4、SCI科研论文英语写作全攻略12节视频pptw 01

酸谈学社 上海尤里卡信息科技有限公司版权所有 英语科研论文写作 第 一 章 总论 Kevin主讲人 课程总览 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 课程总览 1. 英语科研论文写作的一般性指导原则 2. 实验论文的写作 3. 综述的写作 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 课程总览 1. 英语科研论文写作的一般性指导原则 2. 实验论文的写作 3. 综述的写作 写作的三步骤 准备、写作、修改 困扰中国人的一些具体问题及解决方案 语法、句式、时态 漫谈英语学习 - 为科研写作做准备 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 课程总览 1. 英语科研论文写作的一般性指导原则 2. 实验论文的写作 3. 综述的写作 写作的三步骤 准备、写作、修改 困扰中国人的一些具体问题及解决方案 语法、句式、时态 漫谈英语学习 - 为科研写作做准备 Overview 实验论文写作过程概览 Are you ready? 材料收集和准备工作、构思及酝酿 分步写作详解 Materials and methods; Results and discussion; Introduction/background 修改及润色 Revision, polish, and finalization 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 课程总览 1. 英语科研论文写作的一般性指导原则 2. 实验论文的写作 3. 综述的写作 写作的三步骤 准备、写作、修改 困扰中国人的一些具体问题及解决方案 语法、句式、时态 漫谈英语学习 - 为科研写作做准备 Overview 实验论文写作过程概览 Are you ready? 材料收集和准备工作、构思及酝酿 分步写作详解 Materials and methods; Results and discussion; Introduction/background 修改及润色 Revision, polish, and finalization Conceiving and building up your ideas 材料收集和准备工作、构思及酝酿 分步写作详解 Introduction/background; Bullet points; Citing others work; Details (修改及润色 Revision, polish, and finalization) 图和表 professional, presentable, and visually pleasant figures 科研论文的写作流程 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 科研论文的写作流程 1.准备 2.写作 3.修改 文献背景（文献管理软件如Endnote) 前沿动态（构思与自己结果的比较） 结果、图、表 etc. 写的顺序【材料与方法 - 结果 （结论）- 讨论 - 背景】 结论概括 组织结果、图、表 etc. 尽快记录思路，可以只有短句、词组 用时最长 （70%以上时间） 润色定稿 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 Case study 逻辑为王 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 逻辑为王 科研论文行文最重要的就是“逻辑流” logic flows Major point 1 Sub-point 2Sub-point 1Sub-point 3 Evidence1Evidence 2Evidence 3 Conclusion1Conclusion 2 Conclusion 3 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 逻辑为王 常用的行文结构 总-分-总 分-分-总 分-总-分 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 Case study The newly designed microarrays yielded high specificity, sensitivity, and reproducibility in detecting the prevalent carbapenemase genes in the antibiotic-resistant bacterial strains. Unlike the conventional CLSI methods that are disadvantaged by false negative/positives, the microarray method could effectively minimize the false hybridization signals because the technique is based on DNA hybridization process, which is highly specific due to the requirement of perfect base pair matches. On the one hand, our study has shown that the highly specific microarrays can simplify the protocol to determine the cabapenem-resistant genes in the clinical samples, and offer an efficient means for studying the molecular epidemiology of cabapenemase genes the emergence of the resistant genes may potentially be traced back to their origins where community- and hospital-based bacterial infections are frequently happening. As such, we can have insightful understanding on the mechanisms accounting for the proliferation, evolution, and mutagenesis of the resistant genes under antibiotic selections. On the other hand, microarray hybridization is highly sensitive. In this study, we incorporated either Cy3 dye or biotin into the PCR-amplified DNAs that are to be hybridized onto the microarray probes. The fluorescence-based strategy guaranteed the high sensitivity of microarray detection. In our study, we could detect as low as 30 copies/L of DNA targets. Furthermore, the DNA microarrays can generally detect target DNAs with larger dynamic ranges. In our case, we reason that with modifications in hybridization conditions and concentrations of spotted probes, we could further augment the detection range. Finally, the microarray method is also highly reproducible we have shown that the averaged coefficients of variations (CV %) for inter-chip and intra-chip experiments were low, and most of them were less than 10%. Therefore, we propose that the new microarray method has a great potential to be applied to clinical studies. 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 Case study The newly designed microarrays yielded high specificity, sensitivity, and reproducibility in detecting the prevalent carbapenemase genes in the antibiotic-resistant bacterial strains. Unlike the conventional CLSI methods that are disadvantaged by false negative/positives, the microarray method could effectively minimize the false hybridization signals because the technique is based on DNA hybridization process, which is highly specific due to the requirement of perfect base pair matches. 1.On the one hand, our study has shown that the highly specific microarrays can simplify the protocol to determine the cabapenem-resistant genes in the clinical samples, and offer an efficient means for studying the molecular epidemiology of cabapenemase genes the emergence of the resistant genes may potentially be traced back to their origins where community- and hospital-based bacterial infections are frequently happening. As such, we can have insightful understanding on the mechanisms accounting for the proliferation, evolution, and mutagenesis of the resistant genes under antibiotic selections. 2. On the other hand, microarray hybridization is highly sensitive. In this study, we incorporated either Cy3 dye or biotin into the PCR- amplified DNAs that are to be hybridized onto the microarray probes. The fluorescence-based strategy guaranteed the high sensitivity of microarray detection. In our study, we could detect as low as 30 copies/L of DNA targets. 3.Furthermore, the DNA microarrays can generally detect target DNAs with larger dynamic ranges. In our case, we reason that with modifications in hybridization conditions and concentrations of spotted probes, we could further augment the detection range. 4.Finally, the microarray method is also highly reproducible we have shown that the averaged coefficients of variations (CV %) for inter-chip and intra-chip experiments were low, and most of them were less than 10%. Therefore, we propose that the new microarray method has a great potential to be applied to clinical studies. 解 螺 旋|陪 伴 医 生 科 研 成 长 英语科研写作 Case study The newly designed microarrays yielded high specificity, sensitivity, and reproducibility in detecting the prevalent carbapenemase genes in the antibiotic-resistant bacterial strains. Unlike the conventional CLSI methods that are disadvantaged by false negative/positives, the microarray method could effectively minimize the false hybridization signals because the technique is based on DNA hybridization process, which is highly specific due to the requirement of perfect base pair matches. 1.On the one hand, our study has shown that the highly specific microarrays can simplify the protocol to determine the cabapenem-resistant genes in the clinical samples, and offer an efficient means for studying the molecular epidemiology of cabapenemase genes the emergence of the resistant genes may potentially be traced back to their origins where community- and hospital-based bacterial infections are frequently happening. As such, we can have insightful understanding on the mechanisms accounting for the proliferation, evolution, and mutagenesis of the resistant genes under antibiotic selections. 2. On the other hand, microarray hybridization is highly sensitive. In this study, we incorporated either Cy3 dye or biotin into the PCR- amplified DNAs that are to be hybridized onto the microarray probes. The fluorescence-based strategy guaranteed the high sensitivity of microarray detection. In our study, we could detect as low as 30 copies/L of DNA targets. 3.Furthermore, the DNA microarrays can generally detect target DNAs with larger dynamic ranges. In our case, we reason that with modifi