多肽合成蛋白测序

1、多肽合成蛋白测序433多肽合成系统公认的经典高效全自动合成系统l         合成规模0.11.0 mmol，可采用Fmoc和tBoc两种方法l         特有的涡流混合式反应腔和NMP溶剂系统：活化氨基酸与肽充分反应，偶联率高达99以上l         专利的零死体积阀门设计：去除交叉污染，减少试剂消耗l   &#

2、160;     全套完备的试剂和树脂l         全自动电脑控制，程序编辑快速简便l         特有回馈监控功能，自动强化脱保护和偶联，保证高得率491型蛋白质测序仪Procise 系列HT和cLC，型蛋白测序仪l         独一无二的高灵敏高效率蛋白序列测定系统l   

3、;      适于各种方法制备样品的测序：电转移印迹法、PVDF膜上样品或HPLC等溶液样品，还适于各种蛋白的测序l         全自动电脑监控：对于未知蛋白测序编辑新程序即可，并可同步监测反应情况随时调整以获得最佳结果，便于GMP控制l         精确的试剂输送回馈调节系统：使试剂输送精确度，化学反应效率、灵敏度提高，达fmol级样品l   &#

4、160;     独立的高精度HPLC系统分析PTH氨基酸l Procise C 为端测序系统  173毛细管HPLC及微量蛋白印迹制备系统 l         一体化的超微量高效液相色谱仪，样品分离收集一步完成l         灵敏度高，回收率高，可用于500fmol蛋白/多肽样品制备l      

5、60;  自动收集样品到PVDF膜上，避免了人为的误差l         PVDF膜可直接用于蛋白测序仪：可与质谱仪联用l         样品斑点只有23mm，无需进一步浓缩或转移，减少了样品的丢失及污染可能l 检测器灵敏度极高，专适于超微量分析和微量制备        多肽合成试剂试剂 分子量 数量 产品编号DCM 84.9 1000 ml G

6、EN902017DIPEA (density 0.742 g/mL) 129.2 4 x 9 ml GEN075000DMAP 122.2 1 g GEN910015DMF (Peptide synthesis grade, low amine content, 2 ppm)(25 L DMF available on request) 122.2 1000 ml GEN910003 2500 ml GEN002007Fmoc-succinimidyl carbonate (For introduction of Fmoc group) 337.3 5 g GEN91001620% (v/v)

7、 Piperidine/DMF 1000 ml GEN910008TFA 100 ml GEN910004脱保护试剂 (20% (v/v) Piperidine(DMF) 4 升GEN903081DIPEA 1 升GEN903082DMF 4 升GEN903080DMF 25 升 GEN002507活化试剂DIPCDI (density 0.806g/ml) 126.2 25 ml GEN910006HATU 380.2 5 g GEN076521 25 g GEN076523100 g GEN076525 1000 g GEN076527 HOAt 136.1 5 g GEN07651125

8、 g GEN076513 100 g GEN076515 1000 g GEN076517 HOBt · H2O 153.1 5 g GEN07600125 g GEN076003 100 g GEN076005 PyAOP 521.4 5 g GEN07653125 g GEN076533 TBTU 321.1 5 g GEN030003Linker A-Opfp348.2 1 g GEN910017Linker A 182.2 1 g GEN910009 Linker B-Opfp 318.2 1 g GEN910018Linker B 152.2 1 g GEN910010Li

9、nker H-Opfp 378.3 1 g GEN910019 Linker H 212.3 1 g GEN910011433A 试剂401466 Synergy Reagent Kit (100 cycles) Includes: 2 Piperidine (P/N 400629), 2 THF (P/N 401255), 2 DMF (P/N 400143), 1 HBTU (P/N 401278), 1 HOBt/DMSO/NMP (P/N 401279), and 1 DIEA/DMSO/NMP (P/N 401254). 400629 Piperidine, 200 mL 40125

