美国FDA细菌学分析手册第八版沙门氏菌

1、矮BAM: Sa袄lmonell疤aNOTICE:八If you 跋are loo摆king fo笆r BAM C肮hapter 碍5: 拜Salmone挨lla办 (Decem摆ber 200般7 Editi摆on) tha扳t is in跋corpora跋ted by 半referen耙ce in 2安1 CFR P碍arts 16昂 and 11稗8: HYPERLINK /Food/FoodSafety/Product-SpecificInformation/EggSafety/EggSafetyActionPlan/ucm170615.htm 疤Federal柏 Regist佰er

2、Fina白l Rule扳1扒 (July 邦9, 2009柏, 74 FR瓣 33030)把: Preve翱ntion o哀f Salmo肮nella E岸nteriti安dis in 癌Shell E俺ggs Dur鞍ing Pro蔼duction背, Stora绊ge, and昂 Transp坝ortatio绊n, plea埃se use 氨thesev蔼ersions扒 of the罢 BAM HYPERLINK /downloads/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/UCM

3、244774.pdf 叭Salmone爱lla翱 Chapte爱r案2班 (PDF, 班189 Kb)扳and HYPERLINK /downloads/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/UCM244777.pdf 败Appendi叭x 1: Ra岸pid Met拜hods fo阿r Detec胺ting Fo敖odborne搬 Pathog拜ens埃3百 (PDF, 哀195 Kb)靶.稗The mos瓣t recen俺tEditi凹on of B翱AM Chap坝ter 5:

4、吧Salmone办lla拔 is ava巴ilable 啊below t皑his not背ice.办Novembe爸r 2011 隘Version版Bacteri八ologica敖l Analy捌tical M爸anual坝Chapter碍 5靶Salmone绊lla阿Authors爱: Walla吧ce H. A盎ndrews 氨and HYPERLINK mailto:Thomas.Hammack 霸Thomas 绊Hammack俺Revisio斑n Histo阿ry蔼:颁Novembe办r 2011 巴- Addit伴ion to 凹Section鞍 C: Pre皑paratio颁n o

5、f fo隘ods for斑 isolat把ion of 熬Salmone澳lla办: Leafy唉 green 邦vegetab哎les and罢 herbs.碍 班Februar懊y 2011 袄Remo败ved lin搬k to Ap氨pendix 隘1: Rapi板d Metho肮ds for 肮Detecti案ng Food靶borne P爸athogen芭s (now 摆archive吧d). 稗Decembe皑r 2007 瓣 Mamey邦 pulp m埃ethod a摆dded, a巴nd Sect隘ion D r八evised.案 叭June 20胺06 Eg爱gs meth爱

6、od revi芭sed for按 shell 爱eggs an矮d liqui癌d whole班 eggs. 柏April 2爱003 F鞍rog leg叭s metho扳d, Lact拔ic case笆in, Ren败net cas捌ein, So蔼dium ca翱seinate熬 and Ra熬bbit ca挨rcass m袄ethods 罢revised芭, top e懊ars and绊 other 袄dog che鞍w toys 拔added. 霸Removed拜 sectio爸n A.25,澳 Mechan背ical sh白aker. 盎October盎 25, 20蔼01 Ex八t

7、ension斑 of the芭 applic哀ability斑 of the叭 orange哀 juice 案method 癌in sect瓣ion C.1癌9 to ap拔ple jui霸ce and 佰apple c半ider. 版1999-DE捌C, 2000巴-MAR, a爱nd 2000耙-AUG Fi奥nal rev碍ision o败n 2000-班NOV-14 挨(see th稗e Intro哀duction板 for a 蔼summary佰 of cha澳nges).阿To obta挨in a co靶py of a版 prior 胺version佰 not cu案rrently

8、摆 posted敖, pleas板e conta昂ct HYPERLINK mailto:Thomas.Hammack 霸Thomas 暗Hammack澳Chapter背 Conten绊ts HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Intro#Intro l Intro#Intro 败Introdu爱ction扒 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriologic

9、alAnalyticalManualBAM/ucm070149.htm#Equip#Equip l Equip#Equip 板Equipme罢nt and 唉Materia拔ls奥 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Media#Media l Media#Media 半Media a隘nd Reag案ents笆 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriolog

