GSK2200150A - Bacterial 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEGSK2200150ACat. No.: HY-112091CAS No.: 1443138-53-1分式: CHNOS分量: 357.47作靶点: Bacterial作通路: Anti-infection储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 83.33 mg/mL (233.11 mM)* means soluble

2、, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.7974 mL 13.9872 mL 27.9744 mL5 mM 0.5595 mL 2.7974 mL 5.5949 mL10 mM 0.2797 mL 1.3987 mL 2.7974 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 GSK2200150A通过通量表型筛选确定的种抗结核剂。对 H37Rv 的 MIC是0.38 M。IC50 & T

3、arget MIC: 0.38 M (M.tuberculosis strain H37Rv) 1体外研究GSK2200150A is a novel antimycobacterial agent against Mycobacterium tuberculosis.The activities ofGSK2200150A containing the spirocycle core are determined against the virulent M.tuberculosis strain1/2 Master of Small Molecules 您边的抑制剂师www.MedChem

4、E(H37Rv) with MIC of 0.38 M 1.PROTOCOLCell Assay GSK2200150A is tested for activity at either single concentration (100 M) or serially diluted in 10 L ofpurified H2O in triplicate in 96 well microtiter plates. M. tuberculosis H37Rv is grown in complete Middlebrook7H9 media containing albumin, dextro

5、se and catalase (ADC), 20% Tween 80 and 50% glycerol. A bacterialsuspension (90 L) at OD600 nm of 0.001 is added to the wells and incubated for 7 days. Resazurin (10 L;0.05%(w/v) is then added, incubated for 24 h at 37C, and fluorescence measured at 590 nm using aFLUOstar Omega microplate reader. Af

6、ter subtraction of background fluorescence from all wells, thepercentage mycobacterial survival is determined by comparing the fluorescence of wells containingcompounds compared to control wells not treated with compound 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Badiola KA, et al. Efficient Synthesis and Anti-Tubercular Activity of a Series of Spirocycles: An Exercise in OpenScience. PLoS One.2014 Dec 10;9(12):e111782.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use on