10、5 THF, 200 mL 400143 DMF, 4 L 401278 HBTU, 8 mmoles 401279 HOBt/DMSO/NMP, 0.2 M, 40 mL 401254 DIEA/DMSO/NMP, 40 mL Fmoc-L 氨基酸产品 分子量 包装 产品号Ala-OH 311.3 5 g GEN911011 25 g GEN911012 100 g GEN758002-Ala-OH 311.3 5 g GEN911071Aib-OH 325.4 5 g GEN911072 25 g GEN911073Arg-OH 396.4 5 g GEN911013Arg-(Pbf)-O

11、H 648.8 5 g GEN911096 25 g GEN911097 100 g GEN911098Asn-OH 354.4 5 g GEN911017 25 g GEN911018 100 g GEN758004Asn(Trt)-OH 596.7 1 g GEN911016 5 g GEN911019 100 g GEN758178Asp(OtBu)-OH 411.5 5 g GEN911020 25 g GEN911021 100 g GEN758005Cys(Acm)-OH 414.5 1 g GEN911023 5 g GEN911024Cys(tBu)-OH 399.5 5 g

12、GEN911029 25 g GEN911030Cys(StBu)-OH 431.6 5 g GEN911031Cys(Trt)-OH 585.7 5 g GEN911026 25 g GEN911027 100 g GEN758022Gln-OH 368.4 5 g GEN911032 25 g GEN911033 100 g GEN758012Gln(Trt)-OH 610.7 1 g GEN911034 5 g GEN911037 100 g GEN758179Glu(OtBu)-OH 425.5 5 g GEN911035 25 g GEN911036 100 g GEN758021G

13、ly-OH 297.3 5 g GEN911038 25 g GEN911039 100 g GEN758013His(Trt)-OH 619.7 5 g GEN077213 25 g GEN077215 100 g GEN758183His(Fmoc)-OH 599.6 5 g GEN911041 25 g GEN911042 100 g GEN758111Ile-OH 353.4 5 g GEN911044 25 g GEN911045 100 g GEN758014Leu-OH 353.4 5 g GEN911047 25 g GEN911048 100 g GEN758015Lys(F

14、moc)-OH 590.7 1 g GEN911093 5 g GEN911094 25 g GEN911095Lys(tBoc)-OH 468.6 5 g GEN911050 5 g GEN911051 100 g GEN758006Met-OH 371.5 5 g GEN911053 25 g GEN911054 100 g GEN758016Nle-OH 353.4 1 g GEN077301 25 g GEN911055 100 g GEN758023Orn(tBoc)-OH 454.5 5 g GEN911056Phe-OH 387.4 5 g GEN911057 25 g GEN9

15、11058 100 g GEN758017Pro-OH 337.4 5 g GEN911059 25 g GEN911060 100 g GEN758018Ser(tBu)-OH 383.4 5 g GEN911061 25 g GEN911062 100 g GEN758009Thr(tBu)-OH 397.5 5 g GEN911063 25 g GEN911064 100 g GEN758010Trp-OH 426.5 5 g GEN911065 25 g GEN911066 100 g GEN758019Trp(tBoc)-OH 526.6 1 g GEN911090 5 g GEN9

16、11091 25 g GEN911092Tyr(tBu)-OH 459.5 5 g GEN911067 25 g GEN911068 100 g GEN75801Val-OH 339.4 5 g GEN911069 25 g GEN911070 100 g GEN758020烯丙基（Allyl ）保护的Fmoc 氨基酸烯丙基保护的Asp 和 Glu 主要用以合成环化和侧链多肽，以及磺化，糖基化，磷酸化肽链Asp(OAl)-OH 395.4 5 g GEN91120525 g GEN911206Asp(OH)-Oal 395.4 1 g GEN9113005 g GEN91130125 g GE

17、N911302Glu(OAl)-OH 409.4 5 g GEN91120725 g GEN911208Glu(OH)-Oal 409.4 1 g GEN9113055 g GEN91130625 g GEN911307Lys(Aloc)-OH 452.5 5 g GEN91120925 g GEN911210Tyr(Al)-OH 443.5 5 g GEN91121125 g GEN911212Fmoc-D-氨基酸Ala-OH311.31 g GEN911105Asn-OH354.4 1 g GEN911109Asp(OtBu)-OH 411.51 g GEN911111Gln-OH 368