10、icalAnalyticalManualBAM/ucm070149.htm#Prep#Prep l Prep#Prep 班Prepara版tion of笆 foods 拔for iso靶lation 安of 昂Salmone芭lla版 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Isol#Isol l Isol#Isol 翱Isolati澳on of 百Salmone邦lla捌 HYPERLINK /Food/ScienceResearch/

11、LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Id#Id l Id#Id 斑Identif斑ication啊 of 隘Salmone氨lla拔 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 绊Referen碍ces啊Introdu艾ction瓣Several拜 change背s are b俺eing in挨trod

12、uce敖d in th吧is edit癌ion of 凹BAM (8颁th版 Editio八n). The柏 first 扮change 澳involve啊s the e翱xpanded凹 use of百 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063568.htm 昂Rappapo凹rt-Vass扳iliadis矮 (RV) m敖edium版4板 for fo佰ods wit半h both 懊high an隘d low l瓣evels o八f compe暗t

13、itive 阿microfl班ora. In暗 the pr隘evious 柏edition般, RV me奥dium wa俺s recom巴mended 笆only fo啊r the a袄nalysis昂 of shr跋imp. Ba凹sed on 霸the com碍pletion扒 of AOA把C preco吧llabora隘tive ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 靶5, 6

14、佰) and c埃ollabor爱ative ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 半7, 8版) studi斑es, RV 败medium 爸is now 版being r颁ecommen岸ded for板 the an案alysis 俺of high皑 microb爱ial and版 low mi绊crobial俺 load f吧oods. R半V mediu拔m repla碍ces

15、sel八enite c搬ystine 败(SC) br八oth for白 the an癌alysis 唉of all 斑foods, 搬except 矮guar gu昂m. In a挨ddition敖, RV me凹dium re拔places 班lauryl 霸tryptos败e broth吧 for us皑e with 爱dry act瓣ive yea艾st. HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063686.htm 凹Tetrath版ionate 盎

16、(TT)岸5疤 broth 傲continu昂es to b昂e used 伴as the 稗second 翱selecti吧ve enri吧chment 白broth. 澳However叭, TT br鞍oth is 邦to be i埃ncubate鞍d at 啊43C霸 for th蔼e analy懊sis of 懊high mi懊crobial熬 load f疤oods an扳d at 板35C凹 for th疤e analy哎sis of 盎low mic澳robial 背load fo隘ods, in肮cluding挨 guar g搬um.罢The sec败ond cha袄nge i

17、nv稗olves t拌he opti敖on of r熬efriger坝ating i扳ncubate熬d preen懊richmen盎ts and 般selecti哀ve enri罢chments摆 of low肮-moistu伴re food搬s for u爱p to 72肮 h. Wit叭h this 百option,伴 sample安 analys白es can 版be init埃iated a澳s late 百as Wedn靶esday o柏r Thurs百day wit案hout we白ekend w绊ork bei伴ng invo拔lved.稗The thi叭rd chan肮ge

18、invo半lves re拌ducing 癌the per蔼iod of 皑incubat把ion of 叭the HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064484.htm 懊lysine 半iron ag胺ar (LIA叭)捌6疤 slants办. In th袄e forme版r editi半on (BAM柏-7), HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnaly

19、ticalManualBAM/ucm063699.htm 百triple 柏sugar i哀ron aga澳r (TSI)板7芭 and LI隘A slant岸s were 皑incubat唉ed at 芭35C拌 for 24耙 2 h 哎and 48 澳 2 h, 氨respect叭ively. 版Unpubli傲shed da疤ta have百 demons胺trated 百that th扳e 48 h 澳reading爱 of LIA癌 slants扳 is wit坝hout di昂agnosti耙c value唉. Of 19版3 LIA s安lants e扳xamined办, al

20、l g吧ave def懊initive斑 result吧s withi罢n 24 搬2 h of 扮incubat安ion. No岸 signif翱icant c芭hanges 靶altered案 the fi鞍nal tes岸t resul阿t when 办the sla扳nts wer瓣e incub疤ated an霸 additi靶onal 24扮 h. Thu罢s, both邦 the TS般I and L哀IA slan背ts are 案now inc佰ubated 胺for 24 皑 2 h.蔼The fou蔼rth cha疤nge inv稗olves t疤he proc皑edur

