过氧化氢检测研究

1、过氧化氢检测研究页 过氧化氢检测研究检索报告过氧化氢检测研究小组成员郝同港房静远刘明阳史迎利完成时间：2016/4/1目录 TOC o 1-5 h z .课题分析3.文献范围和检索工具4.检索词和检索式的析取4.检索过程及结果 5维普期刊5万方数据库6 HYPERLINK l bookmark9 o Current Document Science Direct8 HYPERLINK l bookmark13 o Current Document Web of Science10中国知识产权局12.检索体会13.课题分析随着人类文明的进步与发展，越来越多的国家与地区意识到 化学污染的严重性，绿

2、色化学成为关注的焦点。在化学工业 中，污染物主要为残余的氧化剂，这些氧化剂会对自然环境 及生命体造成严重危害。而过氧化氢作为一种无污染的氧化 剂，自然成为人们追捧的对象。并且过氧化氢在化学的生产 中也充当着重要作用。具氧化分解产物为水，对大多数工业 生产体系不带来任何杂质，并且在自然界中过氧化氢会缓慢 分解为氧气和水，对自然生命体并无致命危害。在化学工业 生产中，过氧化氢可以胜任多种体系环境，不论酸性碱性环 境都有较高氧化能力，并且在特定环境下可以充当还原剂， 这种高性能的试剂是非常罕见的。所以在化学工业中，检测 过氧化氢是必不可少的项目。并且过氧化氢生产成本较高， 在大型工业中，检测过氧化氢

3、的含量有助于把握过氧化氢的 用量，避免过量使用过氧化氢从而达到降低成本。本课题旨 在利用所学文献检索知识，检索在各种化学环境体系下检测 过氧化氢的方法，经过阅读与整理文献，了解此类研究的最 新研究进展，总结其中难点以及解决难点的方法。.文献范围和检索工具中文数据库维普期刊万方数据库外文数据库Web of ScienceScience Direct专利国家知识产权局其中包括了全文型数据库，也有文摘型数据库和专利数据库, 相信通过对这些数据库的搜索，收集的文献涉及面广阔，研 究水平也比较高，可以满足课题的要求。.检索词和检索式的析取课题中关键检索词为“过氧化氢”，而过氧化氢又有名 称双氧水，因此应

4、该将二者用“或”关系连接。在课题中，“检测”可能在一些文献中为“鉴定” 、“分 析”等，故应为“或”关系连接。课题中“研究” 一词过于宽泛，故在检索式中应不予体 现，否则影响查全率。在检索中可以加以一些检索词进行限制或者精炼以提高查准率，不过为了保证查全率， 可以采取先宽泛检索再进行二次检索或二次精炼的方法，同时保证查全率和查准率。确定过氧化氢，双氧水，鉴定， 分析，检测为检索关键 字。对于英文检索词，注意截词符的使用，因为英语中一个 词义的表示有多种形式。使用截词符可以最大限度地提 高检索的查全率。核心检索词： perhydrol , hydrogen peroxide, detection

5、, identify 。通用检索式：(过氧化氢+双氧水)* (检测+鉴定 +分析)(perhydrol+ hydrogen peroxide )* (detection + identify )。.检索过程及结果维普期刊先通过维普数据库初步了解过氧化氢的检测方法和可以用来检测的物品。M=（双氧水+过氧化氢+ H2Q） *检测时间限制为20072016年，得到200条搜索结果，选择相关性的4篇文献如下：. 丁梦玲 丁赔赔张天宇赵真田 孙乐乐 冒爱琴单分散纳米银胶体的快速制备及其在 H2O2检测中的应用出处：过程工程学报 CAS CSCD 2015年第5期。.彭池方段小慧谢正军李炎基于纳米金生成的

6、过氧化氢快速检测方法出处：食品与生物技术学报 CAS CSCD 2015年第10期.李娜娜黄嘉荣詹求强基于纳米金表面等离子体的高灵敏过氧化氢光学检测方法在线阅读 出处：华南师范大学学报：自然科学版CAS 2015年第4期.万姝岐翁前锋纳米氧化铜/石墨烯修饰电极的制备及对H2Q的检测在线阅读下载全文出处：广州化工 CAS 2015年第10期万方数据库通过进一步研究，深入了解双氧水检测中的一些常见方法，和常见的一些检测技术，了解最新的双氧水研究动态，现状，发展方向。检索式：（双氧水 + 过氧化氢 +h2o2+H2O2+Hydrogen peroxide）* 检测对于在这个数据库中的检索整理得到下面

