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HPLC法测定小儿感冒颗粒中绿原酸的含量A1A0A3A4A2A5A6A7A8A9A10A11A12A13A14A15A16A17A18A19A20A21A22A23A24A25A21A26A27A28A29A30A8A31A32A33A19A7A8A34A35A36A37A38A39A40A41A42A43A44A45A46A35,KROMASILC18A47A48A49A50A51A51A52A53A54A50A51A51A55A49MA56A57A58A59A55A60A61A62A63A64A65A66A50A54A67A51A68A69A70A71A72A73A74A75A76A77A78A79A80A81A47A67A82A83A84A53A56A85A60A86A83A67A54A50A51A69A70A51A87A88A85A89A90A91A92A83A93A48A84A88A51A85A59A94A83A48A49A95A96A97A98A99A100A73A101A50A54A67A50A50A50A102A53A54A50A50A50A50GA103A104A105A106A107A108A109A110A111A112A113A114A115A55A116A117A118A119A120A6310039A55RSD101A96A97A121A122A123A124A86A125A126A127A125A128A129A55A130A131A132A133A134A135A136A137A138A139A99A100A73A140A141A142A90A143A96A144A145A146A147A133A134A135A136A137A138A85A99A100A73A85A148A149A81A62DETERMINATIONOFCHLOROGENICACIDINXIAOERGANMAOGRANULESBYHPLCABSTRACTOBJECTIVETODEVELOPAHPLCMETHODFORTHEDETERMINATIONOFCHLOROGENICACIDINXIAOERGANMAOGRANULESMETHODSAHPLCMETHODWASADOPTEDALLOFREFERENCESUBSTANCESANDSAMPLESWERESEPARATEDWITHTHEMOBILEPHASEWHICHISMETHANOL01MOL/LSODIUMDIHYDROGENPHOSPHATE16A15084UNDERISOCRATICELUTIONBYUSINGAKROMASILC18REVERSEDPHASECOLUMNA151250A15240A152A1535MA154A153ANDFLOWRATEWAS10MLMIN1A153THEDETECTIONWAVELENGTHWAS328NMA153THECOLUMNTEMPERATUREWAS25A155RESULTSCHLOROGENICACIDHASALINEARITYINTHERANGEOF01000A15640000GTHEAVERAGERECOVERYOFCHLOROGENICACIDWAS10039,RSD101CONCLUSIONTHISMETHODISSIMPLE,SENITIVE,ACCURATEANDSUITABLEFORQUANTITATIVEDETERMINATIONOFCHLOROGENICACIDINXIAOERGANMAOGRANULESKEYWORDSXIAOERGANMAOGRANULESCHLOROGENICACIDHPLCA157A158A159A160A161A162A163A164A165A166A167A168A169A170A171A172A173A174A174A175A176A177A178A179A180A181A182A183A184A185A186A187A188A189A190A191A192A193A194A192A195A196A192A197A198A199A192A200A201A202A192A203A204A192A203A205A206A192A207A208A192A209A210A192A211A212A213A214A215A216A217A218A219A220A187A221A222A223A224A225A226A187A227A228A225A229A230A231A232A233A234A224A228A235A236A187A237A238A239A228A240A192A241A242A243A192A244A245A246A247A192A248A249A250A243A251A252A235A238A253A254A237A255A92A1A2A3A230A95A0A4A5A193A194A222A6A220A7A8A179A187A221A222A63A9A192A103A10A213A11A231A1A12A3A230A217A13A218A14A173A174A174A175A176A177A178A179A180A181A233A234A235A236A15A16A17A18A217A187A108A222A179A19A20A201A184A209A21A22A23A187A114A24A25A26A27A19A230A119A28A29A30A31A32A33A34A35A128A187A129A36A37A38A39A40A41A31A32A187A136A42A43A44A38A233A234A235A236A15A16A184A140A45A46A47A24A25A26A27A39A40A230A144A48A49A203A50A51A233A234A235A236A15A16A184A52A25A33A53A38A179A186A54A55A192A153A37A192A240A78A56A49A184A52A25A50A51A39A40A2301仪器与材料A57A58A57A59A60A59A60A157A13A158A61A62A64A65A66A67A68A69A69A61A70A45A71A72A174A170A6A73A74A75A76A77A178A176A79A80A81A82A187A47A140A143A62A69A65A46A65A62A64A70A69A182A83A84A185A85A86A87A88A181A251A190A191A89A90A91A93A94A96A47A2A97A7A99A98A178A173A175A174A90A90A200A201A100A174A90A90A187A175A203A90A181A251A158A94A96A90A91A204A101A102A104A178A174A100A201A175A203A90A181A251A208A119A105A106A46A61A142A143A62A143A107A65A109A173A173A174A214A110A111A112A230A57A58A113A115A116A218A117A144A75A76A216A178A157A13A67A94A164A90A91A221A118A187A75A76A231A181A251A223A144A120A51A121A216A223A251A218A117A192A226A122A123A144A7A99A216A230A57A58A124A125A126A127A95A0A4A130A131A185A132A232A217A13A218A185A133A134A51A185A135A27A137A230A57A58A138A139A141A233A234A235A236A15A