当归和阿魏酸钠抗动脉粥样硬化的作用及其机制课件

1、当归和阿魏酸钠抗动脉粥样硬化,的作用及其机制,欧阳静萍等,2005.6,前言,动脉粥样硬化,在今天的世界上，心血管疾病比其他任何疾病杀,死更多的人，并使千百万人致残。,当归,传统活血化瘀中药,阿魏酸钠,当归的主要活性成分,当归和阿魏酸钠抗动脉粥样硬化的作用,家兔,对照组,普通饲料喂养,10,周,动脉粥样硬化组,高脂饲料,80g/,只,（,1g,胆固醇,5g,炼制猪油）,动脉粥样硬化阿魏酸钠组,阿魏酸钠,0.2,g/,天,动脉粥样硬化当归组,25%,当归注射液,8ml /,天,减少主动脉粥样斑块面积,高脂组,阿魏酸钠组,当归组,作用,各组动脉粥样硬化斑块面积比较,N,*p0.01,vs,AS g

2、roup,Group,Plaque area,Control,6,0,AS,6,63.31,7.46,AS+SF,6,23.20,6.67*,AS+Ag,6,35.58,7.25*,作用,血脂变化,Group,N,Tch,TG,HDL-ch,LDL-ch,Control,6,0.95,?,0.21,0.87,?,0.18,0.46,?,0.14,0.52,?,0.15,AS,6,11.79,?,0.23*,3.52,?,0.63*,1.63,?,0.27*,8.23,?,0.30*,AS,SF,6,11.89,?,0.17*,1.75,?,0.53*,1.47,?,0.20*,8.51,?,0

3、.39*,AS,Ang,6,11.82,0.08,*,1.68,0.26,*,1.53,0.26,*,8.45,0.31,*,作用,血浆,t-PA,和,PAI,活性,组别,N,t,PA,PAI,control,组,6,0.67,?,0.08,0.72,?,0.11,AS,组,6,1.11,?,0.09,*,0.31,?,0.08,*,AS,SF,组,6,0.88,?,0.21,0.11,?,0.04,*,作用,血液流变学变化,2.43,0.66,control,组,AS,组,AS+SF,组,全血粘度,8.14,1.72,5.23,0.75,*,8.35,0.96,血浆粘度,1.31,0.18,

4、1.36,0.09,1.45,0.12,红细胞,压积,%,）,34.60,2.15,26.0,2.38,*,28.83,3.02,*,血沉方程,K,值,4.69,3.22,1.94,0.69,3.67,1.29,红细胞聚,集指数,1.95,0.26,2.69,0.21,纤维蛋白,原,2.62,1.57,1.31,0.23,2.79,1.4,AS+ANg,组,6.17,0.18,1.35,0.11,31.4,2.41,2.22,0.25,2.00,0.22,1.81,0.37,作用,主动脉超微结构观察,作用,血管内皮细胞形态变化,control HLS,HLS+SF,HLS+Ang,作用,con

5、trol,HLS,HLS+SF,HLS+Ang,作用,血管内皮细胞存活率,Groups,Survival rate of EC(%),Control group,92.3,*,35.4,OLDL,+SF group,86.8,OLDL group,OLDL+angelica group,88.7 *,作用,正常内皮细胞,ICAM-1,的表达,机制,剪切应力对内皮细胞,ICAM-1,表达的影响,机制,(2 Dyn/cm2,，,4h),当归对切应力损伤后内皮细胞,ICAM-1,表达的影响,机制,正常内皮细胞骨架结构,机制,TNF,对细胞骨架的作用,机制,当归对,TNF,损伤后细,胞骨架的保护作用,

6、机制,ICAM-1,免疫组化,正常对照组,OLDL,组,OLDL+,当归组,机制,细胞培养液中,NO,含量的变化,Group,N,NO content,（,nmol/ml,）,Control,6,0.346,0.06,HLS,6,HLS+ Ang,6,0.537,0.064*,HLS+SF,6,0.183,0.056*,0.367,0.039*,机制,血管内皮细胞,eNOS mRNA,和,iNOSmRNA,活性的表达,iNOSmRNA,eNOSmRNA,机制,内皮细胞中,TGF1,及,bFGF,表达,Control HLS HLS+SF,机制,bcl-2,正常组,bcl-2 TNF-,组,bc

7、l-2 TNF-,+,阿魏酸钠组,bax TNF-,+,阿魏酸钠组,bax TNF-,组,bax,正常组,血管内皮,bax,、,bcl-2,表达,Group,Cell,number,Average optical density,Bcl-2,Bax,Control,100,0.182,0.016,0.147,0.012,SF,100,0.181,0.014,0.147,0.019,TNF-,100,0.149,0.010*,0.190,0.015*,TNF-,+SF,100,0.179,0.011*,0.150,0.014*,机制,各组,NF-,B,变化,正常组,TNF,组,TNF+SF,组,

8、机制,内皮细胞核转录因子,（,NF-,B,）,表达,Control,TNF-,SF,TNF-,机制,当归对,Cu2+,介导,LDL,氧化,的浓度效应曲线,0,5,10,15,L,D,L,L,D,L,+,C,u,2,+,0,.,0,3,1,2,0,.,0,6,2,5,0,.,1,2,5,0,.,2,5,0,.,5,1,2,4,8,1,6,Angelica concentration (mg/ml),M,D,A,(,n,m,o,l,/,L,),机制,结,论,?,当归注射液和阿魏酸钠都可减少高脂饮食导致,的动脉粥样硬化斑块面积,?,降低高脂血症家兔血清脂质过氧化产物和,TG,的,含量；,?,增加红细胞，改善红细胞聚集性和变形性；,?,减少主动脉壁泡沫细胞形成，保护内皮细胞；,?,改善血液流变学；,作