大气PM与心血管疾病PPT学习教案

1、会计学1大气大气PM与心血管疾病与心血管疾病第1页/共42页Scanning electron microscopy (SEM) observations. The particulate matter (PM) samples collected in the rural site, in the urban site or the industrial site have been studied using SEM. According to their respective sampling sites and, therefore, origin, some morphologic

2、diff erences between the PM from the three collected samples were observed.第2页/共42页第3页/共42页最受关注的是可吸入颗粒物(PM 10) 粗颗粒物 PM2.5-10 细颗粒物（PM 2.5) 超细颗粒物 (PM0.1) PM简介简介第4页/共42页第5页/共42页第6页/共42页第7页/共42页第8页/共42页第9页/共42页第10页/共42页第11页/共42页第12页/共42页第13页/共42页第14页/共42页第15页/共42页第16页/共42页第17页/共42页第18页/共42页Fig. 1. Summa

3、ry of the pathways by which particulate matter promotes acute cardiovascular events and atherosclerosis. Mechanisms can be broadly divided into direct and indirect pathways.第19页/共42页第20页/共42页第21页/共42页第22页/共42页第23页/共42页第24页/共42页第25页/共42页大气PM在体研究动脉硬化？心功能？心律失常？凝血功能？机制研究（细胞培养、分子生物学、膜片钳技术等）提出防治措施第26页/共42

4、页第27页/共42页注：与对照组相比a：P；与10g/ml组相比b： P；与100g/ml组比较c：P；与SOD+DMSO+PM100组比较d：P 前期实验结果（一）内皮完整各组间收缩率比较第28页/共42页 注：各组间比较 前期实验结果（二）去内皮各组间收缩率比较第29页/共42页注：与对照组相比a：P、A ：P；与10g/ml组相比b： P、B ：P；与100g/ml组相比c：P 前期实验结果（三） 内皮完整各组间舒张率比较第30页/共42页第31页/共42页入细胞核Nrf2入细胞核ARE介导的二相酶基因如HO-1等表达蛋白、脂质、核酸过氧化、线粒体受损大气PM2.5致内皮细胞氧化应激可能

5、机制（参考相关文献完成）PMROSMAPK PI3K/AktPKCKeap1/Nrf2科学设想第32页/共42页Nrf2Keap-1胞胞 浆浆细胞核核膜细胞核核膜ARE二相酶基因表达增多二相酶二相酶第33页/共42页实验结果（一）第34页/共42页实验结果（二）第35页/共42页Western blot analysis shows that protein expression of HO-1and Nrf2 was induced with PM2.5 treatment in HUVEC cells. B. densitometric analyses of HO-1 and Nrf2、

6、Keap1 protein amount of Nrf2, Keap1, or HO-1 protein was normalized to GAPDH protein， Results are expressed as -fold induction compared with that of control. Data expressed are . (n=3). *, P0.05; *,P 0.01 versus control HUVEC cells，#, PPM2.5 concentration(g/ml)contral50100 protein expression of HO-1/GAPDH(%)0.0.51.01.52.02.5 *#PM2.5 concentration(g/ml)contral50100protein expression of Keap1/GADPH(%)0.0.2.4.6.81.01.2PM2.5 concentration(g/ml)contral50100protein ex