医学免疫学资料：2003-7-B-da

1、The final examination of medical immunology for seven year students of Grade 2003（2005/12/6） Paper BExplain the following items （2 points for each,18 points）1. McAbPrepared by hybridomas technique (1 point) Immunized spleen cells(B) hybride with myeloma cells hybridoma (1 point)2. MACC5b6789 complex

2、 is known as MAC whichleads to formation of a hole in the membrane.(1 point) MAC mediates lysis of target cell.(1 point) 3. HaptenSubstances which can combine with Ab, but cannot induce immune response independently.(1 point) In another word, hapten only possess immunoreactivity.(1 point)4.TCR compl

3、exa group of membrane molecules on T cells that can specially bind to the antigen and pass an activation signal into the cells (1 point) consisting of TCR, CD3 and zz (1 point)5.Hypersensitivitya harmful immune response that is heightened reactivity to an antigen is called hypersensitivity, (1 point

4、) which causes tissue injury or functional disorder.(1 point) 6.Clonal anergyfunctional inactivation without cell death(1 point) : lack co-stimulatory signal(1 point).7.Heterophile Ag(forssman Ag):common Ags are shared by different species -no specificity of species (1 point)- Significance : immunop

5、athology ,Diagnosis (1 point)8.ITAMimmune receptor tyrosine-based activation motif (0.5 point)-transduce activation signals from TCR(1 point) composed of tyrosine residues separated by around 18 amino acid-when antigen binds,the tyrosine become phosphorylated by the receptor associated tyrosne kinas

6、 to transduct activtory signal(0.5 point)9.PRRThe receptor on macrophage (1 point)which can recognize and bind specific molecular structure on some pathogens,injured or apoptotic cells. (1 point)II. Choose all correct answers (1 point for each, 15 points)No.12345678AnswerACDCDACACDBCDABDBDBDNo.91011

7、12131415AnswerABDBCDABCABCABCDABCDABC。Answer the following questions briefly(5 point for each, 30points)1. There are two different receptors expressed on NK cells: KAR which can recognized glucide ligand distributing on tumor cells and virus-infected cells and transduct activtory signals by ITAM str

8、ucture and KIR which can recognize MHC-expressed on self-organic cells and transduct inhibitory signals by ITAM structure.2. 1). Ig superfamily(IgSF) Act as co-receptor and co-stimulatory signal during the recognition and activation of immune cells.2).Integrin familyParticipate in the adhesion of le

9、ukocyte and vascular endothelial cell during inflammation. 3) Participate in lymphocyte homing.Mature lymphocyte migrate to peripheral lymphoid organsRecirculation of lymphocytesLymphocyte migrate to the sites of inflammation 3. 1). Induction of immunologic tolerance to antigen in fetal period and n

10、eonate periodPrevent the rejection of organ allografts and xenograftsTreat autoimmune diseasesTreat allergic diseases 2). To terminate immunologic toleranceTo treat tumor: enhance first signal or second signalTo treat infection diseases 4. Type I； 1）Allergen-stimulated period Antigen binding to IgE

11、cross-links FcR molecules on the mast cells induces the release of mediators (degranulation)：2）Sensitization against allergens3）The activators of mast cellsMast cell activation products： histamine leukotrienes-Preformed mediators5. 1) Alternative pathway: Stabilization of C3 convertase - Certain bac

12、teria or their products (lipid polysaccharides, etc.), provide a protected (activator) surface for C3b and C3bBb.Morover, binding of another protein,factor P, further stabilizes this complex Generation of C5 convertase - Stabilized C3 convertase cleaves more C3 and produces C3bnBb complex , the C5 c

13、onvertase which cleaves C5 into C5a and C5b. 2 )MBL pathway : (1)binding stage Mannan-binding lectin (MBL) binds mannose of bacteria and interacts with serine protease resulting in MBL-associated serine protease (MASP). MASP is analogous to C1 and leads to cleaving of C4 and C2. (C3 convertase) (2)

14、Activation stage6. There are three highly diversity stretches within the V egion which called CDRAg-binding sitesIdiotype of Ig Answer following question (12 points)1. innate immune 1) Activation of complement system : Cell lysis, Opsonization ,ADCC, elimination of immune complex 2) Non-specific immune cells: macrophage cells(eosinophil ,M ect)，NK cells(ADCC) or by serecti