Sardomozide-dihydrochloride-CGP-48664A-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESardomozide dihydrochlorideCat. No.: HY-13746BCAS No.: 138794-73-7Synonyms: CGP 48664A分式: CHClN分量: 303.19作靶点: Others作通路: Others储存式: Powder -20C 3 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 25 mg/mL (82.46 mM; Need

2、 ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.2983 mL 16.4913 mL 32.9826 mL5 mM 0.6597 mL 3.2983 mL 6.5965 mL10 mM 0.3298 mL 1.6491 mL 3.2983 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存

3、条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (8.25 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (8.25 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (8.25 mM)

4、; Clear solution1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 Sardomozide dihydrochloride种 S-腺苷甲硫氨酸脱羧酶 (SAMDC) 抑制剂，其 IC50 值为 5 nM。IC50 & Target IC50: 5 nM (SAMDC) 1体外研究 Sardomozide is a S-adenosylmethionine decarboxylase (SAMDC) inhibitor with an IC50 of 5 nM in cellassay.

5、Following treatment for 48 h with 3 M Sardomozide, intracellular SAMDC activity is reduced to 10%of control 1. When the CHO/664 cells are grown in the presence of Sardomozide and during treatment withDENSPM, vacuole formation is not observed, and these cells are growth-inhibited and contain levels o

6、fDENSPM similar to the parental CHO cells 2.PROTOCOLCell Assay 2 The parent CHO cell line is chronically exposed to increasing levels of Sardomozide for at least eightpassages beginning at 0.1 M. Cell lines are serially exposed to increasing concentrations until a panel ofsublines is obtained resist

7、ant to 1 (CHO/1). 3 (CHO/3), 10 (CHO/10), 30 (CHO/30), and 100 (CHO/100) MSardomozide for comparative studies 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Regenass U, et al. CGP 48664, a new S-adenosylmethionine decarboxylase inhibitor

8、 with broad spectrum antiproliferative and antitumoractivity. Cancer Res. 1994 Jun 15;54(12):3210-7.2. Kramer DL, et al. Lysosomal sequestration of polyamine analogues in Chinese hamster ovary cells resistant to the S-adenosylmethionine decarboxylase inhibitor, CGP-48664. Cancer Res. 1998 Sep 1;58(17):3883-90.McePdfHeightCaution: Product has not been fully validated for medical application