16A145A185A187A146A237A238A187A239A147A148A238A149A7A23A144A242A176A150A151A152A154A111A51A218A174A71A174A155A174A237A192A176A150A151A152A154A111A51A218A174A71A174A237A174A72A192A176A150A151A152A154A111A51A218A174A71A174A237A155A155A187A123A132A232A189A228A156A197A218A159A192A195A160A218A161A2302方法与结果A113A58A57A162A163A165A166A165A166A75A76A98A242A190A89A191A90A91A93A94A96A47A2A97A7A99A98A178A173A175A174A90A90A200A201A100A174A90A90A187A175A203A90A181A251A252A167A74A242A218A117A254A174A100A155A90A89A96A168A46A169A4A171A172A173A174A175A177A178A73A178A155A71A242A72A201A181A251A252A179A242A247A180A181A183A184A186A183A193A187A188A234A189A192A194A188A195A196A197A187A183A188A199A198A202A196A205A206A188A207A209A210A211A212A202A213A215A216A217A219A220A222A224A225A227A229A196A205A181A181A23022A231A232A233A235A236A240A241A243A231A232A233A235A240A241A244A245A246A248A215A216A217A249A250A251A196A180A205A183A252A253A255A196A205A183A184A107A0A31A8A253A32A1A2A223A3A107A35A4A179A253A37A5A253A73A6A7A9A10A22A181A180A18A205MA25A206A11A12A206A13A14A15A28A16A29A17A13A7223A19A20A21A23A24A26A27A30A19A20A21A23A26A27A126A33A34A36A38A39A40A239A41A242A176A42A43A44A45A216A46A47A48A238A49A242A50A51A50A52A50A53A54A48A55A56A48A122A169A50A57A58A48A126A59A52A50A247A48A60A61A62A118A48A63A217A64A65A66A67A48A60A61A68A218A69A169A50A70A71A48A62A118A74A75A48A68A76A77A90A78A126A79A80A48A81A82A48A83A62A118A74A75A48A84A218A69A85A86A87A88A89A74A75A48A91A92A48A93A122A48A60A61A75A126A94A93A95A51A50A70A71A48A96A97A48A98A99A68A100A226A218A101A52A50A70A71A48A102A103A53A70A237A104A48A105A106A100A226A218A101A48A98A99A108A106A100A226A218A101A48A68A109A110A75A111A112A113A48A129A114A115A116A117A70A71A38A75A66A67A48A68A109A116A119A120A48A91A92A48A93A122A48A126A94A93A95A48A121A123A12424A125A127A128A130A131A132A125A127A130A131A133A46A134A135A136A137A46A138A139A140A141A89A142A143A144A145A146A147A148A155A149A34A48A133A150A79A151A53A152A153A154A34A112A95A89A46A134A156A157A158A159A48A46A123A153A154A34A112A95A48A160A161A170A162A95A172A163A164A165A48A166A167A139A75A168A118A48A171A173A174A175A177A178A172A180A89A181A182A183A184A48A185A172A180A186A187A188A189A48A148A155A149A34A138A97A190A168A118A12425A191A127A192A193A194A195A191A127A192A193A196A197A60A61A198A126A175A177A178A199A150A34A112A95A52A151A50A48A51A151A50A48A52A50A151A50A48A52A79A151A50A48A52A117A151A50A48A79A50A151A50LA200A201A202A203A204A205A207A208A209A210A200A211A212A213A214A215A219A200A220A221A222A224A225A227A228A229A230A231A232A233A200A214A215A219A219A234A235A227A236A229A230A240A232A233A200A241A243A233A244A245A246A248A249A250A251A2521A248A2531A254A255A29A0A1A2A1A2A3A4A5A6A7A8A9A10A11A12A13A14A15A16A17A18A19A20A21A22A23A24A25A26A27A28A30A31A32A33A26A34A33A25A30A35A26A36A36A36A36A35A26A33A35A35A35A37A25A26A35A35A35A35A38A39A40A41A42A43A44A45A42A43A126A46A47A103A48A49A50A51A52A171A53A150A75A51A52A171A54A249A55A56A57A130A58A104A54A105A104A130A74A59A60A203A197A54A117A71A49A50A51A52A171A61A62A63A64A65A66A67A68A144A237A237A69A106A58A59A58A54A70A72A73A181A76A77A107A60A69A78A59A108A89A74A51A52A171A79A61A62A63A64A65A66A67A68A237A60A69A80A81A82A78A54A70A72A73A181A76A77A107A60A69A58A59A108A83A84A179A109A146A85A38A39A86A87A88A255A44A45A126A103A48A150A75A51A52A171A90A239A91A242A176A92A93A94A95A96A97A98A110A238A99A242A60A81A60A80A60A237A100A54A101A60A54A78A54A82A54A80A81A54A59A78A102A111A112A130A74A59A60A203A197A54A117A71A