HAPSITE顶空操作培训

1、Copyright 2009HAPSITE ER/Smart Plus Training HAPSITE ER/Smart Plus 培培 训训Headspace Sampling System 顶空采样系统Copyright 20092HSS Module Outline HSS 模件概要模件概要 HSS Assembly HSS 装配装配 HSS Theory HSS 原理原理 HSS Internal Standards HSS 内部标准内部标准 Preparing Blanks/Samples 准备空样准备空样/样品样品 Running Analyze 运行分析运行分析 Quantit

2、ation Reports 定量报告定量报告 Contamination Issues 污染问题污染问题Copyright 20093What is the Headspace Sampling System 什么是顶空采样系统什么是顶空采样系统 HAPSITE accessory HAPSITE 辅件辅件 Sampling system for: 采样系统用于采样系统用于: Water 水水 Soil 土壤土壤 Liquids 液体液体 Detects VOCs 检测检测 VOCs Qualitative methods 定性方法定性方法 Quantitative methods定量方法定量

3、方法 PPB to PPT range PPB 至至 PPT 量程量程Copyright 20094HSS Assembly HSS 装配装配 Insert a charged battery into the HAPSITE 将充足电的电池插入将充足电的电池插入 HAPSITE Disconnect the HAPSITE power supply cord 断开断开 HAPSITE 的电源电缆的电源电缆连接连接Copyright 20095Connecting the Y-Cable for Stationary Use 为台式用法连接为台式用法连接 Y - 电缆电缆 As the nam

4、e implies, the cable forms a Y 正如其名正如其名, 电缆呈电缆呈 Y 形形Joined end 连接端连接端Split ends 分叉端分叉端Copyright 20096Connecting the Y-Cable for Stationary Use为台式用法连接为台式用法连接 Y - 电缆电缆 Connect the joined end to the HAPSITE power supply cord 将连接端连接至将连接端连接至 HAPSITE 的电源电缆的电源电缆NOTE: Align the dots on the cable with the do

5、ts on the cord 注注: 对准电缆上的点与电源线上的点对准电缆上的点与电源线上的点Copyright 20097Connecting the Y-Cable for Stationary Use为台式用法连接为台式用法连接 Y - 电缆电缆 Connect one split end of the cord to the HAPSITE 连接电缆的一个分叉端至连接电缆的一个分叉端至 HAPSITE Connect the other split end to the Headspace Sampling System 连接另一个分叉端至顶空采样系统连接另一个分叉端至顶空采样系统NO

6、TE: Align the dots on the cable with the dots on the instruments to properly connect 注注: 将电缆上的点与仪器上的点对准将电缆上的点与仪器上的点对准, 直至妥善地连接上直至妥善地连接上Copyright 20098Transfer Line 传输线传输线 Provides communication between the HAPSITE ER/Smart Plus and the HSS 提供提供 HAPSITE ER/Smart Plus 与与 HSS 之间的通讯之间的通讯 Sample travels

7、through the transfer line to the HAPSITE ER/Smart Plus 样品经过传输线传送给样品经过传输线传送给HAPSITE ER/Smart Plus HAPSITE ER Transfer Line 传输线传输线HAPSITE Smart Plus Transfer Line 传输线传输线Copyright 20099Transfer Line 传输线传输线 Encompassed by a foam sleeve 外包泡沫套管外包泡沫套管 Provides insulation 提供绝缘提供绝缘 Reduces power requirements

8、降低功率要求降低功率要求 Directional 方向性方向性 Multi-connector end connects to the HAPSITE ER Universal Interface 多接头端连接至多接头端连接至 HAPSITE ER 的通用接口的通用接口 For the HAPSITE Smart Plus, the direction of the transfer line is labeled directly on it HAPSITE Smart Plus 的传输线的传输线方向直接标注在它的上面方向直接标注在它的上面Copyright 200910Inserting

