十二卷组培苗的鉴别（Identification of twelve lines of tissue culture plantlets）

1、十二卷组培苗的鉴别（Identification of twelve lines of tissue culture plantlets）Identification of tissue culture seedlings of twelve rollsI recently, too many friends asked me about this problem, we are asked in the end why plantlets not good? Is it true that such a shop is selling tissue culture seedlings? Th

2、ere are many about the propagation of tissue culture seedlings, so what should the real tissue culture seedlings be like? Where are the advantages and disadvantages of tissue culture plantlets? Here we briefly talk about tissue culture and tissue culture seedlings, especially to help you how to go t

3、o the district group seedlings.1, treat the state of mind of tissue culture seedling:As a lover, should look at culture technique with a calm and mentality, do not listen to the non professional advice, should check the Internet on tissue culture and Plantlets of information and data, it is more mea

4、ningful than hearsay.As a producer, should look at the tissue culture technology in a developing and learning attitude, tissue culture technology is who can not change, represent the general trend, the traditional mode of production and breeding will be replaced by the new mode of production and bre

5、eding, it is the change of productive forces.As a seller, you should see significant business opportunities for plant tissue culture with a keen eye, hold the top of the Pyramid minority high profits far from Pyramid ranked bottom of the popularization, significance and benefits of the.Finally, as a

6、 boutique collectors, the plant itself is not an antique antique, the older the more valuable, the new plant is more valuable, I think the plants look forward to the day when the collection of antique proliferation may need to rethink the idea. But the mass production of tissue culture is limited to

7、 boutique collectors impact, because collectors often keeps plants, seedlings are at most seedlings in the breeding, the time distance will never catch up, so the future will show a seedling plant cheap expensive situation, I think this is a normal trend.2. What is tissue culture plantlets?The techn

8、ique of tissue culture, refers to a part of the plants, the vegetative to obtain new (clone seedlings) process, also called plant cloning. We get the lateral bud, leaf, root, inserted beheaded seedlings, belong to the category of tissue culture. This concept may be misunderstood by many people.So wh

9、at is the organizational training that leads to market turmoil? Specifically, it should be rapid propagation in vitro, a branch of tissue culture - Rapid Propagation in vitro, referred to as micropropagation. This technology is inserted from the leaf, root cutting technology evolution, we use soil l

10、eaf cuttings propagation and root cutting, is made of synthetic matrix, the artificial matrix looks like jelly, but also by the change to the pot container glass bottle. This is the plant tissue culture factory that we all saw on tv.So why is jelly fast in glass bottles? The root causes are in the s

11、uperior environment and the role of plant hormones. The temperature, light and humidity in the laboratory are constant. Jelly contains enough nutrients and powerful plant hormones. These hormones can follow the growth state of human will adjust the plant, let it out or let it root sprouting, can con

12、trol (but it also depends on the operators experience and academic level, can calm operation hormones who is limited in China.).We usually do leaves when inserting and beheaded, some will use one hormone, including my shop is in the sale of these hormones, in fact, the nature and truth to the leaf a

13、nd plant hormone inserted beheaded dropping behavior and fast reproduction is the same, the seedling is completely a kind of. This is a lot of people have a great misunderstanding, and even think of different breeding methods get different seedling traits. In fact, the only difference is that nutrie

14、nt enrichment, is seedling plumpness, leaf cuttings is better than cut off some of the weak, showing the characters later, this is because the location of endogenous hormones and nutrition leads to, but not exactly the same genome, traits of drift. Only the chimeric traits, such as variegated, there

15、 may be a difference between the probability of learning in the leaf plug and beheaded, this problem will be detailed in the variegated topics.Back propagation , due to the dynamic changes of manipulation of plant hormones, the in vitro plant tissue can be in accordance with the wishes of the people

16、 of budding, a two a because asexual propagation of plants is considered to be infinite, so in the tissue culture of rapid propagation mode can be unlimited expansion of the this is the varieties of seed, harm the most deadly local market. However, it must be pointed out that infinite breeding seedl

17、ings also has a cost, not like Louis Lius magic fall from the sky, many people think that in no cost or low cost, and so on are not worth a hair of speech, do not understand the culture.We want to get tens of thousands of seedlings, there must be a special tissue culture laboratory or factory, this

18、operation cost is very large, it can be said that the general manufacturers can not do. So far, large tissue factories have been set up by the government. In other words, most people who produce tissue culture seedlings cost the state money. You cant afford to do your own tissue factory.3. Where are

19、 the advantages and disadvantages of tissue culture rapid propagation?Rapid propagation of seedlings, due almost entirely * hormone regulation is obtained, which leads to the question of whether people understand the hormone manipulation of plant hormones are familiar with the characteristics, theor

