多糖论文：迭鞘石斛所含多糖对小鼠免疫细胞影响的研究.doc

多糖论文：迭鞘石斛所含多糖对小鼠免疫细胞影响的研究【中文摘要】石斛是我国常用的贵重药材,它具有益胃生津、滋阴清热、润肺止咳等功效。现代药理学研究发现,石斛不但具有治疗白内障、降血糖、增强人体免疫力的功能,还具有抗肿瘤、抗氧化、抗衰老和抗血栓等作用。石斛多糖作为石斛属植物重要的药用成分之一,已被证实具有增强免疫力的作用。为进一步探讨和研究石斛多糖对免疫力提高的作用机制,本研究采用正常小鼠免疫细胞体外培养的方法,通过添加提取自迭鞘石斛(Dendrobinm chryseum Rolfe.)的多糖成分,并给予LPS和ConA刺激,观察这类多糖对小鼠脾细胞和小鼠腹腔巨噬细胞增殖作用的影响以及对细胞因子的影响。(1)研究迭鞘石斛所含多糖对小鼠脾淋巴细胞以及腹腔巨噬细胞增殖的影响；(2)研究迭鞘石斛所含多糖对小鼠细胞因子的影响。方法：(1)分离提取小鼠脾淋巴细胞和腹腔巨噬细胞,通过添加和不添加迭鞘石斛所含多糖进行体外培养,以MTT法检测这些免疫细胞增殖的变化情况。(2)分离提取小鼠脾淋巴细胞和腹腔巨噬细胞,通过添加和不添加迭鞘石斛所含多糖进行体外培养,以ELISA法检测培养液中所含IL-2、IL-6的含量。结果：(1)迭鞘石斛所含多糖在体外对小鼠脾淋巴细胞增殖以及腹腔巨噬细胞的增殖有增强作用,说明对小鼠具有免疫增强作用。(2)通过迭鞘石斛多糖对小鼠细胞因子影响的实验发现,迭鞘石斛多糖对小鼠脾淋巴细胞在ConA刺激下分泌IL-2具有明显促进作用(p0.01),并且具有量效关系；对小鼠腹腔巨噬细胞在LPS刺激下分泌IL-6也具有明显的促进作用(p0.01),并且具有量效关系。结论：(1)通过迭鞘石斛多糖对小鼠免疫细胞增殖的实验发现,迭鞘石斛多糖对于小鼠脾淋巴细胞以及腹腔巨噬细胞都有一定的促进作用,低浓度时,作用不显著(2)通过迭鞘石斛多糖对小鼠细胞因子影响的实验发现,迭鞘石斛多糖对小鼠脾淋巴细胞在ConA刺激下分泌IL-2具有促进作用,并且具有量效关系；对小鼠腹腔巨噬细胞在LPS刺激下分泌IL-6也具有促进作用,并且具有量效关系。【英文摘要】Dendrobium as an expensive medicinal herb, which has many important effects, has been widely used in China. Modern pharmacology study showed that Dendrobium has many therapeutic effects, such as improving cataract, antitumor, enhancing immunity, reducing blood sugar, antioxidation, antiaging and antithrombotic. Polysaccharides from Dendrobinm as one kind of important effective components in Dendrobinm has been proven to be able to enhance immunity. In order to further study the action mechanism of Dendrobinm polysaccharides on immunity, this study investigated the effects of polysaccharides from Dendrobinm chryseum Rolfe on growthing of mice spleen cells and peritoneal macrophages stimulated by LPS and ConA with MTT, and on expression of some cytokines of the cells with ELISA.:(1) To study the effects of the polysaccharides extracted from Dendrobinm chryseum Rolfe on proliferation of mice spleen lymphocytes and peritoneal macrophages. (2) To research the influences of the polysaccharides extracted from Dendrobinm chryseum Rolfe on IL-2, IL-6 of mice spleen lymphocyte and peritoneal macrophages.Method:(1) The mice spleen cells and peritoneal macrophages were cultured in vitro. They were stimulated by LPS and ConA. They were treated with or without the polysaccharide. Their proliferations were detected with MTT. (2) The mice spleen cells and peritoneal macrophages were cultured in vitro. They were treated with or without the polysaccharide. Their IL-2, IL-6 of the culture medium were detected with ELISA.Conclusion:(1) The polysaccharide from Dendrobinm chryseum Rolfe can promote the proliferation of mice spleen lymphocytes and peritoneal macrophages in vitro (p0.01). It is able to enhance the immunity of mouse. (2) The polysaccharide from Dendrobinm chryseum Rolfe can promote the expression of IL-2, IL-6 of spleen lymphocytes and peritoneal macrophages stimulated by LPS or ConA (p0.01). These effects had the concentration-response relations.【关键词】多糖 脾淋巴细胞 腹腔巨噬细胞 迭鞘石解 细胞因子【英文关键词】Polysaccharide Spleen lymphocyte Peritoneal macrophage Dendrobinm chryseum Rolfe Cytokine【目录】迭鞘石斛所含多糖对小鼠免疫细胞影响的研究摘要6-7Abstract7第1章 绪论10-291.1 石斛10-151.1.1 石斛简介101.1.2 常见的石斛种类10-131.1.3 石斛的主要药用成分13-141.1.4 石斛的药用价值14-151.2 石斛多糖15-171.2.1 石斛多糖的提取工艺15-161.2.2 多糖的结构16-171.3 石斛多糖的药理学研究17-191.3.1 抗肿瘤作用171.3.2 增强免疫系统作用17-191.3.3 降血糖作用191.4 迭鞘石斛19-211.4.1 迭鞘石斛的分布191.4.2 迭鞘石斛的鉴定19-201.4.3 迭鞘石斛的化学成分20-211.5 迭鞘石斛药理作用21-221.6 免疫细胞22-241.6.1 巨噬细胞221.6.2 淋巴细胞22-241.7 细胞因子24-291.7.1 白细胞介素25-261.7.2 集落刺激因子26-271.7.3 干扰素271.7.4 肿瘤坏死因子27-281.7.5 淋巴因子281.7.6 单核因子28-29第2章 迭鞘石斛多糖对小鼠免疫细胞增殖的影响29-442.1 迭鞘石斛多糖对小鼠脾淋巴细胞的影响29-372.1.1 实验材料29-302.1.2 实验步骤30-362.1.3 实验结果36-372.2 石斛多糖对小鼠腹腔巨噬细胞的影响37-422.2.1 实验材料37-392.2.2 实验步骤39-402.2.3 实验结果40-422.3 讨论42-44第3章 迭鞘石斛多糖对小鼠细胞因子