脑组织中的海马取法

1、step 1:This is a dorsal view of a mouses skull, in order to access the brain you can cut along the coronal suture and sagittal suture then pull off both sides of parietal bone and interparietal bone step 2: Now the skull is open and you can see the brain clearly, in order to get the hippocampus youd

2、 better keep the brain in the cranial cavity so the brain will notmove when you continue with the following steps step 3: In order to expose the hippocampus you need to remove the cerebral cortexcovering it. The first incision is at the end of the hemisphere; the incision should be about 0.7mm deep

3、for most adult mouse that you might not hurt the hippocampus while to expose it. The 2nd incision is about 1.5-2mm in front of the first one, this incision you need cut into the lateral ventricle, both of the incisions go to the ventralof the brain and meet there. Now this piece of cortex is free, p

4、ull it up, you will see the hippocampus just like in this picture, also you can see the CSF in the opened ventriclestep 4: Keep working on the other side of the brain pull up both sides of the cortexthat covering the hippocampus along the ventricle. Now you can see the dorsal part of the hippocampus

5、. Separate the rest of the hippocampus from the cortex covering it along the surface of the hippocampus towards the ventral part of the hippocampusstep 5: Keep working on the other side of the brain pull up both sides of the cortexthat covering the hippocampus along the ventricle. Now you can see the dorsal part of the hippocampus. Separate the rest of the hippocampus from the cortex covering it along the surface of the hippocampus towards the ventral part of the hippocampus. step 6: Now you need to free the hippocam