9、the Transfer Line 插入传输线插入传输线 For the HAPSITE ER, plug the end with the single LEMO connection into the HSS 对于对于 HAPSITE ER, 将带有单将带有单个个LEMO 接头插入接头插入 HSS Align the red dots 对准红点对准红点 For the HAPSITE Smart Plus, plug the side labeled This End to Accessory into the HSS 对于对于 HAPSITE Smart Plus, 将边上标志将边上标志

10、此端至附件端此端至附件端的的一端插入一端插入 HSS Align the red dots 对准红点对准红点Copyright 200911Inserting the Transfer Line 插入传输线插入传输线 Remove the probe from the HAPSITE ER/Smart Plus 从从 HAPSITE ER/Smart Plus 上卸下上卸下探头探头 To attach the transfer line to the HAPSITE ER 连接传输线至连接传输线至 HAPSITE ER Screw the Universal Bulkhead connecti

11、on into place 拧上拧上通用的防水通用的防水连接件连接件 Align the red dots 对准红点对准红点 Insert the LEMO connection 插插上上LEMO 接头接头 To attach the transfer line to the HAPSITE Smart Plus 连接传输线至连接传输线至 HAPSITE Smart Plus Align the red dots 对准红点对准红点 Insert the LEMO connection 插插上上 LEMO 接头接头 HAPSITE ER View HAPSITE ER 视图 Copyright

12、200912Portable HSS Use 便携式便携式 HSS 的用法的用法 The HSS is designed for portable use HSS 设计于便携式应用设计于便携式应用Requires a battery 需用电池需用电池 Open the front door of the HSS 打开打开 HSS 的前门的前门 Insert the battery into the slot 将电池插入槽内将电池插入槽内 The arrow on the battery will be facing the battery release button电池上的箭头指向电池释放按

13、钮电池上的箭头指向电池释放按钮 The INFICON label will be in the lower left-hand corner INFICON 的标签位于左下角的标签位于左下角Copyright 200913Portable HSS Use 便携式便携式 HSS 的用法的用法 Push battery into slot until a “click” is heard 将电池推入槽中将电池推入槽中, 直到听见直到听见 “咔哒声咔哒声” Verify that the battery is securely fastened 证实电池已被牢固地固定住证实电池已被牢固地固定住Co

14、pyright 200914Nitrogen Canister 氮气瓶氮气瓶 Insert the nitrogen canister插入氮气瓶插入氮气瓶Copyright 200915Turning on the HSS 将将 HSS 的电源的电源 on Press down on the POWER switch 按下按下电源电源 开关开关 The POWER switch is a toggle switch 电源开关电源开关是扳钮开关是扳钮开关 Once pressed, it will return to its original position 一旦按下一旦按下, 它将回到原位它

15、将回到原位 The word POWER will illuminate 文字指示灯文字指示灯 POWER 亮亮 If the Headspace is powered by the HAPSITE power supply, the RMT POWER (Remote Power) will also illuminate 如顶空使用如顶空使用 HAPSITE 的电源的电源, RMT POWER (遥控电源遥控电源) 也亮也亮 Close the front door 关上前门关上前门Copyright 200916Preparing Analyze 准备分析准备分析 The HAPSITE

16、 ER/Smart Plus will automatically begin preparing a Headspace method HAPSITE ER/Smart Plus 将自动将自动开始准备顶空方法开始准备顶空方法 All components will be heated to their set point temperature 全部组件将加热至它们的设定温度全部组件将加热至它们的设定温度 AutoTune will be run 自动调谐将运行自动调谐将运行 Concentrator Cleanout will be run if a concentrator is ins

17、talled 如安装浓缩器将运行浓缩器清理如安装浓缩器将运行浓缩器清理Copyright 200917HSS Theory HSS 原理原理 VOCs collect as a gas in the headspace above the liquid or solid sample 在液体或固体样品上在液体或固体样品上, VOCs作作为气体收集在顶空中为气体收集在顶空中 Samples need to heat for 15-20 minutes to reach equilibrium 需将样品加热需将样品加热15-20 分钟分钟, 达到达到平衡平衡 Equilibrium occurs