21、e f澳or surf哀ace dis盎infecti阿on of s敖hell eg白gs. In 艾the pre芭vious e扳dition 霸(BAM-7)按, egg s耙hells w拌ere sur耙face-di搬sinfect绊ed by s扒oaking 凹in 0.1%胺 mercur拔ic chlo隘ride so佰lution 邦for 1 h拔 follow阿ed by s暗oaking 安in 70% 笆ethanol霸 for 30翱 min. M傲ercuric拔 chlori吧de is c哀lassifi挨ed as a扒 hazard白ous was半

22、te, and邦 is exp板ensive 扳to disp鞍ose of 摆accordi般ng to E岸nvironm绊ental P癌rotecti半on Agen鞍cy guid稗elines.吧 In thi绊s editi敖on (BAM唉-8) egg隘 shells扒 are no巴w surfa埃ce-disi绊nfected盎 by soa疤king fo邦r at le霸ast 10 疤sec in 扒a 3:1 s笆olution靶 consis阿ting of巴 3 part阿s of 70佰% alcoh背ol (eth肮yl or i坝sopropy坝l) t

23、o 1敖 part o安f iodin疤e/potas靶sium io邦dide so靶lution.唉The fif拌th chan艾ge invo般lves th熬e sampl斑e prepa叭ration 阿of eggs哀. Egg c胺ontents拌 (yolk 按and alb背umen) a拌re thor八oughly 熬mixed b鞍efore a巴nalysis拜. After盎 mixing版 the eg爱g conte奥nts, 罢25 g摆 (ml) a澳re adde坝d to 22跋5 ml tr暗ypticas啊e (tryp背tic) so颁y bro

24、th柏 supple办mented 般with fe百rrous s爸ulfate.捌A metho败d for t摆he anal绊ysis of稗 guar g氨um has 癌been in吧cluded.笆 When g扮uar gum哀 is pre般enriche哀d at a 按1:9 sam瓣ple/bro柏th rati办o, a hi芭ghly vi办scous, 半nonpipe爱ttable 肮mixture瓣 result巴s. Addi稗tion of澳 the en爸zyme ce瓣llulase阿 to the傲 preenr靶ichment巴 medium摆,

25、 howev癌er, res扮ults in澳 a read扳ily pip斑ettable疤 mixtur爸e.般A metho皑d for o百range j蔼uice (p佰asteuri扳zed and伴 unpast班eurized啊) has b挨een inc盎luded d碍ue to r岸ecent o扒range j坝uice-re奥lated o隘utbreak懊s.办The dir俺ections爸 for pi邦cking c邦olonies般 from t佰he sele鞍ctive p佰lating 鞍agars h挨ave bee邦n made 佰more ex

26、败plicit 柏to refl翱ect the岸 intent隘 of the敖 method背. In th背e absen佰ce of t邦ypical 鞍or susp翱ect col版onies o盎n the s败electiv跋e plati伴ng agar半s, it i案s recom傲mended 扳that at安ypical 靶colonie矮s be pi白cked to扒 TSI an邦d LIA s稗lants. 柏This re邦commend岸ation i般s based埃 on the拌 fact t拜hat up 懊to 4% o芭f all 扳Salm

27、one八lla拜 cultur佰es isol般ated by隘 FDA an扳alysts 班from ce罢rtain f芭oods, e扳special吧ly seaf扮oods, d百uring t捌he past版 severa阿l years坝 have b笆een aty矮pical.唉Finally拌, since爸 the pu般blicati颁on of B蔼AM-7, a翱 6-way 矮compari版son was俺 conduc唉ted of 百the rel百ative e蔼ffectiv百eness o芭f the t疤hree se蔼lective按 plat

28、in矮g agars半 recomm霸ended i捌n the B摆AM ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063348.htm 般bismuth氨 sulfit扮e伴8扒, HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064342.htm 昂Hektoen瓣 enteri疤c爸9俺, and HYPERLINK /F

29、ood/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062986.htm 办xylose 扒lysine 叭desoxyc拌holate 袄agars昂10百) and t昂hree re阿lativel吧y new a敖gars (E靶F-18, x扮ylose l罢ysine T背ergitol稗 4, and佰 Rambac笆h agars傲). Our 扮results扒 (9) in阿dicated袄 no adv爱antage 佰in repl暗acing a懊ny of t皑he

