2003年上海交大生化期末试卷1※2

上海交通大学生物化学试卷（2003年度）校名_ 系名_ 专业_姓名_ 学号_ 日期_2003 TEST PAPER (1)（闭卷部分）(Shanghai Jiao Tong University,1st term of Univ. year 2003)Subject: Biochemistry Name _ Score _ Class _一. Explain the terms:（2.5 x 10= 25）1. hyperuricemia 2. LDL receptor 3. pyruvate dehydrogenase complex 4. glucokinase (in liver) 5. malate-aspartate shuttle 6. PRPP 7. substrate cycle 8. “macro CoA” 9. urea cycle 10. UDP- glucose 2003 TEST PAPER (2)（开卷）(Shanghai Jiao Tong University,1st term of Univ. year 2003)Subject: Biochemistry Name _ Score _ Class _二. Filling the blanks: (2.5 x 12= 30)1. Many important coenzymes, such as_, _and _, are the derivatives of ADP; dNDP are synthesized from _ and dTMP is formed by_ of dUMP; all these facts reflects that_ came before_ in evolution.2. TPP is the derivative of vitamin _, it serves as the prosthetic group of the following enzymes: _, _and_.3. There is a large difference between the yield from the complete oxidation of triacylglycerols and of carbohydrates (and protein), the reason is _.4. The three pairs of enzymes that catalyze the three resemble steps in -oxidation of FA and citric acid cycle are_ and_, _and_, _and_. 5. The only reactions in mammals that require coenzyme B12 are_ and _ catalyzed by _ and _, respectively.6. OAA has several possible fates: _, _, _ and _.7. In the synthesis of phosphoglycerides磷酸甘油酯 and of glycogen, the activated intermediates are _ and_, both of them are formed from a _ substrate and a _, and then react with a _ group.8. The committed step in cholesterol biosynthesis is the synthesis of _ from _which is catalyzed by _.9. The carrier of the activated one-carbon units is_ which consists of three groups: _, _ and_. Besides, _ is the major donor of methyl group. The fully oxidized one-carbon unit is _, which is carried by_.10. Disease and inherited defects of enzyme or functional protein, e.g. PKUPhe hydroxylase; drug-induced hemolytic anemia溶血性贫血_; hypercholesterolemia高胆固醇血症_; pernicious anemia恶性贫血_; maple syrup槭糖浆 urine disease_; Lebers optic neuropathy视神经症 _.11. Acetyl CoA carboxylase from E. coli consists of three subunits called _, _ and _.12. There are two types of covalent modification: _ and_in the regulation of enzymatic activity, the typical example of which is_ and _(enzyme), the direct related residue is_ and _, respectively. 三Make choice (one or several)：(3 x 8= 24)1. Schiff base intermediates are formed in the reactions catalyzed byA. aldolase B. glycogen phosphorylase C. Trp synthetase D. transaldolase E. aminotransferase2. Multifunctional (or bifunctional) enzyme occurs inA. pyrimidine synthesis of eukaryotes b. FA synthesis of eukaryotes C. the synthesis and hydrolysis of F-2,6-BP D. the phosphorylation and dephosphorylation of isocitrate DH E. FA synthesis of E. coli3. PRPP participates in the synthesis of the following substanceA. Pro B. His C. purine D. Phe E. pyrimidine4. Biotin, as the posthetic group of the following