7、2个方面的检测研究：1）食品方面：1张勇.食品中外源性H202（双氧水）的现场定性检测研究J.企业技术开发（学术版）,2011,30（2）:30-31.机构：驻马店市质量技术监督检验测试中心，河南，驻马店，463000摘要：文章主要介绍食品中外源性H2O2砥氧水)的现场定性检测研究.3庞军，宋合兴，刘博等.牛奶中H2O2残留快速检测技术J.中国奶 牛,2013,(19):49-53.DOI:10.3969/j.issn.1004-4264.2013.19.014.机构：河北科技大学生物科学与工程学院，石家庄，050018摘要：目前，过氧化氢(H2O2)作为廉价有效的氧化剂、漂白剂以及防腐剂，已

8、被一些食品生产企业超量或非法使用，对消费者的健康造成危害.因此，建立快速、高效的过氧化氢残留检测 方法有着重要的意义.本文主要介绍了近几年牛奶及其他食品中过氧化氢残留快速检测技术 及其应用状况.5程楠，董凯，何景等.食品中过氧化氢残留快速检测试纸的研制与应用J.农业生物技术学报,2013,21(12):1403-1412.DOI:10.3969/j.issn.1674-7968.2013.12.001.机构：中国农业大学食品科学与营养工程学院，北京,100083;中国农业大学食品科学与营养工程学院，北京100083;北京福德安科技有限公司，北京100083;农业部转基因生物使用安全 检验监督测

9、试中心，北京,100083;北京福德安科技有限公司，北京,100083;中国农业大学食品科学与营养工程学院，北京100083;农业部转基因生物使用安全检验监督测试中心，北京100083摘要：食品中过氧化氢残留的现象较为严重，为开发一种操作简便、反应迅速、快捷高效的检测方法，本研究建立检测食品过氧化氢残留的试纸快速检测法，并通过测定25组样品中过氧化氢的残留量验证其应用性.现有方法响应时间长、生产周期长、稳定性差，本研究以3 mm层析滤纸为载体，使试纸能够在3s内对过氧化氢残留进行迅速的半定量检测；采用正交实验详细优化制作工艺，极大缩短了试纸的生产周期；在显色液中添加了酶保护剂，使试纸具有了较好

10、的稳定性和保存期；对25组样品进行过氧化氢残留量测定，结果表明，试纸法与国标法结果吻合性高，更为快速、方便、灵敏，而且操作简单、价格低廉，适用于现场食品安全监督 检验，对食品中过氧化氢残留的快速检测具有重要意义2)医学方面：口 董冉冉，张燕，时克林等.毛细管电泳电化学法检测过氧化氢J.化学分析计 量,2011,20(6):26-29.DOI:10.3969/j.issn.1008-6145.2011.06.007.机构：山东师范大学化学化工与材料科学学院，济南,250014;淄博六中，淄博,252300摘要：以金微盘电极和离子液体修饰单壁碳纳米管糊微盘电极分别作为毛细管电泳电化学检 测器，试验

11、了两种电极对过氧化氢的响应情况，将金微盘电极与毛细管电泳联用，对过氧化氢进行了定性和定量检测.探讨了分离电压、缓冲溶液pH值和工作电位等条件对H2O2检测的影响.实验结果表明，峰电流与 H2O2浓度在1.0 X 10-61.0 X 10-5mol/L 和3.0 X 1041.0 X 10-3 mol/L 呈良好的线性，线性回归方程分别为y(nA)=0.04178+0.015 5x(科mol/L),y(A)=-3.3045 X 10-4+0.024 29x (mmol/L),相关系数分别为 0.9975 和 0.9990,当信噪比S/N=3时，浓度检出限为5X l0-7mol/L.实验中发现，样

12、品中的多巴胺、抗坏血酸等对H2O2的测定无干扰.8亓玲.用于检测细胞内过氧化氢的荧光探针J.黑龙江科技信 息,2007,(9):27.DOI:10.3969/j.issn.1673-1328.2007.09.029.机构：山东师范大学化学化工与材料学院，山东，济南,250014摘要：介绍了三种用于检测细胞内过氧化氢的荧光探针，并阐明了荧光探针检测过氧化氢反应的机理.9公晓聪.基于微流控芯片细胞中过氧化氢的检测D.山东师范大学,2009.机构：山东师范大学摘要：细胞的代谢过程中不断产生各种活性氧自由基（ROS）,例如超氧阴离子自由基 （O2-）、羟自由基（ OH卜脂自由基（ROO）、过氧化氢（H

