芩丹胶囊论文：芩丹胶囊高血压自发性高血压大鼠外膜重构Ⅰ、Ⅲ胶原

1、构I、皿胶原【中文摘要】1.观察苓丹胶囊对自发性高血压大鼠血压的影响。2.观察苓丹胶囊对血管外膜I、皿胶原表达的影响，并探讨苓丹胶囊逆转高血压血管外膜重构的机制。方法1.研究对象：32只40周龄雄性SHF大鼠随机分为4组,模型组（SHR组）、苓丹胶囊大剂量组（SHR+QD组）、苓丹胶囊小剂量组（SHR+QD组）、氯沙坦组（SHR+Los组）,另设40周龄雄性WKY大鼠16只分为空白对照组（WKY空白组）及正常药物对照组（WKY+Q组）。SHR+Q给予苓丹胶囊750mg/(kgd);SHR+QD组给予苓丹胶囊150mg/(kgd);SHR+Los组给予氯沙坦30mg/(kgd);WKY+750m

2、g/(kg-d);WKY白组和SHF组给予相等剂量生理盐水,1次/d,连续给药12周各组大鼠在末次给药后禁食24h,不禁水。10%水合氯醛腹腔注射麻醉后进行后续实验。2.研究内容：（1）大鼠尾动脉收缩压测定。（2）免疫组化法观察胸主动脉外膜I皿胶原表达。（3）实时定量PCR佥测胸主动脉外膜I、皿胶原表达。用westernblotting检测I胶原蛋白表达。结果1.各组大鼠治疗前后收缩压比较见图1,从给药第4周起,SHR+QD组、SHR+QD组、SHR+Lo组分别与SHR1比较,血压下降明显，其差异均具有统计学意义（P0.05）,SHR+QDH组与SHR+QDL组比较差异有统计学意义（P0.05

3、）。3.主动脉壁I、皿胶原mRNA表达水平比较采用2-Ct方法，将I、皿型胶原基因表达Ct值转换为相对于WKY空白组的变化倍数，可以直接得到的基因相对于参照基因的定量。如图6和7所示分别为I、皿胶原的扩增、溶解曲线和溶解峰，说明I、皿胶原是特异性基因表达，无引物二聚体及非特异性扩增。研究结果显示：见图4,WKY空白组和WKY+1间差异无统计学意义(P0.05),SHR+QDH组和SHR+QD组间差异有统计学意义(P0.05),SHR+QDH组和SHR+QDfi间差异有统计学意义(P0.05)。结论芩丹胶囊对SHR大鼠有显著的降压疗效，其降压机制与芩丹胶囊降低SHR大鼠I、皿胶原表达水平有关，同

4、时与逆转血管外膜重构，抑制外膜纤维化，减少I、皿胶原在外膜的合成与沉积有关。【英文摘要】Aiml.ObserveQindancapsuleonbloodpressureinspontaneouslyhypertensiverats.2.ObservetheQindancapsuleonvascularadventitiaI,皿collagenexpression,andtoexplorethereversalofhypertensivevascularadventitiaremodelingmechanism.Methods1.Study:32SHRratsof40weeksoldwerera

5、ndomlydividedinto4groups,themodelgroup(SHRgroup),Qindanhighdosagegroup(SHR+QDHgroup),Qindanlowdosagegroup(SHR+QDLgroup),theLosartangroup(SHR+Losgroup),Besides,16WistarKyoto(WKY)ratsofthesameweeksweretakenasthenormalcontrol(WKYfcontrolgroup)andQindangroup(WKY+QDgroup).SHR+QDHgroupwastreatedwithhighdo

6、sage750mg/(kgd);SHR+QDgroupwastreatedwithlowdosage150mg/(kgd);SHR+Losgroupwasgivenlosartan30mg/(kgd);WKY+QDgroupweretreatedwithhighdosage750mg/(kgd);WKYandSHRcontrolgroupweregivenanequaldosageofsaline,onceaday,continuousadministrationfor12weeks.Ratsineachgroupafterthelastadministrationinthefasted24h

7、,cannothelpbutwater.Alltheratswereanesthetizedwithintraperitonealinjectionof10%chloralhydratefollow-upexperiments.2.Research:。)Determinationofrattailarterysystolicpressures.(2)ObservedaorticadventitiaI,皿collagenbyimmunohistochemistry.(3)Real-timequantitativePCRdetectionofthoracicaorticadventitiaI,皿c

8、ollagen.(4)WesternblottingdetectionofexpressionofIcollagen.Results1.ComparisonofsystolicbloodpressureofratsFig1,after4weeksfromtheadministration,Qindanhighdosage,lowdosagegroup,andlosartangroupcomparedwiththemodelgroupbloodpressuredecreased,thediffereneewasstatisticallysignificant(P0.05),Qindanhighdosagegroupcomparedwiththelowdosagegroupwasstatisticallysignificant(P0.05).3.AorticwallI,皿collagenmRNAexpressioncomparedUsing2-Ctmethod,typeI,皿collagengeneexpressionrelat