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中西医结合学报2011年2月第9卷第2期JOURNALOFCHINESEINTEGRATIVEMEDICINE,FEBRUARY2011,VO19,NO2,一IIETTECTSOTVLTAMLNI0NMERCURICCNL0RIDELNAUCEARENAIINTERSTITIAIFIBROSISINRATSANDTHEANTI0XIDATIVEMECHANISMYANYANTAO,QING1ANWANG,JILIYUAN,LISHEN,CHENGHAILIU。1INSTITUTEOFLIVERDISEASES,SHUGUANGHOSPITAL,SHANGHAIUNIVERSITYOFTRADITIONA1CHINESEMEDICINE,SHANGHAI2012O3,CHINA2KEYLABORATORYOFIIVERANDKIDNEYDISEASESOFMINISTRYOFEDUCATION,SHANGHAIUNIVERSITYOFTRADITIONA1CHINESEMEDICINESHANGHAI2012O3CHINA3EINSTITUTEOFINTERNALMEDICINEOFTRADITIONALCHINESEMEDICINE,SHANGHAIUNIVERSITYOFTRADITIONALCHINESEMEDICINE,SHANGHAI2012O3,CHINA4SHANGHAIKEYLABORATORYOFTRADITIONALCHINESECLINICALMEDICINE,SHANGHAI201203,CHINAOBJECTIVETOOBSERVELHEEFFECTSOFVITAMINEVITEONMERCURICCHIORIDEHGCI2一INDUCEDRENAIINTERSTITIAIFIBROSISRLFINRATSANDDISCUSSITSANTIOXIDATIVEMECHANISMMETHODSATOTALOF32SPRAGUEDAWIEYRATSWERERANDOMLYASSIGNEDTOTHREEGROUPSNORMALGROUP,MODELGROUPANDVJTEGROUPRLFWASINDUCEDBYORAIADMSTRATIONOFHGCI2ATADOSEOF8MGKGBODYWEIGHTONCEADAYFOR9WEEKSRATSINVITEGROUPWEREADMINISTEREDWITHVITECAPSULEAI10OMGKGBODYWEIGHT,ANDRATSINNORMAIANDMODEIGROUPSWERELREATEDWILHNORMAISALINEALTHEENDOFLHE9LHWEEK,RATSWERESACRIFICEDANDRENALHYDROXYPROIIREHYPCONTENTWASASSAYEDWITHJAMALLSMETHODANDCOLLAGENDEPOSITIONWASVISUAIIZEDBYUSINGHEMATOXYNANDEOSINHE,MASSONSTRICHROMEANDPERIODICACIDS_IVERMETHENAMIREPASMSTAININGTHEACTIVITIESOFSUPEROXIDEDISMULASESODANDGIULATHIONEPEROXIDASEGSHPX,ANDCONTENISOFGIULATHIONEGSHANDMALONDIALDEHYDEMDAINKIDNEYTISSUEWERETESTEDWILHCOMMERCIALKITSTHEEXPRESSIONSOFNUCIEARFACFORICBNFKB,INHIBILORICBJCB,PHOSPHOLICBPIJCBANDLUMORNECROSISFACTORATNFAWEREDETERMINEDBYWESTERNBLOTTHEEXPRESSIONOFASMOOTHMUSCIEACTINASMAWASASSAYEDBYWESTERNBLOTANDIMMUNOFLUORESCENTSTAININGRESULTSRENAIHYPCONTENL,HE,MASSONSIRICHROMEANDPASMSTAININGRESULTSANDASMAEXPRESSIONCONFIRMEDDEVELOPMENTOFHGCI,一INDUCEDRLFINRATSOXIDATIVESTRESSMARKERSGSH。GSHPXANDMDACONFIRMEDOXIDATIVESTRESSINRLFRATSCOMPAREDWITHMODELRATS,RATSDOI103736JCIM20110214姜哲浩,崔红燕,刘成海,刘成,刘平,徐列明,胡义HTTPWWWJCIMJOURNA1CORN扬,袁继丽,李风华扶正化瘀方对大鼠中毒性肾间质TAOYY,WANGQI,YUANJL,SHENL,LIUCHEFFECTSOF纤维化的作用中西医结合学报2004;25358360VITAMINEONMERCURICCHLORIDEINDUCEDRENALINTERSTITIALFIBROSISINRATSANDTHEANTIOXIDATIVEMECHANISMJCHININTEGRMEDJIANGZH,CUIHY,IIUCH,LIUC,LIUP,XULM,2011;92201208HUYY,YUANJL,LIFHEFFECTSOFFUZHENGHUAYU陶艳艳,王清兰,袁继丽,沈丽,刘成海维生素E抑制氯化RECIPEONRENALTOXICINTERSTITIALFIBROSISINRATSJCHIN汞诱导大鼠肾间质纤维化的抗氧化作用机制中西医结合学INTEGRMED2004;25358360FULLTEXTAVAILABLEATHTTPFWWWCIMJOURNA1CORNARTICLES报2O11;92201208PUBLISHARTICLESPDF2006323640998901PDFRECEIVEDSEPTEMBER21,2010;ACCEPTEDNOVEMBER11,2010;PUBLISHEDONLINEFEBRUARY15,2011宦红娣,张景红,郑士荣,孟巍巍,张军力地灵丹汤FULLTEXTLINKOUTATPUBMEDJOURNALTITLEINPUBMEDZHONG对单侧输尿管梗阻大鼠模型肾间质纤维化的防治作用中西医结合学报2008;65493497XIYIJIEHEXUEBAOHUANHD,ZHANGJH,ZHENGSR,MENGWW,ZHANG基金项目新世纪优秀人才支持计划资助项目NONCET一04JLDILINGDANDECOCTIONPREVENTSRENALINTERSTITIALFIBROSIS0437;国家教育部博士点基金资助项目NO20060268005;INARATMODELOFUNILATERALURETERALOBSTRUCTIONJCHIN上海市优秀学科带头人计划资助项目NO08XD14041;上海INTEGRMED2008;65493497高校E一研究院建设计划资助项目NOE03008;上海市高校FULLTEXTAVAILABLEATHTTPWWWJCIMJOURNA1CORNARTICLES创新团队建设计划第一期资助项目PUBLISHARTICLESPDF200851541006PDFCORRESPONDENCECHENGHAILIU,MD,PHD,PROFESSOR;TELMORERELATEDARTICLESATHTTPWWWJCIMJOURNA1COM02120256521;EMAILCHENGHAILIUYAHOOCORNCNENFULLTEXT2ASPXARTICLEIDJCIM20110214202中西医结合学报2011年2月第9卷第2期JOURNALOFCHINESEINTEGRATIVEMEDICINE,FEBRUARY2011,VO19,NO2INVITEGROUPHADIOWERKIDNEYHYPCONTENIP005INVITEGROUP,THEEXPRESSJONSOFPJ,CBANDTNFADECREASEDSIGNIFICANTLYCOMPAREDWJLHMODEJGROUP尸001THEEXPRESSIONOFASMAINVITEGROUPWASALSODECREASEDSIGNIFICANTLYCOMPAREDWJIHMODEIGROUPP001CONCLUSIONVITEHASAPROTECTIVEEFFECLONEXPERIMENTAIRIFINDUCEDBYHGCI2INRATSANDITISRELATEDTOINHIBITIONOFLIPIDPEROXIDATION,WHICHINVOLVESBLOCKINGOFNFJCBSIGNALINGPATHWAYANDTHEACTIVATIONOFCELISPRODUCINGEXTRACEIIUIARMATRIXKEYWORDSVITAMINE;RENALINTERSTITIALFIBROSIS;MERCURICCHLORIDE;LIPIDPEROXIDATION;ANIMAEXPERIMENTATION;RATSAGROWINGBODYOFEVIDENCESUGGESTSTHATMERCURIALCOMPOUNDS,INPARTICULAR,MERCURICCHLORIDEHGCL2ARETOXICTOHUMANS0WINGTOTHEUSEOFMERCURYPESTICIDESINAGRICULTUREORASCOMPONENTSOFBATTERIESORINFLUORESCENTLIGHTBULBS,LOWLEVELSOFMERCURYEXISTSINAWIDEVARIETYOFPHYSICALORCHEMICALSTATESEACHOFWHICHHASUNIQUECHARACTERISTICSFORTARGETORGANSPECIFICITYFOREXAMPLE,EXPOSURETOMERCURYVAPORORTOORGANICMERCURYCOMPOUNDSSPECIFICALLYAFFECTSTHECENTRALNERVOUSSYSTEM,WHILEKIDNEY,LIVERANDGASTROINTESTINALTRACTAREMAINLYTARGETEDBYINORGANICMERCURYCOMPOUNDS,SUCHASHGEL,HGCL,ISONEOFTHEMOSTCOMMONCAUSESOFACUTECHEMICALNEPHROPATHYANDACUTERENAIFAILURE【MERCURYPOISONINGCANINDUCEFIBROGENICGROWTHFACTORSSUCHASTRANSFORMINGGROWTHFACTOR1TGF一1,CONNECTIVETISSUEGROWTHFACTORCTOFANDTUMORNECROSISFACTORATNFAANDACCUMULATIONOFEXTRACELLULARMATRIXECMPROTEINS_DJTHEEXCESSIVEDEPOSITIONOFINTERSTITIA1MATRIXCOMPONENTSWASMAINLYDUETOANEXPANSIONOFINTERSTITIALMVOFIBROBLASTPOPULATIONSTHATARECHARACTERIZEDBYDEGTOVOACTIVATIONOFASMOOTHMUSCLEACTINDSMAPREVIOUSSTUDIESOFRENA1IMPAIRMENTUSUALLYUSEDANIMALMODELOFACUTERENALFAILUREWITHERALORINTRAPERITONEALADMINISTRATIONOFHGC1,IHOWEVERPATHOLOGICALPROCESSOFACUTEMERCURYPOISONINGISDIFFERENTFROM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