常用凝胶参数

1、常用凝胶G技术参数1.葡聚糖凝胶内某些技术数据种类干颗粒直径小分子量分级范围床体积毫升/克干分子筛得水值溶胀最少平衡时间h柱头压力kPa2.5cm直径柱肽及球形蛋白质葡聚糖线性分子室温沸水浴SephadexG-1040120700700231.00.131SephadexG-1040120150015002.53.51.53.531SephadexG-25粗级100300(=5100目)中级50150(-100200目)1,0005,0001005,000461.50.262细级20800(弋200400目)超细1040SephadexG-50粗级100200中级501501,500500细级

2、208030,00010,0009115.00.362超细1040SephadexG-75401203,0001,000超细104070,000950,00012157.50.52433.9215.86SephadexG-100401204,0001,000超细10401,500,00050,000152010.01.04852.359.41SephadexG-150401205,0001,0002030超细1040400,000150,000182215.01.57250.883.53SephadexG-200401205000100030401040800,000200,000202520

3、.02.07250.391.572.聚丙烯酰胺凝胶CD技术数据型号排阻内下限分子量分级别离范围分子量膨胀后内床体积ml/g干凝胶膨胀所需最少时间室温,hBio-gel-P-21,6002002,0003.824Bio-gel-P-43,6005004,0005.824Bio-gel-P-64,6001,0005,0008.824Bio-gel-P-1010,0005,00017,00012.424Bio-gel-P-3030,00020,00050,00014.91012Bio-gel-P-6060,00030,00070,00019.01012Bio-gel-P-100100,00040,0

4、00100,00019.024Bio-gel-P-150150,00050,000150,00024.024Bio-gel-P-200200,00080,000200,00034.048Bio-gel-P-300300,000100400,00040.0483.琼脂糖凝胶内技术数据型号琼脂糖含量%W/vy排阻内下限分子量分级别离内范围分子量生产厂家Sepharose4B40.3X1063X106PharmaciaSepharose2B22X10625X106Sagavac10102.5X1051X1042.5X105SeravacSagavac887X1052.5X1047X105Sagava

5、c662X1065X10-2X106Sagavac4415X1062X10515X106Sagavac22150X1065X10515X107Bio-gelA-0.5M100.5X1061X104-0.5X106Bio-gelA-1.5M81.5X106密子第位1端CUALeuCCAProCAAGlnCGAArgACUGLeuCCGProCAGGlnCGCArgGAUUIleACUThrAAUAsnAGUSerAUCIleACCThrAACAsnAGCSerqAAUAIleACAThrAAALysAGAArgAAUGMetACGThrAAGLysAGCArgGUUValGCUAlaGAUAsp

6、GGUGly口GUCValGCCAlaGACAspGGCGlyGGUAValGCAAlaGAAGluGGAGlyAGUGValGCGAlaGAGGluGGGGly目九、常用酸碱技术参数1.常见内市售酸碱内浓度溶质分子式分子量mol/Lg/L重量(%比重配制mol/L溶液内参加量(ml/L)冰乙酸CHCOOH60.0517.40104599.51.05057.5乙酸60.056.27376361.04559.5甲酸HCOOH46.0223.40080901.200z工2.7盐酸HCl36.5011.604124361.18036.22.90105101.050344.8硝酸HNO63.0215.

7、991008711.42062.514.90938671.40067.113.30f337611.37075.2高氯酸HclOa100.5011.651172701.67085.89.20923601.540108.7磷酸HPO80.0018.101445851.70055.2硫酸H2PQ98.1018.00776961.84055.6多氧化钱NHOH35.0014.80:?51280.89857.6多氧化钾KOH56.1013.507节7501.52074.11.9410910II1.090515.5多氧化钠NaOH40.0019.10,63501.53052.42.75111101.110363.42.各种浓度CD酸碱贮存液CD近似pH值溶质1N30.1Na0.01Na0.001Na乙酸0.402.903.403.90盐酸0.101.072.023.01硫酸0.