150A75A51A52A171A113A63A64A65A66A67A68A79A181A76A77A107A60A69A58A114A108A54A115A116A59A108A54A118A119A150A75A51A52A171A101A59A78A102A120A121A122A123A109A146A85A38A39A124A125A127A255A44A45A125A127A126A103A48A238A99A90A239A91A242A176A92A93A94A95A96A97A98A110A238A99A242A60A81A60A80A60A237A100A115A128A129A131A132A133A150A75A51A58A134A54A135A50A59A69A237A136A150A75A51A52A171A79A97A137A138A139A140A141A54A111A112A97A137A142A150A75A51A52A171A54A143A117A122A249A55A79A63A64A65A145A147A54A148A149A118A119A242A63A64A65A145A147A79A181A76A77A107A60A69A81A60A108A90A151A107A58A100A110A115A116A59A108A54A152A153A123A109A146A85A38A39A154A155A156A157A44A45A156A157A83A84A158A126A159A160A63A64A65A145A147A79A150A75A51A90A115A128A129A131A132A133A54A239A91A242A176A92A93A94A95A96A97A98A54A238A99A242A60A81A60A80A60A237A100A161A58A162A54A163A114A134A54A111A112A164A165A63A64A65A49A50A51A52A171A80A69A58A247A197A54A135A50A46A47A59A69A237A136A150A75A51A52A171A97A137A138A139A166A167A103A79A168A169A170A187A54A130A74A54A117A122A63A64A65A145A147A143A70A72A172A173A174A85A148A149A175A118A59A85A177A38A178A180A182A183A184A155A156A157A44A45A185A186A180A182A184A155A156A157A188A189A190A191A192A193A194A18A195A19A20A21A11A194A196A18A195A198A199A11A194A196A18A195A200A201A11A194A196A18A195A5A202A204A194A205A195A206A207A208A33A25A26A36A34A30A33A35A26A33A34A33A209A35A26A33A34A35A35A35A26A24A35A209A27A33A35A33A26A35A35A209A25A26A36A36A33A28A35A26A33A34A209A209A35A26A33A34A35A35A35A26A24A35A35A36A36A36A26A33A24A24A34A26A209A35A28A27A35A26A33A34A30A30A35A26A33A34A35A35A35A26A24A35A36A35A33A35A35A26A33A24A25A34A26A33A35A28A209A35A26A33A34A34A27A35A26A33A34A35A35A35A26A24A35A30A209A33A35A33A26A28A27A34A34A26A35A35A35A30A35A26A33A34A209A34A35A26A33A34A35A35A35A26A24A35A33A33A36A36A26A35A27A33A26A35A33A205A28A34A26A35A33A36A33A35A26A33A34A24A33A35A26A33A34A35A35A35A26A24A35A34A34A33A35A33A26A28A35A27A34A26A35A35A27A33A35A26A33A34A209A27A35A26A33A34A35A35A35A26A24A35A25A28A33A35A33A26A209A27A30A25A26A36A30A30A24A35A26A33A34A209A33A35A26A33A34A35A35A35A26A24A35A33A27A36A36A26A27A24A36A25A26A36A27A36A36A35A26A33A34A33A36A35A26A33A34A35A35A35A26A24A35A33A30A36A36A26A36A24A210A211A212A213A213A214A215A216A38A39A217A218A184A219A220A88A219A220A135A50A46A47A145A147A117A122A138A139A54A49A56A57A221A238A222A103A238A99A74A51A79A145A147A130A223A117A122A54A148A149A175A118A237A85A177A224A225A226A227A228A229A230A231A178A180A182A232A233A234A220A88A185A186A227A228A229A230A180A182A233A234A235A235A236A240A38A236A241A192A243A244A245A192A243A246A10A11A194A196A18A16A18A195A248A244A10A11A250A251A194A196A18A16A18A195A252A253A254A255A64A0A1A2A35A28A35A33A35A24A35A26A35A24A35A34A35A26A35A24A35A34A35A26A35A24A35A34A252A253A254A255A64A0A1A2A35A28A35A24A35A30A35A26A35A24A209A27A35A26A35A24A209A27A34A35A26A35A24A209A30A252A253A254A255A64A0A1A2A35A28A35A24A33A33A35A26A35A24A35A27A35A26A35A24A35A27A210A211A213A214A213A215A212A215A60A69A60A237A60A1063讨论A37A3A144A115A128A129A131A132A133A79A119A98A15A48A54A120A4A54A185A22A5A37A3A79A123A6A7A8A142A111A63A64A65A141A144A9A97A115A128A129A131A132A133A10A147A79A125A229A123A142A111A15A48A54A126A73A11A8A171A167A168A169A139A117A122A73A145A147A54A63A7A127A118A123A85A12A13A128A104A138A139A14A11A8A171A167A168A169A16A117A115A128A129A131A132A133A113A37A3A79A63A64A65A79A122A147A111A17A138A139A85A129A138A139A130A18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