30、BAM-芭recomme拌nded ag败ars wit奥h one o肮r more 把of the 稗newer a柏gars. T按hus, th案e combi坝nation 霸of sele埃ctive p奥lating 爸agars r板ecommen拌ded in 跋BAM-7 r芭emains 昂unchang啊ed.奥Equipme跋nt and 颁Materia安ls暗 唉Blender拔 and st翱erile b敖lender 挨jars (耙see败 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacterio

31、logicalAnalyticalManualBAM/ucm063335.htm 白Chapter稗 1唉11案) 坝Sterile叭, 巴16 oz挨 (500 m班l) wide败-mouth,笆 screw-鞍cap jar懊s, ster办ile 500埃 ml Erl盎enmeyer坝 flasks碍, steri矮le 250 笆ml beak袄ers, st拌erile g爸lass or跋 paper 巴funnels哎 of app背ropriat凹e size,稗 and, o八ptional凹ly, con懊tainers白 of app扒ropriat办e capac吧

32、ity to 般accommo稗date co挨mposite凹d sampl胺es 邦Sterile拌, bent 敖glass o暗r plast佰ic spre俺ader ro矮ds 爸Balance罢, with 柏weights拜; 捌2000 g拔 capaci败ty, sen盎sitivit啊y of 八0.1 g半 伴Balance叭, with 哎weights啊; 扒120 g叭 capaci矮ty, sen版sitivit氨y of 5 拜mg 叭Incubat稗or, 35 摆 摆2 C靶 皑Refrige氨rated i碍ncubato耙r or la埃borator

33、霸y refri半gerator敖, 4 懊2C奥 百Water b颁ath, 49鞍 白1C盎 暗Water b暗ath, ci摆rculati背ng, the拜rmostat胺ically-班control熬led, 43艾 挨0.2C败 傲Water b挨ath, ci碍rculati暗ng, the疤rmostat岸ically-搬control凹led,42 哎 傲0.2C肮 稗Sterile鞍 spoons搬 or oth班er appr碍opriate挨 instru罢ments f哎or tran矮sferrin坝g food 阿samples拜 耙Sterile懊 cultu

34、r霸e dishe氨s, 15 x邦 敖100 mm跋, glass疤 or pla爱stic 凹Sterile爸 pipets柏, 1 ml,瓣 with 0矮.01 ml 唉graduat胺ions; 5板 and 10皑 ml, wi氨th 0.1 百ml grad袄uations爸 稗Inocula靶ting ne叭edle an挨d inocu拔lating 伴loop (a俺bout 斑3 mm扮 id or 耙10 蔼5l澳), nich芭rome, p拔latinum办-iridiu皑m, chro俺mel wir罢e, or s摆terile 蔼plastic岸 疤Steri

35、le邦 test o肮r cultu耙re tube芭s, 16 x胺 搬150 mm癌 and 20叭 x 版150 mm罢; serol皑ogical 拜tubes, 斑10 x 办75 mm瓣 or 13 伴x 耙100 mm皑 半Test or安 cultur哎e tube 扒racks 跋Vortex 靶mixer 唉Sterile鞍 shears版, large巴 scisso碍rs, sca挨lpel, a唉nd forc氨eps 肮Lamp (f胺or obse佰rving s搬erologi叭cal rea隘ctions)巴 巴Fisher 班or Buns捌en burn鞍

36、er 爸pH test板 paper 摆(pH ran癌ge 6-8)半 with m唉aximum 岸graduat胺ions of稗 0.4 pH吧 units 唉per col笆or chan耙ge 盎pH mete肮r 哎邦Plastic翱 bags, 奥28 x 拔37 cm案, steri按le, wit白h resea巴lable t搬ape. (I办tems 23皑-24 are暗 needed埃 in the案 analys背is of f癌rog leg半s and r百abbit c摆arcasse佰s.) 八Plastic傲 beaker叭s, 4 li扳ter, au

37、肮toclava般ble, fo绊r holdi暗ng plas搬tic bag胺 during板 shakin岸g and i疤ncubati艾on. 搬Sponges奥, non-b班acteric扒idal (N昂asco ca拔t # B01白299WA),笆 or equ捌ivalent拔. 艾Swabs, 搬non-bac白tericid熬al, cot百ton-tip芭ped.澳 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm055778.htm 隘M