13、2O2）等。它们一方面能够介导生命活动的 众多生理过程，另一方面也能够对组织细胞的化学结构发生破坏性修饰，损伤正常组织细胞的形态和功能。研究表明，细胞中产生的过氧化氢，在一般情况下常被过氧化氢酶和谷胱甘 肽过氧化物酶清除，如果得不到及时清除，它可透过细胞膜与膜外的Fe2+或Cu玲金属离子结合生成H0-,从而导致膜脂过氧化、碱基突变、DNA链的断裂和蛋白质的损伤。因此对过氧化氢进行高灵敏度、高选择性的分析与检测对于研究过氧化氢如何参与各种生命活动是极 为必要的。由于细胞尺度小、细胞内过氧化氢含量低、产生与转化速度快，使得目前一些传统的观察与分析方法多是将各种活性氧作为一个整体，获得的结果多是基于

14、静态、宏观的观察和整体平均而推导出来的。它们普遍存在取样体积大、质量检测限高、分析信号的获得时间长、仪器价格昂贵等方面的不足。因此，在细胞层面上定性及定量测定过氧化氢的含量对当今自由基生物学研究具有重要的学术意义和应用价值。微流控芯片分析（microfluidic chip analysis）具有快速、低耗，并且能将多种功能单元集成于一个芯片等 特点，它向实现维持一个闭合系统迈进了一大步。由于它可以实现完全的自动化，减少污染，降低干扰和人为误差，因而成为近年来生物、化学、医学等领域研究的重要平台之一。本论文以微流控芯片电泳激光诱导荧光分析系统为技术依托，以实验室自行合成的荧光探针为基础，对细胞

15、中的过氧化氢（H2O2）的检测进行了研究。论文共分三个部分。在第一章中，简要阐述了微流控芯片在细胞分析领域的研究的进展。主要对微流控芯片在细胞培养、细胞操纵、细胞溶膜、细胞组分的检测等方面的应用进行了简单的介绍及综述。在第二章中，选择实验室内部合成的荧光探针二（对甲苯磺酰基）-二氯荧光素作为荧光标记试剂，以微流控芯片电泳-激光诱导荧光法作为分析平台，建立了一种未见报道的过氧化氢的检测方法， 并且成功的用于 RAW264.7细胞提取液中过氧化氢的检测。本方法在 55s内完成了对PMAJ 激的RAW264.7细胞提取液中过氧化氢的检测，测得的结果与文献报道相符。这项应用为测 定其他生物样品中的过氧

16、化氢提供了一种思路。在第三章中，基于第二章中已建立的分析体系，在进样通道两侧设计有辅助通道的双T形芯片上，对单个 RAW264.7细胞在微流控芯片上的进样、分离及过氧化氢的测定进行了探索试验，初步实现了微流控芯片上单个 RAW264.7巨噬细胞中过氧化氢的测定。Science Direct目的：为了解国外的研究动向及现状，做由查询。JooHeon Lee, Buu-Chau Huynh-Nguyen, EunaKo, JiHye Kim, Gi Hun Seong, Fabrication of flexible, transparent silver nanowire electrodes

17、for amperometric detection ofhydrogen peroxide, Sensors and Actuators B: Chemical, Volume 224, 1 March 2016, Pages 789-797, ISSN 0925-4005, http:/10.1016/j.snb.2015.11.006.( HYPERLINK /science/article/pii/S0925400515305840 /science/article/pii/S0925400515305840)Abstract: AbstractWepresent a simple m

18、ethod to fabricate flexible, transparent silver nanowire (AgNW) films. HomogenousAgNWnetworks were formed on a mixed cellulose ester (MCE) membrane by vacuum filtration and were easily transferable to self-adhesive poly(ethyleneterephthalate). The opaque, white MCE membrane became transparent after

19、being subjected to hot acetone vapor. The fabricated AgNWfilms had an average resistivity of 13 Q/sq and a transmittance of approximately 67%. Moreover, the AgNWfilms showed excellent mechanical properties in repeated adhesion tests and bending tests. The AgNWfilms also showed good resistance agains