18、.41 g GEN911113Glu(OtBu)-OH425.5 1 g GEN911115His(Trt)-OH 619.7 1 g GEN911108Ile-OH353.4 1 g GEN911128Leu-OH 353.4 1 g GEN911117Lys(tBoc)-OH 468.6 1 g GEN911119Met-OH 371.5 1 g GEN911121Nle-OH 353.4 1 g GEN911123Phe-OH 387.4 1 g GEN911126Pro-OH337.4 1 g GEN911124Ser(tBu)-OH 383.4 1 g GEN911138Thr(tB

19、u)-OH 397.5 1 g GEN911139Trp-OH 426.5 1 g GEN911132Tyr(tBu)-OH 459.5 1 g GEN911140Val-OH 339.4 1 g GEN911136-Fmoc-L-氨基酸OPfps带OPfp 脂的活化氨基酸主要用于HOBt 或 HOAt 等催化剂作用Ala-Opfp 477.4 5 g GEN91201125 g GEN912060Asn-Opfp 520.4 5 g GEN91201325 g GEN912061Asn(Trt)-Opfp 762.7 1 g GEN9120085 g GEN912009Asp(OtBu)-O

20、pfp 577.5 5 g GEN91201525 g GEN912062Cys(Acm)-Opfp 580.6 1 g GEN9120195 g GEN91202025 g GEN912063Cys(tBu)-Opfp 565.6 1 g GEN9120235 g GEN912024Cys(Trt)-Opfp 751.8 5 g GEN91202225 g GEN912064Gln-Opfp 534.4 5 g GEN91202625 g GEN912065Gln(Trt)-Opfp 776.8 5 g GEN91208125 g GEN912082Glu(OtBu)-Opfp 591.5

21、5 g GEN91202825 g GEN912066Gly-Opfp 463.4 5 g GEN91201825 g GEN912067His(tBoc)-Opfp 643.6 5 g GEN91203025 g GEN912068Ile-Opfp 519.5 5 g GEN91203225 g GEN912069Leu-Opfp 519.5 5 g GEN91203425 g GEN912070 Lys(tBoc)-Opfp 634.6 5 g GEN91203625 g GEN912071Met-Opfp 537.5 5 g GEN91203825 g GEN912072Nle-Opfp

22、 519.5 5 g GEN912054Phe-Opfp 553.5 1 g GEN9120405 g GEN91204125 g GEN912073Pro-Opfp 503.4 5 g GEN91204325 g GEN912074Ser-(tBu)-Opfp 549.5 5 g GEN91208725 g GEN912088Thr(tBu)-Opfp 563.5 5 g GEN91209025 g GEN912091Trp-Opfp 592.5 5 g GEN91204925 g GEN912077Tyr(tBu)-Opfp 625.6 5 g GEN91205125 g GEN91207

23、8Val-Opfp 504.4 5 g GEN91205325 g GEN912079多肽合成相关文献Reviews and mini-reviews1. “Instrumentation for automated solid-phase peptide synthesis.” Cammish，L.E.，Kates，S.A. In Fmoc Solid Phase Peptide Synthesis: A Practical Approach (Chan，W.，White，P.) Oxford University Press，Oxford，1998，in press.2. “Peptide

24、 technologies: Nuts & bolts intelligence on practical industry solutions for peptede design，purification and production.” Kates，S.A. Current Drugs Ltd，1998，in press.3. “Functionalized resins and linkers for solid-phase synthesis of small molecules.” Blackburn，C.，Albericio，F.，Kates，S.A. Drugs of

25、the Future，1997，22，1007.4. “Polyethylene glycol-containing supports for solid-phase synthesis of peptides and combinatorial organic libraries.” Barany，G.，Albericio，F.，Kates，S.A.，Kempe，M. In Chemistry and Biological Application of Polyethylene Glycol，ACS Symposium Series (Harris，J.M.，Zalipsky，S.，Eds.

