分析仪的结构,Right sideCreeping pumpDilution liquid storage tankNeedleSolenoid valveFilterAir pump,三、Coulter ACT diff血细胞分析仪的结构,Pinch valve,三、Coulter ACT diff血细胞分析仪的结构,Valve core,Control board,三、Coulter ACT diff血细胞分析仪的结构,Instrument structureGas path: vacuum - pumping waste liquid, drying; compressed air - producing bubblesLiquid path: sample, hemolytic agent, diluent, waste liquid and cleaning liquidCircuit: control of gas path, counting and calculation.,三、Coulter ACT diff血细胞分析仪的结构,Piping working process:SampleWipe the needle into the external and drySample dilution such as WBC reaction tankThe two dilution sample was injected into the RBC reaction tankIn WBC reaction tank, the injection of hemolytic agentWBC count (control), PLT count, HGB count (photoelectri