18、when the rate of VOCs leaving the sample is equal to the rate of VOCs returning to the sample 平衡发生于当平衡发生于当 VOCs 离开样品离开样品与返回样品的速率相等时与返回样品的速率相等时 Vapor Phase蒸气相蒸气相Liquid or Solid Phase 液相或固体液相或固体Copyright 200918Determining Equilibration Times 确定平衡时间确定平衡时间AREA 面积面积Copyright 200919HSS Sampling HSS 采样采样 T

19、he Headspace needle is concentric and has two openings 顶空针头是同心的顶空针头是同心的, 有两个开口有两个开口 Sample is drawn in from the top opening 样品样品从顶开口引入从顶开口引入 Nitrogen is bubbled out of the bottom opening 氮氮从底开口涌出从底开口涌出Copyright 200920Sensitivity Tree 灵敏度树状图灵敏度树状图High Concentration高浓度高浓度LowConcentration低浓度低浓度LowSensi

20、tivity低灵敏度低灵敏度HighSensitivity高灵敏度高灵敏度ER_HSS_PPT_StandardHSS_Tri-Bed_PPT_Standard (low 低低 ppt)ER_HSS_PPB_StandardHSS_Loop_PPB_Standard(low 低低 ppb high高高 ppt)ER_Air_Tri-Bed_PPB_StandardAir_Tri-Bed_PPB_Standard(low 低低 ppb high 高高 ppt)Survey 速查速查HAPSITE ER: 1 ppmHAPSITE Smart Plus: 10 ppmER_Air_Tri-Bed_

21、PPM_StandardAir_Tri-Bed_PPM_Standard(low 低低 ppm high 高高 ppb)Copyright 200921HSS Internal Standards HSS 内部标准内部标准 The matrix of the internal standard must match the sample matrix 内部标准的基质必须与样品的基质匹配内部标准的基质必须与样品的基质匹配 Liquid samples use a liquid internal standard 液体样品使用液体的内部标准液体样品使用液体的内部标准 Gas samples use

22、 a gas internal standard 气体样品使用气体的内部标准气体样品使用气体的内部标准 TRIS and BPFB are gas standards TRIS 和和 BPFB 是气体标准是气体标准 These standards will not be automatically injected into the HAPSITE during a HSS run 在在 HSS 运行过程中运行过程中, 这些标准不自动注入这些标准不自动注入 HAPSITE 中中 HSS internal standards are liquid HSS 内部标准是液体内部标准是液体 Stand

23、ards must be manually injected into each sample vial using a syringe 必须用针管手动将标准注入至每个样瓶中必须用针管手动将标准注入至每个样瓶中Copyright 200922HSS Internal Standards HSS 内部标准内部标准 Internal standards are used as: 内部标准用作为内部标准用作为: A reference for quantitative methods 定量方法的参考定量方法的参考 An aid to estimate concentrations 帮助估算浓度帮助估

24、算浓度 4 component mix 4 成分的混合物成分的混合物 (p/n 071-748) Fluorobenzene 氟苯氟苯 (250 g/mL微克微克/毫升毫升) Chlorobenzene-d5 氯苯氯苯-d5 (250 g/mL微克微克/毫升毫升) 1,4-dichlorobenzene-d4 1,4-二氯苯二氯苯-d4 (250 g/mL微克微克/毫升毫升) Bromopentafluorobenzene 氟溴苯氟溴苯 (BPFB) (500 g/mL微克微克/毫升毫升)Copyright 200923HSS Internal Standard Addition Concen

25、tration Calculation HSS 内部标准添加物浓度计算内部标准添加物浓度计算 V1C1 = V2C2 Initial Volume x Initial Conc. = Final Volume x Final Conc. 0.010 mL x 250 m mg/mL (IS) = 20 mL x ? Final Conc. m mg/mL Final Conc. = 0.125 m mg/mL = 0.125 ppm = 125 ppbKeep the same units on both sides of the equation 公式的两边保持相同的单位公式的两边保持相同的