20、y and plant physiology, whether they have enough experience to manage tissue culture techniques, these points are decided by micropropagation seedling quality.If the strict micropropagation process, then the tissue culture and rapid propagation of the seedlings with leaf cuttings, beheaded seedlings

21、 are exactly the same, the greatest degree of retention of the complete genome of plants. This is the biggest advantage of micropropagation, which also determines the propagation of the most important crops, rare flowers and germplasm preservation are also * way of tissue culture and rapid propagati

22、on to do, even our life today, including dinner, with dense inseparable of the tissue culture and rapid. Transgenic plants also have to use tissue culture and propagation platform to complete, we live in contact with at least 30 kinds of genetically modified plants derivative products.Shortcoming:1)

23、 deficiency: hardening of tissue culture and rapid propagation of seedlings due to living in a perfect environment, the resistance and self-control is very poor. In other words, hormone * child, it needs to move from the laboratory to the domestication and Cultivation in greenhouse, line called hard

24、ening. After hardening of Tissueculture seedling quality and transplanting leaf beheaded, exactly the same, or even better. So where are the disadvantages? There are individual businesses in the tissue culture seedlings are not completely hardened under the circumstances of the sale, which led to th

25、e seedlings after the purchase of rapid liquefaction death.2) hormone residues: tissue culture and rapid propagation of seedlings due to accumulation of more hormones or hormone sensitivity, few seedlings in the late after the hair root showed some residual hormone effect, this effect is not more th

26、an one year. For example, relatively easy to sprout, bloom or more cycle disorder, abnormal root etc. But this situation is not unique to micropropagation seedling, hormone induced leaf cuttings and beheaded seedlings also can appear the symptom, even more exaggerated! Therefore, this is not the bas

27、is for identification of tissue culture plantlets. This usually happens when the seedlings adapt to the new environment and after 1 years, they will gradually improve until the transition is normal. Then again, only a handful of micropropagation seedlings will appear this kind of state, and not all,

28、 we get good seedling may also micropropagation seedling.3) influence on sexual reproduction because micropropagation obtained seedlings are needed after hardening seedlings, and long-term greenhouse cultivation can blossom, so in general the sexual reproduction has no effect. However, changes in ho

29、rmone sensitivity in very young seedlings can lead to later pollination problems, but most of them promote fruiting! This is interesting, that is to say, some tissue cultures are easy to pollinate and produce large amounts of seeds. One of the major rumours circulating is tissue culture, plant steri

30、lity, which is just the opposite! In fact, tissue culture plantlets are easier to obtain large amounts of seeds. I believe that many of the early cultivation of tissue culture in Shenyang rapid propagation of red lines, longevity, blue and white, Kang Pingshou friends should be deeply touched.4) roo

31、t primordium: due to abnormal hormone environment will lead to the original root Kiwi bundle arrangement disorder, which leads to the root may be inhibited, the normal root germination is not smooth. When general in the micropropagation seedling hardening, can cut off this part of the root primordiu

32、m, obtain normal roots, only a few do not understand the fleshy tissue culture will ignore this point operator. Experienced tissue culture operators will deliberately remove this part of the root primordium. If you do not remove this part of the root primordium case, it can be used as a sign of reco

33、gnition of seedling roots, is a very concentrated stem distorted, the leaves are narrow slender, with parallel deformity of the root or root primordium, if we get such a vaccine, it is actually very simple, with a knife can be completely cut off. But it must also be noted that the hormone induced le

34、af cuttings and seedlings may also be beheaded deformities of root primordium! The implication is that this is not the gold standard for tissue culture.4, how to identify tissue culture plantlets?:In the light of the problems mentioned above, it is clear that if a tissue culture plantlet is hardened

35、, and even after six to 1 years of greenhouse domestication, I can confidently say,No one can tell the difference. This is a tissue culture seedling.So how do we try to differentiate?1) first of all is to see the number and whether there are many similar seedlings, a new variety of assumptions, in t

36、he short term, obtained a large number of similar size approximate seedlings, then we can suspect that this is because of the large seedling, female * general cannot get beheaded and leaves into more than 10 strains above identical seedlings in general, there will be differences in size. But now the

37、 business is also very smart, may not be introduced so many times, and slowly put on the market. Its hard to tell. For example, two years ago saw sporadic ghost rock, I look back, there have been so many kind of ghost rock appeared, so we can suspect that this is the tissue culture, such as the Forb

38、idden City, green island and some varieties of these silver can be included in the suspect. But it must be objectively speaking, these plantlets and not what is not good, some people say that some characters with different standard plants, I think there were many reasons for this, and had to engage

39、in micropropagation so there is a close relationship between the choice of female quality, but also has a relationship with the late cultivation, can not be generalized. I bought a lot of tissue culture of the ghost rock city as a cross parent, I think it is very good, affordable and efficient! And