38、edia靶12岸 and HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm055791.htm 昂Reagent胺s澳13盎For pre拔paratio霸n of me搬dia and摆 reagen哎ts, ref搬er to M爸ethods 碍967.25碍-967.28昂 in蔼 稗Officia奥l Metho唉ds of A埃nalysis隘 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bact

39、eriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 搬1唉).巴Lactose懊 broth 拔( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064414.htm 傲M74澳14蔼) 袄Nonfat 瓣dry mil矮k (reco爱nstitut八ed) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriolog

40、icalAnalyticalManualBAM/ucm064144.htm 颁M111白15艾) 坝Selenit翱e cysti奥ne (SC)案 broth 翱( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063597.htm 版M134芭16埃) 皑Tetrath背ionate 阿(TT) br安oth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical

41、ManualBAM/ucm063686.htm 疤M145矮17柏) 奥Rappapo颁rt-Vass跋iliadis斑 (RV) m胺edium ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063568.htm 安M132埃18碍). NOTE跋: RV me瓣dium mu摆st be m伴ade fro捌m its i败ndividu按al ingr罢edients背. Comme哎rcial f岸ormulat柏ions ar罢e not a捌ccep

42、tab摆le. 疤Xylose 氨lysine 阿desoxyc岸holate 败(XLD) a扒gar ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062986.htm 半M179艾19敖) 罢Hektoen奥 enteri叭c (HE) 哎agar ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064342.htm 矮M61

43、靶20碍) 叭Bismuth搬 sulfit坝e (BS) 耙agar ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063348.htm 斑M19埃21盎) 氨Triple 佰sugar i啊ron aga笆r (TSI)邦 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063699.htm 癌M149百22罢) 按Trypto

44、n巴e (tryp盎tophane巴) broth案 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063867.htm 哎M164版23叭) 笆Tryptic斑ase (tr蔼yptic) 拜soy bro瓣th ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063805.htm 八M154安24翱) 霸Tryptic疤ase

45、soy稗 broth 安with fe班rrous s败ulfate 半( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063816.htm 巴M186靶25霸) 半Tryptic班ase soy氨-trypto扒se brot案h ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063862.htm 坝M160稗26岸) 板MR-V

46、P b鞍roth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064068.htm 背M104暗27癌) 扮Simmons癌 citrat傲e agar 傲( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063639.htm 稗M138拜28盎) 盎Urea br跋oth ( HYPERLINK /Food/ScienceRese

47、arch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062935.htm 伴M171疤29摆) 霸Urea br柏oth (ra澳pid) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062967.htm 班M172澳30瓣) 把Malonat斑e broth哎 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriologic

48、alAnalyticalManualBAM/ucm064500.htm 拌M92败31胺) 俺Lysine 佰iron ag澳ar (LIA笆) (Edwa八rds and阿 搬Fife蔼) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064484.htm 盎M89摆32皑) 伴Lysine 霸decarbo爸xylase 班broth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriolo

49、gicalAnalyticalManualBAM/ucm064474.htm 翱M87)绊33爱 扒Motilit啊y test 拜medium 巴(semiso办lid) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064059.htm 挨M103颁34摆) 爱Potassi邦um cyan岸ide (KC办N) brot懊h ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriologica

50、lAnalyticalManualBAM/ucm063514.htm 百M126靶35傲) 哎Phenol 熬red car笆bohydra伴te brot稗h ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063494.htm 拜M121爸36昂) 颁Purple 肮carbohy扒drate b耙roth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical

51、ManualBAM/ucm063529.htm 安M130隘37版) 巴MacConk半ey agar昂 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064496.htm 背M91癌38靶) 靶Nutrien盎t broth癌 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064169.htm 巴M114爱39矮) 唉Brain

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54、LINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062927.htm 氨M188吧44隘) 拔Univers澳al pree蔼nrichme背nt brot俺h (with柏out fer摆ric amm按onium c白itrate)懊 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062933.htm 暗M188a袄45板) 鞍Buffere

55、吧d pepto盎ne wate摆r ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063366.htm 阿M192稗46哎) 隘Dey-Eng艾ley bro阿th ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064244.htm 扳M193白47柏) 岸Potassi奥um sulf鞍ite pow柏der, an把hydr

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