26、) American Chemical Society Books，Washington，DC，1997，239.5. “Solid-phase peptide and small molecule synthesis.” Blackburn，C.，Albericio，F.，Kates，S.A. In Recent Research Developments in Organic Chemistry (Pandalai，S.G.，Ed.) Transworld Research Network，India 1997，1，477. 6. “Solid-phase synthesis of cyc

27、lic homodetic peptides.” Blackburn，C.，Kates，S.A. In Methods of Enzymology (Fields，G.B.，Ed.) Academic Press，New York，1997，289，175.7. “N-(dimethylamino)-1H-1,2,3-triazolo4,5-bpyridin-1-ylmethylene-N-methylmethan-aminium hexafluorophosphate N-oxide.” Albericio，F.，Kates，S.A.，Carpino，L.A. In Encyclopedia

28、 of Reagents for Organic Synthesis (Paquette，L.A.，Ed.) Wiley， New York，1996，3，2026.8.“3-Hydroxy-3H-1,2,3- triazolo4,5-bpyridin.” Kates，S.A.，Albericio，F，Carpino，L.A. In Encyclopedia of Reagents for Organic Synthesis (Paquette，L.A.，Ed.) Wiley， New York，1996，4，2784.9. “Azidotris(dimethylamino)phosphoni

29、um hexafluorophosphaste.” Albericio，F.，Kates，S.A. In Encyclopedia of Reagents for Organic Synthesis (Paquette，L.A.，Ed.) Wiley， New York，1996，1，224.10. “1,1-Axobis-1-cyclohexanenitrile(ACN).” Kates，S.A.，Albericio，F. In Encyclopedia of Reagents for Organic Synthesis (Paquette，L.A.，Ed.) Wiley， New York

30、，1996，1，228.11. “1-Phenylsulfonyl-1H-tetrazole.” Eritja，R.，Kates，S.A.，Albericio，F. In Encyclopedia of Reagents for Organic Synthesis (Paquette，L.A.，Ed.) Wiley， New York，1996，6，4058.12. “1-benzotriazolyl diethyl phosphate.” Albericio，F. Kates，S.A. In Encyclopedia of Reagents for Organic Synthesis (Pa

31、quette，L.A.，Ed.) Wiley， New York，1996，1，300.13. “O-Benzoriazol-1-yl-N,N,N,N-tetramethyluronium hexafluorophosphate (HBTU)”. Albericio，F.，Kates，S.A. In Encyclopedia of Reagents for Organic Synthesis (Paquette，L.A.，Ed.) Wiley， New York，1996，1，304.14. “4,4-Bis(2-amino-6-methylpyrimidyl) disulfide.” Alb

32、ericio，F.，Kates，S.A.，Eritja，R. In Encyclopedia of Reagents for Organic Synthesis (Paquette，L.A.，Ed.) Wiley， New York，1996，1，421.15. “Solid-phase synthesis of cyclic peptides.” Kates，S.A.，Solé，N.A.，Albericio，F.A.，Barany，G. In Peptides: design，synthesis and biological activity (Basava，C.，Ananthar

33、amaiah，G.M.Eds.) Birkhauser，Boston(Massachusetts)，1994，39.16. “Synthetic Peptides Come of Age.” Fishman，J.B.，Kates，S.A. Biomedical Products Review，1995，20，52.Article in refereed journals1. “Continuous-flow solid-phase peptide sysnthesis using polystryrene resins.” Kates，S.A.，Cammish，L.E.，Albericio，F

34、.J. Peptide Science，1998，in press.2. “On the uniformity of product quality for peptides generated in service laboratories: Peptides containing phosphorylated tyrosone residues.” Bonewald，L.F.，Bibbs，L.，Kates，S.A.，Khatri，A.，McMurray，J.S.，Medzihradszky，K.F.，Weintraub，S.T. J. Peptide Science，1998，in pre

35、ss.3. “Improved synthesis and purification of Alzeimers Ab 1-42 and analogs.” Milton，S.C.F.，de Lisle Milton，R.C.，Kates，S.A.，Glabe，C. Letters in peptide Science，1998，in press.4. “High load” Polyethylene glycol-polystryrene (PEG-PS) graft supports for solid-phase synthesis.” Kates，S.A.，Blackburn，C.，Mc