26、单位Internal standard concentration will vary based on sample size内部标准浓度基于样品大小而变更内部标准浓度基于样品大小而变更Copyright 20092424HSS 内部标准添加物浓度计算内部标准添加物浓度计算 V1C1 = V2C2 初始容积初始容积 x 初始浓度初始浓度 = 最终容积最终容积 x 最终浓度最终浓度 0.010 毫升毫升 x 250 微克微克/毫升毫升 (IS) = 20 毫升毫升 x ? 最终最终浓度浓度 微克微克/毫升毫升 最终浓度最终浓度 = 0.125 微克微克/毫升毫升 = 0.125 ppm = 1

27、25 ppb 公式的两边保持相同的单位公式的两边保持相同的单位 内部标准浓度基于样品大小而变更内部标准浓度基于样品大小而变更Copyright 200925HSS Internal Standards HSS 内部标准内部标准Final concentrations of various IS additions 各种各种 IS 添加物的最终浓度添加物的最终浓度SampleConfigurationAddition10 mL Water20 mL Water5 g Soil10 g SoilLoop Method (HAPSITE Smart Plus only)PPB method (HAP

28、SITE ER and Smart Plus)10 L of 250 g/mL IS0.250 g/mL(250 ppb)0.125 g/mL(125 ppb)0.500 g/g(500 ppb)0.250 g/g(250 ppb)10 L of 500 g/mL IS (BPFB)0.500 g/mL(500 ppb)0.250 g/mL(250 ppb)1.000 g/g(1 ppm)0.500 g/g(500 ppb)PPT method (HAPSITE ER and Smart Plus)1.0 L of 250 g/mL IS0.0250 g/mL(25 ppb)0.0125 g/

29、mL(12.5 ppb)0.0500 g/g(50 ppb)0.0250 g/g(25 ppb)1.0 L of 500 g/mL IS (BPFB)0.0500 g/mL(50 ppb)0.0250 g/mL(25 ppb)0.1000 g/g(100 ppb)0.0500 g/g(50 ppb)Copyright 200926HSS 内部标准内部标准各种各种 IS 添加物的最终浓度添加物的最终浓度样品配置样品配置添加物添加物10 毫升水毫升水20 毫升水毫升水5 克土壤克土壤10克土壤克土壤环方法环方法 (仅仅HAPSITE Smart Plus)PPB 方法方法 (HAPSITE ER

30、和和 Smart Plus)10 微升的微升的 250 微克微克/毫升毫升 IS0.250 微克微克/毫毫升升 (250 ppb)0.125微克微克/毫升毫升 (125 ppb)0.500 微克微克/克克(500 ppb)0.250微克微克/克克(250 ppb)10 微升的微升的 500微克微克/毫升毫升 IS (BPFB)0.500 微克微克/毫毫升升 (500 ppb)0.250微克微克/毫升毫升 (250 ppb)1.000微克微克/克克(1 ppm)0.500微克微克/克克(500 ppb)PPT 方法方法 (HAPSITE ER 和和 Smart Plus)1.0 微升的微升的 2

31、50微克微克/毫升毫升 IS0.0250微克微克/毫升毫升 (25 ppb)0.0125微克微克/毫升毫升 (12.5 ppb)0.0500微克微克/克克(50 ppb)0.0250微克微克/克克(25 ppb)1.0 微升的微升的 500微克微克/毫升毫升 IS (BPFB)0.0500微克微克/毫升毫升 (50 ppb)0.0250微克微克/毫升毫升 (25 ppb)0.1000微克微克/克克(100 ppb)0.0500微克微克/克克(50 ppb)Copyright 200927Preparing Blank/Samples 准备空样准备空样/样品样品 In general, HSS

32、samples are prepared as follows: 一般一般, HSS 样品准备如下样品准备如下: Liquids 液体液体 Fill vials full 注入样瓶注入样瓶 满满 20 mL 20 毫升毫升 Solids 固体固体 Use 10 grams 用用 10 克克 Do not block needle opening 切勿阻塞针头的开口切勿阻塞针头的开口 Inject 10 L internal standard for PPB (or Loop) methods 为为 PPB (或环或环)方法注入方法注入10微升内部标准微升内部标准 Inject 1 L inte

