Cresyl-Violet-acetate _Dye Reagent - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECresyl Violet acetateCat. No.: HY-101888CAS No.: 10510-54-0分式: CHNO分量: 321.33作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 1 mg/mL (3.11 mM; Need ultrasonic)H2O : 0.

2、67 mg/mL (2.09 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.1121 mL 15.5603 mL 31.1207 mL5 mM - - -10 mM - - -请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Cresyl Violet acetate种红荧光染剂，可于染神经元。体外研究The estimated total number of SG neurons is 27,4853251 a

3、nd 26,7051823 in the PV and Cresyl Violetacetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore,Cresyl Violet acetate staining is simpler and more cost effective when estimates neuronal number. AlthoughPV stains spiral ganglion neurons (SGNs) wit

4、h a greater intensity and provides a functional status, its tedious1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEprotocol limits its use for quantification. Total RC volume is estimated using probe and it is found that anaverage RC volume of 2.1620.35 mm3 and 1.820.33 mm3 in Cresyl Violet acetate

5、 staining and PVimmunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is3487.63951 m3 and 3740.1784 m3 in Cresyl Violet acetate staining and PV immunostaining sections,respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator p

6、robe and it is found tobe 131.6850 m3 and 126.5133 m3 in Cresyl Violet acetate staining and PV immunostaining sections,respectively 1.PROTOCOLCell Assay 1 Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4C.The sections are then used for Cresyl vi

7、olet acetate and immunohistochemical (IHC) staining. Every 7thsection is stained with Cresyl violet acetate (1%), dehydrated with ascending grades of alcohol, cleared withxylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl violet acetatestaining sections from each spec

8、imen are used for stereology. None of these cases show anyhistopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal(RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus(nucleator probe) is done with soft

9、ware 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positiveneurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.McePdfHeightCaution: Product has not been fully validated for me