36、Guinness，B.F.，Griffin，G.W.，Solé，N.A.，Barany，G.，Albericio，F. Biopolymers: Peptide Science，1998，in press.5. “On the Use of Onium Salt-Based Coupling Reagents in Peptide Synthesis” Albericio，F.，Bofill，J.M.，Carpino，L.A.，El-Faham，A.，Kates，S.A. J.Org. Chem.1998，in press.6. “Automated，high productivit

37、y purification and analysis of synthetic peptides.” Londo，T.R.，Kehoe，T.，Kates，S.A.，Hantman，S.，Gordon，N.F. Letters in Peptide Science，1998，in press.7. “On the use of PyAOP，a phosphonium salt derived from HOAt，in solid-phase peptide synthesis.” Albericio，F.，Cases，M.，Alsina，J,.，Triolo，S.A.，Carpino，L.A.

38、，Kates，S.A. Tetrahedron Lett.，1997，38，4853.8. “N-Dependent transcriptional antitermination in phage is mediated by an inducible RNA bridge.” Su，L.，Radek，J.T.，Labeots，L.A.，Hermanto，P.，Chem，H.，Nakagawa，S.，Zhao，M.，Kates，S.A.，Weiss，M.A. Genes & Development，1997，11，2214.9. “Optimization of the synthe

39、sis of peptide combinatorial libraries (SPCLs) using a one-pot method.” Herman，L.W.，Tarr，G.，Kates，S.A. Molecular Diveristy，1996，1，147. 10. “Solid-phase synthesis of angiotensin converting enzyme (ACE) inhibtor libraries derived from N-alkylated dipeptide.” Blackburn，C.，Pingali，A.，Kehoe，T.，Herman，L.W

40、.，Wang，H.，Kates，S.A. Bioorg. Chem. Med. Lett.1997，7，823.11. “N-Piperidylalanine formation during the preparation of C-terminal Cys peptides with an Fmoc/tBu strategy.” Lukszo，J.，Patterson，D.，Albericio，F.，Kates，S.A. Lett. Peptide Science，1996，3，157.12. “Labeling of synthetic biomolecules with biotin

41、and fluorescein by active esters of 1-phenylpyarzolin-5-ones.” Kremsky，J.N.，Pluskal，M.，Casey，S.，Perry-OKeefe，H.，Sinha，N.D.，Kates，S.A. Tetrahedron Lett. 1996，37，4313.13. “Preparation and applications of xanthenylamide (XAL) handles for solid-phase synthesis of C-terminal peptide amides under particul

42、arly mild conditions.” Han，Y,.，Bontems，S.L.，Hegyes，P.，Munson，M.C.，Minor，C.A.，Kates，S.A.，Albericio，F.，Barany，G. J. Org. Chem.，1996，61，6326.14. “Optimized preparation of deca(L-alanyl)-L-valinamide by 9-fluorenylmethyloxycarbonyl (Fmoc) Solid-phase synthesis on polyethylene glycol-polystyrene (PEG-PS)

43、 graft supports，with 1,8-diazobicyclo5.4.0-undec-7-ene (DBU) deprotection.” Kates，S.A.，Solé，N.A.，beyermann，M.，Barany，G.，Albericio，F. Peptide Res.，1996，9，106. 15. “Novel carbozylic acid and carboxamide protective groups based on the exceptional stablization of the cyclopropylmethyl cation.” Carp

44、ino，L.A.，Chao，H.-G.，Ghassemi，S.，Mansour，E.M.E.，Riemer，C.，Warrass，R.，Sadat-Aalaee，D.，Truran，G.A.，Imazumi，H.，El-Faham，A.，Ionescu，D.，Ismail，M.，Kowaleski，T.L.，Han，C.H.，Wenschuh，H.，Beyermann，M.，Bienert，M.，Shroff，H.，Alericio，F.，Triolo，S.A.，Solé，N.A.，Kates，S.A，J. Org. Chem.，1995，60，7718.16. “Rearrangement of Glu(OtBu) and Asp(OtBu)-containing peptides upon fluoride treatment in solid-phase synthesis.” Kates，S.A.，Alberi