33、rnal standard for PPT methods 为为 PPT 方法注入方法注入 1 微升内部标准微升内部标准Copyright 200928Preparing Blank/Samples 准备空样准备空样/样品样品 Prepare a blank using VOC-free water and internal standard 用无用无 VOC 的水和内部标准的水和内部标准 准备空样准备空样 Verifies that the Headspace and the HAPSITE ER/Smart Plus are operating properly 证实顶空和证实顶空和 HA

34、PSITE ER /Smart Plus 的运行正常的运行正常 Proper gas flow 气流量正确气流量正确 Acceptable pressure 压强可接受压强可接受 Monitors carryover or contamination 监测残留或污染监测残留或污染Copyright 200929Preparing the Blank/Samples 准备空样准备空样/样品样品 Add 20 mL of VOC-free water into the vial 在样瓶中添加在样瓶中添加 20 毫升无毫升无 VOC 的水的水 Clean the 10 L glass syringe

35、 three times with methanol 用甲醇清洗用甲醇清洗 10 微升玻璃针管三次微升玻璃针管三次 Allow syringe to completely fill with methanol 针管可完全充满甲醇针管可完全充满甲醇 Discard excess methanol into waste vial 将过量的甲醇倾倒入废瓶中将过量的甲醇倾倒入废瓶中Copyright 200930 Practice proper syringe technique using methanol 用甲醇实习正确的针管技术用甲醇实习正确的针管技术 Draw the syringe plun

36、ger back to the 1 l mark 将针管柱塞拉回至将针管柱塞拉回至 1 微升标记处微升标记处 Insert the tip of the syringe needle into the methanol 将针头的针尖插入甲醇中将针头的针尖插入甲醇中 Be careful not to move the plunger 注意不要移动柱塞注意不要移动柱塞 Carefully draw the plunger back to the 2 l mark 小心地将柱塞拉回至小心地将柱塞拉回至 2 微升标记处微升标记处 Withdraw the syringe from the vial

37、从样瓶中撤回针管从样瓶中撤回针管 Be careful not to move the plunger 注意不要移动柱塞注意不要移动柱塞Preparing the Blank/Samples-Syringe Techniques准备空样准备空样/样品样品-针管技术针管技术Copyright 200931Preparing the Blank/Samples-Syringe Techniques准备空样准备空样/样品样品-针管技术针管技术Draw the plunger back until there is a 1 L plug of liquid between the 1 L and 2

38、L mark 抽回柱塞直到有抽回柱塞直到有 1 微升液体在微升液体在 1 微升与微升与 2 微升标记之间微升标记之间Plunger will be drawn back to 4 mL 柱塞被抽回至柱塞被抽回至4 毫升毫升 Inject this liquid into a waste vial 将此液体注入废瓶中将此液体注入废瓶中Disregard small air pockets that may appear near the plunger 不管柱塞附近是否出现小空气泡不管柱塞附近是否出现小空气泡Repeat this same procedure for sample prepar

39、ation 为样品准备重复此同样顺序为样品准备重复此同样顺序Copyright 200932Inserting Blank and Purge Vial into the HSS 将空样和冲净样瓶插入将空样和冲净样瓶插入 HSS 中中 Open the top lid on the Headspace 打开顶空的顶盖打开顶空的顶盖 Lift the needle assembly 提升针头组件提升针头组件 Place the standard blank into a well 将标准空样置入槽中将标准空样置入槽中 Place an empty vial into another well 将

40、空的样瓶放入另一个槽中将空的样瓶放入另一个槽中 This is the Purge Vial 这是冲净瓶这是冲净瓶Copyright 200933Inserting Blank and Purge Vial into the HSS 将空样和冲净样瓶插入将空样和冲净样瓶插入 HSS 中中 Center the needle over the septum and gently insert it into the vial for the standard blank 为标准空样对中隔膜上的针头为标准空样对中隔膜上的针头, 将它插入样瓶将它插入样瓶 Needle guides can be u

