KC7F2-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEKC7F2Cat. No.: HY-18777CAS No.: 927822-86-4分式: CHClNOS分量: 570.38作靶点: HIF/HIF Prolyl-Hydroxylase作通路: Metabolic Enzyme/Protease储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 32 mg/mL (56.10

2、mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.7532 mL 8.7661 mL 17.5322 mL5 mM 0.3506 mL 1.7532 mL 3.5064 mL10 mM 0.1753 mL 0.8766 mL 1.7532 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 KC7F2种有效的缺氧诱导因 HIF-1 通道抑制剂，在 LN229-HRE-A

3、P 细胞中 IC50 值为 20 M，可作癌症治疗剂 1。IC50 & Target IC50: 20 M (HIF-1, LN229-HRE-AP cells) 1体外研究KC7F2 (1525 M; 0-72 hours) exhibits a clear dose-response cytotoxicity with an IC50 value of1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEapproximately 1525 M depending on the cell lines, and this effect is mor

4、e severe under hypoxic conditions1.KC7F2 (0-80 M; 6 hours) specifically reduces the protein levels of HIF-1 in a dose-dependent mannerunder hypoxic conditions; strongly decrease in HIF-1 levels at concentrations above 20 M 1.KC7F2 does not affect the rate of HIF-1 protein degradation 1.KC7F2 inhibit

5、s HIF-1 protein synthesis but not its mRNA transcription 1.KC7F2 represses the phosphorylation of eukaryotic initiation factor 4E binding protein 1 (4EBP1) 1.Cell Cytotoxicity Assay 1Cell Line: MCF7 cells, LNZ308 cells, A549 cells, U251MG cells, LN229 cellsConcentration: 1525 MIncubation Time: 0-72

6、hoursResult: With cytotoxicity more pronounced in tumor cell lines as compared to normal cells.Cell Viability Assay 1Cell Line: LN229 cellsConcentration: 6 hoursIncubation Time: 0 M, 5 M,7.5 M,10 M,15 M,20 M,30 M,40 M,60 M,80 MResult: Decreases HIF-1 protein levels in a dose-dependent manner.户使本产品发表

7、的科研献 BMC Anesthesiol. 2019 Jul 9;19(1):127See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Narita T, et al. Identification of a novel small molecule HIF-1alpha translation inhibitor. Clin Cancer Res. 2009 Oct 1;15(19):6128-6136.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use