免疫组库测序技术介绍.ppt

Immuno-Seq:exploringthediversityofimmunesystem,JiankuiHeSouthUniversityofScienceandTechnologyofChina(SUSTC),Shenzhen,China,ThediversityofInvaders.,BacteriaVirusesparasitessuchasfungi,protista,5(171):171,接受流感疫苗后,Immuno-Seq,Immuno-Seq技术可以观察和分析T细胞和B细胞，达到前所未有的深度和特异性。在疾病监控，抗体生产，疫苗研究，健康体检等领域有广泛的应用。,现在临床上有许多种检测可以监控你的健康状况，但是却没有一种被广泛接受的方法可以检测你免疫系统的各个方面。我们怎么可以得知一个人的免疫系统是很强健还是很虚弱？在人们受哮喘，过敏，关节炎折磨时，如何量化免疫系统的错误？斯坦福大学的一个研究团队在开发这样的检测方面迈出了第一步，他们的方法是：给免疫系统“拍照”。,Therearenumerousteststogaugethehealthofyourheart.Butnosuchwidelyacceptedtestexistsforthemanypartsoftheimmunesystem.Howcanwetelliftheimmunesystemisstrongorweak?Orquantifyhowbadlyitsmalfunctioningwhenwesufferfromasthma,allergies,orarthritis?,-FrancisCollins,NIHDirector2013年2月13日,Immuneresponsetofluvaccine,Day0,Day7,Day28,FluVaccine:TIV&LAIV,PlasmablastsDay7,Collect5mlblood,Experimentflow,BroadVDJgenesegmentusagepattern,V,D,J,Asnapshotofimmunesystem,Day7,aftervaccination.Elderlysubject,WeamplifiedantibodysequencesfromsinglesortedplasmaBcell.Wethenexpressedmonoclonalantibodiesaccordingtothesesequencesandverifiedtheirbindingtoeachofthethreevirusstrainsinthevaccine,Immunereperotireisadynamicssystem,Day0,Day7,Day28,Vaccinespecificantibodylineage13%oftotalsequences,Vaccinespecificantibodylineage0.01%oftotalsequences,Elderlysubject,PlasmablastsDay7,Reducedantibodydiversityinelderly,Age8-17,Age18-32,Age70-100,Mutation,Eachdatapointrepresentalineage,Comparisonof3subjects,Sequencesinalineage,Reducedantibodydiversityinelderly,70100yearoldvolunteershadasignificantlylessdiversecollectionofantibodiesthantheyoungervolunteers.Thatis,aspeopleaged,theirimmunesystemhadamorelimitedrepertoireofantibodiestooffer,LabMembers,Postdoc:ZhoufangLiTechnician:LuwenNingMengZhangStudents:JiajieGuoYinTongYingXuPengLiYaoChenRonghaiCheng,Collaborators:PinghuaLiu(BostonU),HengyeMan(BostonU)ZhigangLiu(ShenzhenU),ZhanhuiWang(SMU),ZhimingCai(2ndHospitalofShenzhen),XiaoLiu(BGI)XiaoPingChen(CAS),HuaZhang(SMU),Labweb:www.