enzymeA. acetyl CoA carboxylase B. transketolase C. pyruvate carboxylase D. Pyr DH E. KG DH5. The following complex contains Fe-S clusterA. NADH-Q reductase B. cytochrome c reductase C. cytochrome c oxidase D. succinate-Q reductase E. ribonucleotide reductase6. Which substance is the direct precursor of the synthesis of acetoacetate乙酰 乙酸 in liverA. HMG CoA B. acetoacetyl CoA C. D-3-hydroxybutyrate羟丁酸 D. D-3-hydroxybutyryl CoA E. acetone7. Citric acid cycle A. it can produce high-energy phosphoryl compound by itself B. it is not inhibited by the anaerobic厌氧 condition C. the two carbon atoms of acetyl CoA are liberated as the form of two CO2 in that round of the cycle D. its rate depends upon the need of ATP E. it is the final common pathway for the oxidation of fuel molecules.8. It participates in the activated methyl cycle (or S-A Met cycle)A. VB12 B. biotin C. FH4 D. VB2 E. nicotinate烟酸四. Answer or calculate: (7x 3= 21)1. What is the activated reactant in each of these biosyntheses?a) Sphingomyelin鞘磷脂 from ceramide神经酰胺b) Met from homocysteine高半胱氨酸c) Phosphatidyl choline磷脂酰胆碱 from phosphatidyl ethanolamine乙醇胺d) IPP from 5-pyrophosphomevalonate焦磷酸甲羟戊酸e) The synthesis of phosphoribosylamine磷酸核糖胺f) Ser from Glyg) Glycogen n+1 from glycogen n 2. In purine biosynthesis, Asp becomes linked to the carboxylate group of an intermediate. In the next reaction, the carbon skeleton of the Asp unit leaves in the form of fumurate延胡索酸. These reactions are reminiscent重演 of a similar pair of reactions in the degradation of AA. Which ones? 3. Currency exchange. For a proton-motive force of 0.2V (matrix negative), what is the maximum ATP/ADPPi ratio compatible with ATP synthesis? Calculate this ratio three times, assuming that the number of protons translocated per ATP formed is 2, 3, and 4 and that the temperature is 250C.2003 TEST PAPER (1) 答案一.1.hyperuricemia 高尿酸血 血液中存在异常高含量的尿酸，可能由嘌呤合成增加引起。 高尿酸血与痛风症相关。 2.LDL receptor低密度脂蛋白受体 一种与载脂蛋白B相结合的细胞表面受体。可使LDL从血浆(发生细胞)内化，以调节胆固醇和LDL的合成从而调节血浆胆固醇的浓度。 3.pyruvate dehydrogenase complex 丙酮酸脱氢酶复合体? 催化丙酮酸、NAD+和辅酶A之间反应形成乙酰辅酶A、NADH和CO2(整个反应)的酶复合体。包含三个活性酶和五个辅酶。 4.glucokinase (in liver) 葡萄糖激酶 哺乳动物肝赃中特有的一种己糖激酶的同工酶,它与底物葡萄糖的亲和力很底，故只有当血液中葡萄糖浓度很高时它才能与之反应；同时，它不受高水平G-6-P的抑制，故可大量地吸纳血液中(过多)的葡萄糖。 5.malate-aspartate shuttle 苹果酸-天冬氨酸穿梭 可将还原型对应物(NADH中的电子)从从肝脏等组织的细胞质转运到线粒体内。 6.PRPP 磷酸核糖焦磷 生物合成多种含氮环状化合物极其衍生物中必不可少的反应物，特别在合成组氨酸、核苷酸中的嘌呤和嘧啶时。 7.substrate cycle底物循环 反应物和产物在两个酶的作用下的互变，如:F-6-P与F-1,6-P的互变。它的存在提高了调控的敏感性. 8.“macro CoA” 巨”辅酶A”; 指ACP(酰基载体蛋白),它含有与辅酶A相同的磷酸泛酰巯基乙胺头部(与脂肪酸合成过程中各中间物连接);与此部分相连接的是”巨大”的由77个氨基酸残基构成的一条多肽链。 9.urea cycle 尿素循环; 一组循环反应, 其中由氨基酸代谢产物两个氨基和一个二氧化碳生成一个尿素排出体外。 10UDP- glucose 尿苷二磷酸葡萄糖; 一种核苷二磷酸糖,作为糖原合成(和其它许多合成)反应中葡萄糖基的供体。 2003 TEST PAPER (2) 答案 二.1.NAD+，FAD，CoA, NDP, methylation, RNA, DNA 2.B1, pyruvate DH complex, -KG DH complex, transketolase 3.高度还原，不含水 4.酮脂酰-ACP还原酶, 琥珀酸DH; 羟脂酰-ACP脱水酶, 延胡索酸酶; 烯脂酰-ACP还原酶, 苹果酸DH 5.高半胱氨酸甲基化形成甲硫氨酸, L-甲基丙二酰CoA分子内重排生成琥珀酰CoA, 高半胱氨酸甲基转移酶, 甲基丙二酰CoA变位酶 6.柠檬酸循环