教育部暑期尖端人才培育计画 中兴大学干细胞与组织工程学计画

1、教育部暑期尖端人才培育計畫 中興大學幹細胞與組織工程學計畫課程：幹細胞學 授課老師 中興生科系 蘇鴻麟學員姓名 學號 1. Transgenic mice are often generated to characterize the ability of a promoter by fusing them to reporter genes. Which of the following are not commonly used transgenic reporter genes (1) LacZ (2) GFP (3)CAT (4)Luciferase2. To generate tra

2、nsgenic mice, microinjection of DNA directly into the fertilized eggs is the most popular approach. The DNA should be injected into: (1) female pronucleus (2) male pronucleus (3) mitochondria (4) fused zygotic nucleus. 3. To generate a gene manipulated mouse, the least time you will need from have t

3、he targeting construct to obtain a homologous mutant mouse is (1)2 weeks (2) 10 weeks (3)15 weeks (4) 22 weeks 4. Somatic “stem” cells from one tissue may give rise to cell types of another tissue. This is a phenomenon called 1). stem cell property 2). stem cell plasticity 3). stem cell niche 4). tr

4、ansdifferentiation5. Somatic cells are converted from one tissue type to another tissue type called 1). stem cell property 2). stem cell plasticity 3). stem cell niche 4). transdifferentiation6. Recent published works suggested human dermal fibroblast can be reprogrammed to “pluripotent” stem cells

5、by define factors-Oct4, KLF4, Sox2 and c-Myc. This is a phenomenon called 1). pluripotent reprogramming 2). cell lineage reprogramming 3). transdifferentiation 4). metaplasia.7. 應用於分離cancer stem cell的方法主要包括四種，請問何種分離方法中cancer stem cell mitogen的添加扮演重要角色？1). Clonogenic assay; 2). Surface antigen-based

6、purification; 3). Side population-based purification; 4). Label-retaining-based purification。8. 請問cancer stem cell的來源為何？。1). Stem cell; 2). Progenitor cell; 3). Differentiated cell; 4). 以上皆可。9. 組織工程應用中需要cell、scaffold、biomolecule等。其中最常被應用的細胞型式為何？ 1). Embryonic stem cell; 2). Adult stem cell; 3). Matu

7、red cell; 4). Cancer stem cell。10. cancer stem cell的腫瘤產生特性需透過xenograft模式，目前研究表示能成功誘發腫瘤產生的最少量cancer stem cell為何？ 1). 10 cells; 2). 100 cells; 3). 1000 cells; 4). 10000 cells。11. Cancer stem cell可從病人組織分離或從細胞株分離，請問目前研究資料顯示兩者cancer stem cell的特性為何？1). 相近; 2). 不同; 3). 相反; 4). 不可比較。12. Nestin、CD133等為gliobl

8、astoma multiforme衍生cancer stem cell的分離追蹤markers，請問cancer stem cell分化過程中，nestin、CD133的變化為何？1). 表現增加; 2). 表現降低; 3). 表現不變。13. To generate induced pluripotent stem cells with Oct4, Sox2, Myc, and Klf4 virus, which of the following cell types is not suitable to use (1) skin cells (2) fibroblasts (3) matu

9、re B cells (4) none of above. 14. Mouse embryonic stem cells derived from inner cell mass cannot differentiate into (1) blood cells (2) placenta cells (3) muscle cells (4) neuron cells. 15. The difference between adult stem cells and embryonic stem cells is (1) pluripotency (2) immortalization (3) o

10、ncogenicity (4) all of above 16. spemann organizer 的發現是因為此組織可誘導1). 表皮 2). 神經 3). neural crest 4). 中胚層17. 此誘導作用，spemann organizer是因為分泌何種物質：1). Wnt 2).sonic hedgehog 3).BMPs 4).anti-BMP factors18. 脊索可誘導腹側神經管細胞，如motor neurons的形成，是因為分泌：1). Indian hedgehog 2). sonic hedgehog 3). Wnt 4). anti-BMP factors1

11、9. 哺乳類動物分裂到八顆細胞時，會進行compaction，是因為細胞表現1) P-cadherin 2) E-cadherin 3) N-cadherin 4) protocadherin20. 決定totipotent and pluripotent的時期在於1) 1 to 2- cells 2) 2 to 4-cells 3) 4 to 8-cells 4) 8 to 16-cells21. teratocarcinoma的由來細胞為胚胎的1) 生殖母細胞2) 皮膚幹細胞3) 毛髮幹細胞 4) 血液幹細胞22. 對老鼠ES細胞而言，何者定義最重要 1) 形成teratoma or te

12、ratocarcinoma 2)形成chimera mouse 3) germline transmission 4)可於體外繼代是非題23. To maintain the pluripotency of iPSCs, we need to continuously overexpressed Oct4, Sox2, and KLF4 gene with virus or plasmid. no24. It is impossible to generate parthenogenesis mice by any method. no25. iPSCs can generate a live

13、 mouse when it was injected into a 4N blastomere. yes26. To avoid misinterpretation of results affected by insertion locus, usually 3 independent lines of transgenic mice are required. The expression levels of transgene in these three lines are identical because they have identical promoter. no27. I

14、f the iPS cells were generated from mature B cells, the mouse generated from such ES cell will have only one type of antibody. yes28. Parabiosis provides a powerful approach to determine whether stem cells migrate through vascular system and contribute to long distance tissue regeneration and repair. yes29. 哺乳類受精卵分裂過程中，為1 2 4 8顆細胞，無單數細胞的產生。no30. 哺乳類受精卵發育過程中，早期細胞的分裂到八顆細胞時，細胞並不需要做出新的mRNA與蛋白質。no31. 培養human ES cell