HLCL-61-hydrochloride - Histone Methyltransferase 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHLCL-61 hydrochlorideCat. No.: HY-100025ACAS No.: 1158279-20-9分式: CHClNO分量: 380.91作靶点: Histone Methyltransferase作通路: Epigenetics储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 50 mg/mL (131

2、.26 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.6253 mL 13.1265 mL 26.2529 mL5 mM 0.5251 mL 2.6253 mL 5.2506 mL10 mM 0.2625 mL 1.3126 mL 2.6253 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；

3、澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.56 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (6.56 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/

4、mL (6.56 mM); Clear solution1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 HLCL-61 hydrochloride流的分蛋精氨酸甲基转移酶 5 (PRMT5) 抑制剂。IC50 & Target Protein arginine methyltransferase 5 1体外研究 HLCL-61 hydrochloride (1-100 M; 24-72 hours) reduces cells growth at dose-dependent manner with

5、 IC50sof 14.12, 16.74, 6.3, 8.72 M for MV4-11 cells, THP-1 cells, FLT3-WT blast, and FLT3-ITD blast, respectively1.HLCL-61 hydrochloride shows effective inhibition of symmetric arginine dimethylation (me2) of histones H3and H4 in AML samples, starting at 12h post-treatment and persisting after 48h 1

6、.Cell Viability Assay 1Cell Line: MV4-11 cells, THP-1 cells, FLT3-WT blast (primary blasts from patients), FLT3-ITD blast(primary blasts from patients)Concentration: 1, 5, 10, 25, 50, 100 MIncubation Time: 24, 48, 72 hoursResult: Dose-dependent reduction in cell viability with IC50s of 14.12, 16.74,

7、 6.3, 8.72 M forMV4-11 cells, THP-1 cells, FLT3-WT blast, and FLT3-ITD blast, respectively.户使本产品发表的科研献 Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):2961-2966.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Tarighat SS, et al. The dual epigenetic role of PRMT5 in acute myeloid leukemia: gene activation and repression via histone argininemethylation. Leukemia. 2016 Apr;30(4):789-99.McePdfHeightCaution: Product has not been fully validated for medical applications.For resea