41、sed to help position the needle针头导向有助于针头定位针头导向有助于针头定位 Close the top lid 盖上顶盖盖上顶盖 Allow the blank to heat for 15-20 minutes 让空样加热让空样加热 15-20 分钟分钟Copyright 200934Running Analyze 运行分析运行分析Touch RUN ANALYZE 按按运行分析运行分析 Nitrogen from the headspace needle and bubble into the sample 来自顶空针头的氮来自顶空针头的氮, 泡状进入泡状进

42、入样品样品 This will purge the sample to release VOCs dissolved in the water 这将冲净样品这将冲净样品, 释放溶解在水释放溶解在水中的中的 VOCsCopyright 200935Collecting a Sample 收集样品收集样品 Collecting Sample For screen收集样品用于收集样品用于窗口窗口 The Headspace will automatically collect a sample顶空将自动收集样品顶空将自动收集样品 No action is required from the user

43、 用户无需动作用户无需动作 Sampling is Done screen 采样完成采样完成窗口窗口 Sample collection is complete样品收集完成样品收集完成 Again, no action is required from the user 同样同样, 用户无需动作用户无需动作Copyright 200936GRAPH View 图形查看图形查看 Compounds will be displayed on the front panel 化合物将显示于面板上化合物将显示于面板上 GRAPH is the default view图形图形是默认是默认查看查看Dis

44、plays the chromatogram during the run 显示运行过程中的色谱图显示运行过程中的色谱图 Displays:显示显示: Compound Name 化合物名化合物名 Retention Time 滞留时间滞留时间 TIC TIC MaxCopyright 200937CMPD ID View CMPD ID 查看查看 The data can be viewed as a list by touching CMPD ID during a run 在运行过程中在运行过程中, 按按 CMPD ID 可查看数据清单可查看数据清单 Displays:显示显示: Com

45、pound Name 化合物名化合物名 CAS Number CAS 数数 Retention Time 滞留时间滞留时间 Net Fit 纯拟合纯拟合Copyright 200938Purge Headspace 冲净顶空冲净顶空 A prompt will appear to purge at the end of each run 每次运行终点每次运行终点, 将出现冲净提示将出现冲净提示 To purge, insert the Headspace needle into the purge vial 为冲净为冲净, 将顶空针头插入冲净瓶中将顶空针头插入冲净瓶中 Average purg

46、e time is 2 minutes 平均冲净时间为平均冲净时间为 2 分钟分钟 Cleans residual sample from the needle 从针头中清除残余的样品从针头中清除残余的样品 Touch ContinueCopyright 200939Method Finished 方法完毕方法完毕 When the method and purge are complete, METHOD FINISHED will be displayed 当方法和冲净完成时当方法和冲净完成时, 将显示将显示方法完毕方法完毕字样字样Copyright 200940HSS Blank fro

47、m the Smart Plus 来自来自 Smart Plus 的的 HSS 空样空样 Chlorobenzene 氯苯氯苯-d5 (7:30)Fluorobenzene 氟苯氟苯 (2:50)1,4-Dichlorobenzene 二氯苯二氯苯 -d4 (14:30)Bromopentafluorobenzene 氟溴苯氟溴苯 (8:00 +/- 0:10)Copyright 200941HSS Blank from the ER 来自来自 ER 的的 HSS 空样空样Fluorobenzene 氟苯氟苯 (1:30) Chlorobenzene 氯苯氯苯-d5 (3:35)1,4-Dic

48、hlorobenzene二氯苯二氯苯 -d4 (6:45)Bromopentafluorobenzene 氟溴苯氟溴苯 (3:45 +/- 0:05)Copyright 200942Exercise: Preparing a HSS Sample 练习练习: 准备准备 HSS 样品样品 Use a 40 mL vial to prepare the sample用用40 毫升样瓶准备样品毫升样瓶准备样品 Add 20 mL VOC-free water 添加添加 20 毫升无毫升无 VOC 水水 Inject 1 m mL HSS Internal Standard 注入注入 1 微升微升 H

