中国药科大学药学专业英语课件六味地黄丸

1、六六 味味 地地 黄黄 丸丸 Liuwei Dihuang Pills IngredientsnRadix Rehmanniae Preparata 160 g 熟地黄熟地黄nFructus Corni (processed) 80g 山茱萸（制）山茱萸（制） nCortex Moutan 60 g 牡丹皮牡丹皮nRhizoma Dioscoreae 80 g 山山 药药nPoria 60 g 茯茯 苓苓nRhizoma Alismatis 60 g 泽泽 泻泻 Procedure/制法制法 Pulverize the above six ingredients to fine powder,

2、 sift and mix well. To each 100 g of the powder add 3550 g of refined honey and a quantity of water to make pills and dry; alternatively add 80110 g of refined honey to make small honeyed pills or big honeyed pills.pulverize: 研磨研磨 sift：评审，过筛评审，过筛 to make pills : 泛丸泛丸 Description Brown-black water-ho

3、neyed pills, black-brown small honeyed pills or big honeyed pills; taste, sweet and sour. Identification Microscopical: n Starch granules triangular-ovoid or oblong, 2440m in diameter, hilum short cleft or V-shaped. nIrregular branched masses color less, dissolved in chloral hydrate solution: hyphae

4、 colorless, 46m in diameter. nParenchyma greyish-brown to black-brown, cells mostly shrunken, and each containing a brown nucleus-like mass. cleft：裂缝裂缝 hyphae：菌丝菌丝nClusters of calcium oxalate occurring in colourless parenchymatous cells, sometimes several clusters arranged in rows. Epidermal cells o

5、f pericarp orange-yellow, polygonal in surface view, with somewhat beaded anticlinal walls. Parenchymatous cells subrounded, with elliptical pits, gathered into pit groups.epidermal: 表皮的表皮的 pericarp: 果皮果皮 pit：纹孔纹孔beaded anticlinal walls: 垂周壁念珠状增厚垂周壁念珠状增厚(2) Physical and Chemical identificationn To 9

6、 g of small honeyed pills or big honeyed pills, or 6 g of water-honeyed pills, in powder, add 4 g of kieselguhr and triturate well. nHeat under reflux with 40 ml of ether at a low temperature for 1 hour, filter and evaporate to remove ether. kieselguhr: 硅藻土硅藻土 triturate: 研匀研匀 nDissolve the residue i

7、n 1 ml of acetone as the test solution. Dissolve paeonol CRS in acetone to produce a solution containing 1 mg per ml, as the reference solution. Carry out the method for thin layer chromatography (Appendix B), using silica gel G as the coating substance and cyclohexane-ethyl acetate(3:1) as the mobi

8、le phase. paeonol: 丹皮酚丹皮酚 cyclohexane: 环己烷环己烷nApply separately to the plate 10l of each of the two solutions. After developing and removal of the plate, dry it in air, spray with 5% solution of ferric chloride in ethanol acidified with hydrochloric acid and visualize under a current of hot air. The

9、bluish-brown spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the reference solution. AssaynDistil 2 g of the big honeyed pills (or 5 g of the water-honeyed pills or small honeyed pills), accurately weighed, col

10、lect 450 ml of distillate, transfer to a 500 ml volumetric flask and dilute with water to volume. Carry out the method for spectrophotometry (Appendix A), measure the absorbance at 274 nm and calculate the content of paeonol (C9H10O3), taking 862 as the value of E1%1cm. The big honeyed pills contain

11、 not less than 0.63%, the small honeyed pills contain not less than 0.07% and the water-honeyed pills contain not less than 0.10% of paeonol (C9H10O3), refered to Cortex Mountan.distil: 蒸馏蒸馏 volumetric flask: 容量瓶容量瓶 spectrophotometry: 分光光度法分光光度法Fructus Cornin To 5 g of pills, accurately weighed, add

12、 30 ml of water, allow to disintegrate thoroughly on a warm water bath, add 2 g of the kieseguhr, stir well and filter through filter paper. Wash the residue with 30 ml of water, dry at 100 and grind into fine powder. Transfer the powder together with the filter paper to a Soxhelts extractor and hea

13、t under reflux with an appropriate quantity of ether for 4 hours. disintegrate/溶解；溶解； grind/成粉末；成粉末； Soxhelts extractor/索氏提取器索氏提取器 Recover the ether and macerate the residue with two 15ml quantities of petroleum ether (3060) for about two minutes each time. Decant the petroleum ether, dissolve the r

14、esidue in a mixture of absolute ethanol-chloroform(3:2) by warming. Transfer accurately the solution to a 5 ml volumetric flask, dilute to volume, mix well, use the solution as the test solution. macerate/浸软；浸软； decant/倒出；倒出； absolute ethanol/无水乙醇无水乙醇 Dissolve ursolic acid CRS, weighed accurately, i

15、n absolute ethanol to produce a solution containing 0.5 mg per ml as the reference solution. Carry out the method for thin layer chromatography (Appendix B), using silica gel G as the coating substance and a mixture of cyclohexane-chloroform-ethyl acetate-formic acid(20:5:8:0.1) as the mobile phase.

16、 Apply separately to the plate 5 l test solution of the water-honeyed pills or 10 l of the test solution of the big or small honeyed pills, 4l and 8 l of the reference solution alternately. ursolic acid: 熊果酸熊果酸 formic acid: 甲酸甲酸 After developing and removal of the plate, dry it in air, and spray wit

17、h 10% solution of sulfuric acid in ethanol. Heat at 105 for 57 minutes to the spots clear. Cover the plate with a glass plate of the same size and fix up with adhesive plaster. Carry out the method for thin layer chromatography ( thin layer chromatographic scanning method. Appendix B), scan at wavel

18、ength: S=520 nm, R =700 nm. Measure the integration values of absorbance of the test solution and the reference solution, calculate the content of ursolic acid in the egration values: 吸光度值吸光度值 The big honeyed pills contain not less than 1.17mg per pill; the small honeyed pills and the water-honeyed pills contain not less than 0.13 mg and 0.20 mg pe