Dabigatran-ethyl-ester-hydrochloride - Thrombin 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDabigatran ethyl ester hydrochlorideCat. No.: HY-77521CAS No.: 211914-50-0分式: CHClNO分量: 536.03作靶点: Thrombin作通路: Metabolic Enzyme/Protease储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 50 m

2、g/mL (93.28 mM)H2O : 5 mg/mL (9.33 mM; Need ultrasonic)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.8656 mL 9.3278 mL 18.6557 mL5 mM 0.3731 mL 1.8656 mL 3.7311 mL10 mM 0.1866 mL 0.9328 mL 1.8656 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验

3、动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (4.66 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (4.66 mM);

4、 Clear solution1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (4.66 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Dabigatran ethyl ester hydrochloride有效地核糖 氢烟酰胺脱氢酶(NQO2)抑制剂，IC50值为0.8 M；也 凝酶的抑制剂。IC50 & Target IC50: 0.8 M (NQO2) 1体外研究 The Ki of da

5、bigatran (ethyl ester hydrochloride) toward NQO2 is 0.9 M and the IC50 is 0.8 M. The ethylester group of dabigatran (ethyl ester hydrochloride) significantly extends the interaction surface especiallywith hydrophobic amino acids such as Ile 128 and Met 154. Dabigatran ethyl ester has higher affinity

6、 thanDabigatran to both thrombin and NQO2 1. Dabigatran is a highly selective, reversible, and potent thrombininhibitor and is orally available as the prodrug, dabigatran etexilate 2.体内研究 Dabigatran (Ki=4.5 nM) could bind to human thrombin selectively, and reversibly to realize a strong and long-las

7、ting anticoagulant effect 3.PROTOCOLKinase Assay 1 NQO2 (0.5 M) is incubated with the substrate mitomycin C (50 M) and four different Dabigatranconcentrations in 100 mM potassium phosphate buffer (pH 5.8) at room temperature for 5 min prior to theaddition of NADH (in increasing concentrations) as a

8、cosubstrate and photometric monitoring at 340 nm for30 min at rt. Ki values are determined. Data generated are used to calculate the IC50 of inhibition of NQO2activity 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Biochem Pharmacol. 2016

9、 Nov 1;119:76-84.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Michaelis S, et al. Dabigatran and dabigatran ethyl ester: potent inhibitors of ribosyldihydronicotinamide dehydrogenase (NQO2). J MedChem. 2012 Apr 26;55(8):3934-44.2. Eisert WG, et al. Dabigatran: an oral novel

10、potent reversible nonpeptide inhibitor of thrombin. Arterioscler Thromb Vasc Biol. 2010Oct;30(10):1885-9.3. Hauel NH, et al. Structure-based design of novel potent nonpeptide thrombin inhibitors. J Med Chem. 2002 Apr 25;45(9):1757-66.McePdfHeight2/2 Master of Small Molecules 您边的抑制剂师www.MedChemECaution: Product has not been fully valid