IM-12 - GSK-3 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEIM-12Cat. No.: HY-12292CAS No.: 1129669-05-1分式: CHFNO分量: 377.41作靶点: GSK-3作通路: PI3K/Akt/mTOR; Stem Cell/Wnt储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 54.9 mg/mL (145.47 mM)* means solub

2、le, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.6496 mL 13.2482 mL 26.4964 mL5 mM 0.5299 mL 2.6496 mL 5.2993 mL10 mM 0.2650 mL 1.3248 mL 2.6496 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当

3、天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.62 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE物活性 IM-12是GSK-3 的抑制剂，IC50 值为 53 nM，可增强 Wnt 信号传导。IC50 & Target GSK-353 nM (IC50)

4、体外研究 IM-12 inhibits GSK-3 in ReNcell VM cells, with I50 of 3.8 M. IM-12 (3 M) enhances the -catenin amount,with no further effect at lower or higher concentration. IM-12 (3 M) also attenuates the proliferation ofReNCell VM cells. IM-12 increases TCF-activity of ReNcell VM 1.PROTOCOLKinase Assay 1 Ce

5、lls are lysed in RIPA buffer, supplemented with protease and phosphatase inhibitors and centrifuged for 5min at 15,000 rpm. Immunoprecipitation of GSK-3 is performed with a specific mouse monoclonal anti GSK-3 G8 antibody with 5 g/sample for 2 h at 4C. The bound protein is precipitated with Protein

6、A/G-Plusagarose-beads (10 L beads per sample). GSK-3 kinase activity is measured in a reaction mixturecontaining final concentrations of: 4 mM MOPS pH 7.2; 0.4 mM EDTA; 1 mM EGTA; 2.5 mM -glycerophosphate; 4 mM MgCl2; 40 M BSA; 0.05 mM DTT. 10 g/sample pGS-2 peptide substrate is used1.MCE has not in

7、dependently confirmed the accuracy of these methods. They are for reference only.Cell Assay 1 To measure viable cells, 50-100 L of cell suspension is analyzed using CASY technology with theappropriate program. ReNcell VM cells are seeded at a defined cell number and proliferated for 24 h. Thenthe me

8、dium is changed to proliferation medium with added substances at indicated concentrations. The cellnumber is determined every 24 h. Cells are exposed to the added drugs during the whole experiment,whereas the media is changed every 24 h 1.MCE has not independently confirmed the accuracy of these met

9、hods. They are for reference only.户使本产品发表的科研献 J Cell Physiol. 2019 Jul;234(7):10698-10708.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Schmole AC, et al. Novel indolylmaleimide acts as GSK-3beta inhibitor in human neural progenitor cells. Bioorg Med Chem. 2010 Sep15;18(18):6785-95.McePdfHeightCaution: Product has not been fully validated for medic