Forchlorfenuron-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEForchlorfenuronCat. No.: HY-B1841CAS No.: 68157-60-8分式: CHClNO分量: 247.68作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 29 mg/mL (117.09 mM)* means soluble, but satura

2、tion unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 4.0375 mL 20.1873 mL 40.3747 mL5 mM 0.8075 mL 4.0375 mL 8.0749 mL10 mM 0.4037 mL 2.0187 mL 4.0375 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Forchlorfenuron植物长调节剂和细胞分裂素; 可于增加果的果实，如猕猴桃和葡萄。体外研究Forchlorfenuron is

3、a plant growth regulator, applied extensively to increase kiwifruit size and weight.Cytotoxicity of forchlorfenuron and its metabolites are tested through Sulforhodamine B assays against CHOcells. Forchlorfenuron exhibits significant cytotoxicity against CHO cells with an IC50 of 12.122.14 M 1.The h

4、alf-lives offorchlorfenuron were 15.8-23.0 days, the final residues of forchlorfenuron in pulp were all1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE0.002 mg/kg, and most of the residues were concentrated in the peel. The risk assessment revealed that nosignificant potential health risk would be

5、induced by forchlorfenuron in citrus fruits. Therefore, it could be safeto apply forchlorfenuron in citrus fruits 2.PROTOCOLCell Assay 1 CHO cells are cultured in high-glucose DMEM medium. Forchlorfenuron and its kiwifruit metabolites aredissolved in DMSO and diluted in culture media. Cells are seed

6、ed in a 96-well flat microtiter plate for 24 hand are then incubated for an additional 48 h with various concentrations of the compounds. Next, cells arefixed with 10% trichloroacetic acid and incubated for 60 min at 4 C. The supernatant is discarded, then theplates are washed. Using 0.4% SRB soluti

7、on dissolved in 1% acetic acid, the cell layer is strained, then theplates are incubated for 20 min at room temperature. The stained cells is solubilized in 10 mM un-bufferedTris base and optical density (OD) is measured at 560 nm 1.MCE has not independently confirmed the accuracy of these methods.

8、They are for reference only.REFERENCES1. Zhang Z, et al. Identification, synthesis, and safety assessment of forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea) and itsmetabolites in kiwifruits. J Agric Food Chem. 2015 Mar 25;63(11):3059-66.2. Chen W, et al. Dissipation and residue of forchlorfenuron in citrus fruits. Bull Environ Contam Toxicol. 2013 Jun;90(6):756-60.McePdfHeightCaution: Product has not been fully validated for medical applicat