40、these ghost rock city after a period of time, the window will be bright (someone said, tissue rock ghost city window is frosted), so still think with cultivation environment closely related.2) look at the state of the seedlings: no hardening of the seedlings, usually tender green, water immersion, n

41、o root or abnormal roots, slender, like grass. These are the characteristics of Non Hardened tissue culture plantlets. If we get this kind of seedlings, in general, most fans can not afford to feed them, because the hardening of this step is very professional, and must be exogenous nutrients and hor

42、mones to maintain a smooth transition. Then after the hardening of the seedlings are roots, generally more sturdy, dark green or epidermal characteristic window appears, these seedlings are difficult to distinguish with beheaded seedlings, only a handful of people can engage in tissue culture, when

43、the hardening seedlings in culture after 1 years, the people to engage in tissue culture out: 3) the deformity of the seedling: if this kind of situation appears on the market, then I despise the person who does the tissue culture first. This kind of abnormal seedling usually should be thrown away i

44、n the process of production. Unless the miser crazy to get fraud on the market. Malformed seedlings are difficult to harden, and often this malformed seedling is a plant without definite growth points, or leaves twisted seriously into a package state, and new leaves are unable to sprout normally. If

45、 the leaf is only half wrapped or has many growth points and the growth point is obvious, this situation does not belong to the malformed seedling, but can only be regarded as the early separation state of the clump seedling. This kind of seedling can be hardened and cultivated normally. Plants spro

46、ut, due to the influence of endogenous hormones in leaves, will also appear half wrapped or distorted, and we use hormone treatment plant or leaf cuttings beheaded, can achieve similar tufted seedling, so long as not particularly deformity, and can not be identified as plantlets.4) root primordium d

47、eformity: tissue culture experienced rapid propagation is to solve this problem, only those inexperienced personnel will take this feature for people to identify: the findings have already mentioned, if we encounter this kind of situation, with a knife cut and root OK 5, which claims are mistaken fo

48、r the characteristics of tissue culture?1) transection of the base: no need to say that. Almost all asexual reproduction method will have the base of the cross section, some people in order to put this beautiful love into spherical surface. In short, this is clearly not a sign of tissue culture;2) n

49、o root: I think its more obvious. Whether or not root is related to many factors, especially the cultivation environment. Unless we take a strict control by another, if not the root, then can be suspected of tissue culture and rapid propagation (or unhardened). Hardened seedlings are easily hairy ro

50、ots. Even partially hardened seedlings root out 3 days after transplanting. Faster than normal seedlings.3) dry: only the hardened seedlings can be dried to distinguish the features, but the seedlings are generally not viable, and therefore can not be used as a sign. A lot of seedlings due to the re

51、latively small, the transportation will be dehydration, dehydration is to prepare for the new root germination, so this is also collapse of itself.4) not strong: just now. Tissue culture plantlets are generally easy to seed. Just the opposite.5) lost characters: this problem is not apparent when we

52、buy seedlings, it must grow up, many of the characters are age, nutrition and environment dependence. The question is so complicated that it can not be used as a sign. Objectively speaking, micropropagation is beheaded with leaf like plug,There should be no abnormalities in the way the genome is com

53、pletely preserved. The mutation caused by the hormone is only a very different case. The mutant is generally regarded as a new breed or rarity and will not be sold as a common seedling.6) deformity: only serious deformities, may be questioned is tissue culture seedlings, this is not a gold standard.

54、 In general asexual reproduction, at the beginning few are not deformed.7) harm to the growers health: this statement includes the fact that tissue culture seedlings can cause infertility and carcinogenicity. Its ridiculous. When you hear it, be a laughing stock.6, suppose we get tissue culture plan

55、tlet and suspect it is tissue culture seedling, what should we do?If the suspect is tissue culture, then only a little fine cultivation. Theres no better way. If it is not hardened seedlings, fine will die. This is only true of businessmen.Suppose we get the hardened seedlings, and the businessmen a

56、lso tell them that this is tissue culture, then we should first ask whether it has been hardened How long has it been grown after hardening? If the hardening has been cultivated for more than 1 years, then we can be planted as a general seedling, if less than a year, you can then turn the basin next

57、 year when you remove all the roots, and re rooting, you can.7, the business responsibility:Tissue culture seedling itself is not terrible, terrible is business cover up!Our domestic businesses not to comment, of course, people engage in domestic culture in the light of the limited, most of them in

58、the ground.I just want to say, look at the Holland based company culture and Western website attitude, is it plantlets marked out, its what the fans told to help you to plant better, but the key is a manifestation of integrity, can lead the normal healthy development of the market. Only we all under

59、stand the culture, to avoid more misunderstanding and confusion in the market, rather than blindly rejection or cry up wine and sell vinegar, this ought to be returned to the right to choose the majority of fans and consumers! As a member of tissue culture, we have the obligation to publicize the relevant