49、SS 内部标准内部标准 Inject 1 m mL Sample 注入注入 1 微升样品微升样品 Place the sample in the HSS and allow to equilibrate for 20 minutes 将样品置入将样品置入 HSS, 并让它平衡并让它平衡 20 分钟分钟 Run Method 运行方法运行方法 ER_HSS_Tri-Bed_PPB_Quant Select and use the Quant tab to view data 选用选用 Quant 标签条查看数据标签条查看数据Copyright 200943Worksheet: HSS Blank

50、工作表工作表: HSS 空样空样Copyright 200944Accessing Quantitation Reports 查访定量报告查访定量报告 Double-click on the ID Unknowns icon 双击双击 ID 未知物未知物 图标图标 The user is prompted to select the method type 提示用户选方法类型提示用户选方法类型 Select GC/MS 选选 GC/MS Click OK 单击单击 OKCopyright 200945Accessing Quantitation Reports 查访定量报告查访定量报告 Sel

51、ect HAPSITE 选选 HAPSITE Double-click on the desired method 双击所需的方法双击所需的方法Copyright 200946Accessing Quantitation Reports 查访定量报告查访定量报告 Click Browse to select the desired Data File 单击单击 浏览浏览 选所需的选所需的数据文件数据文件 The pathway for the data file: 数数据文件的途径据文件的途径: Click Start 单击单击 开始开始Copyright 200947Quantitation

52、 Report 定量报告定量报告Compounds Detected检测的化合物检测的化合物Retention Time 滞留时间滞留时间 Concentration 浓度浓度 微克微克/升升 (ppb)Copyright 200948Accessing Quantitation Reports 查访定量报告查访定量报告 ID Unknowns will label the peak of each chemical from the calibration library with a “T” ID 未知物未知物 将用将用 “T” 标志来自校准库的每个化合物的峰标志来自校准库的每个化合物的峰

53、Copyright 200949Quantitation Reports 定量报告定量报告 Use caution when using Quantitation reports 当用定量报告时当用定量报告时, 使用警示使用警示The report identifies and quantifies only the chemicals that are specifically referenced in the calibration library 报告鉴别和定量仅对校准库中特定引用的化合物报告鉴别和定量仅对校准库中特定引用的化合物The chromatogram must be vis

54、ually inspected for additional peaks that were not identified in the report 必须视觉检查色谱图是否存在报告中未鉴别的附加峰必须视觉检查色谱图是否存在报告中未鉴别的附加峰Copyright 200950Quantitation Reports 定量报告定量报告 ID the unidentified peak(s) using: ID 未鉴别峰用未鉴别峰用: AMDIS Search AMDIS 搜索搜索 Automatic NIST Search 自动自动 NIST 搜索搜索 Manual NIST Search 手动

55、手动 NIST 搜索搜索 Estimate the concentration 估算浓度估算浓度Copyright 20095112.5 ppb(25 if using BPFB) HSS Concentration Estimates This formula is used to estimate concentration of chemicals based on the internal standardsNOTE: This calculation is for a concentrator method using a 1 L internal standard injectio

56、nMax normalization value of nearest internal standardMax normalization value of compound of interestXApproximate Concentration ( +/- one order of magnitude)Max normalization TCE (15,900,000)Max normalization fluorobenzene (7,000,000)12.5 ppb(2.8 to 280 ppb)=X28 ppb=Copyright 20095212.5 ppb(25 if using BPFB) HSS 浓度估算浓度估算 本式用于估算基于内部标准的化合物浓度本式用于估算基于内部标准的化合物浓度注注: 本计算用于浓缩器方法本计算用于浓缩器方法, 用用 1 微升内部标准注入微升内部标准注入内部标准的内部标准的最大归一化值