20、t heat and NaCl solutions. AgNWfilms were patterned by a combination of soft lithography and a solution-based chemical etching technique. The area under the photoresist polymer maintained its conductivity and transmittance after etching, whereas increased resistance and transparency were observed in

21、 the etched area. The suitability of the patterned AgNW electrodes for electronic devices was demonstrated via a simple LED chip array. After using standard photolithography to define the working area, AgNW films were used as sensor electrodes for the electrochemical detection of hydrogen peroxide (

22、H2O2). The AgNWsensors displayed a reasonable detection limit of 46 M (S/N = 3), a rapid response time (within 2s), and high sensitivity (749 科 AmM 1 cm-2 and 1640 科 A mM-1 cm- 2). Furthermore, the AgNWsensor was resistant to other potential interfering electroactive species commonly present in phys

23、iological samples such as l-ascorbic acid, glucose, and sodium oxalate. These results indicate that the AgNW sensor is selective for electroreduction of H2O2. Additionally, the developed sensor exhibited a stable amperometric response to the reduction of H2O2even after extended storage.Keywords: Sil

24、ver nanowire; Hydrogen peroxide; Electrochemical analysis; Flexible electrode; Micro-patterningJian Liu, Xiangjie Bo, Zheng Zhao, LipingGuo, Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells, Biosensors and Bioelectronics,

25、 Volume 74, 15 December 2015, Pages 71-77, ISSN 0956-5663, http:/10.1016/j.bios.2015.06.042. ( HYPERLINK /science/article/pii/S0956566315302104 /science/article/pii/S0956566315302104) Abstract: AbstractIn this study, we developed a novel biosensor based on highly exposed Pt nanoparticles (Pt NPs) de

26、corated porous graphene (PG) for the reliable detection of extracellular hydrogen peroxide (H2O2) released from living cells. The commercially available low-cost hydrophilic CaCO3spheres were used as template for preparing PG. The porous structure provided larger surface area and more active sites.

27、Due to the porous structure of PG, the Pt NPs supported on PG were not secluded by aggregated graphene layers and were highly exposed to target molecules. Ultrafine Pt NPs were well dispersed and loaded on PG by a method of microwave assistance. Electrochemical performances of the Pt/PG nanocomposit

28、es modified glassy carbon electrode (GCE) were investigated. The electrocatalytic reduction of H2O2 showed a wide linear range from 1 to 1477 科 M, with a high sensitivity of341.14 AmM-1 cm- 2 and a limit of detection (LOD) as low as 0.50科 M. Moreover, the Pt/PG/GCEexhibited excellent anti-interferen

29、ce property, reproducibility and long-term storage stability. Because of these remarkable analytical advantages, the constructed sensor was used to determine H2O2 released from living cells withsatisfactory results. The superior catalytic activity makes Pt/PG nanocomposites a promising candidate for

30、 electrochemical sensors and biosensors design. Keywords: Porous graphene; Pt nanoparticles; Cell tracking; Electrochemical biosensor; Nonenzymatic hydrogen peroxide detectionH. Heli, J. Pishahang, H. BarzegarAmiri, Synthesis of hexagonal CoAl-layered double hydroxide nanoshales/carbon nanotubes com

31、posite for the non-enzymatic detection of hydrogen peroxide, Journal of Electroanalytical Chemistry, Volume 768, 1 May 2016, Pages 134-144, ISSN 1572-6657, http:/10.1016/j.jelechem.2016.01.042.( HYPERLINK /science/article/pii/S1572665716300418 /science/article/pii/S1572665716300418)Abstract: Abstrac

32、tA nanocomposite comprising hexagonal CoAl-layered double hydroxide nanoshales with multiwall carbon nanotubes (MWCNTs) was synthesized by reflux heating. The nanocomposite was then applied to modify a carbon paste electrode. The modified electrode was then applied to fabricate a non-enzymatic hydro

33、gen peroxide sensor.Hydrogen peroxide was electrocatalytically oxidized on the modified electrode surface, while its electroreduction was performed by an enhanced direct electron transfer to the electrode surface. The catalytic rate constants of the hydrogen peroxide electrooxidation were processed

34、by an active cobalt species, and the diffusion coefficient of hydrogen peroxide was reported. An amperometric method was developed for determination of hydrogen peroxide based on its electrocatalyticoxidation with a sensitivity of 118.0 mA dm3 mol- 1 cm - 2 and a limit of detectionof 10moldm- 3. In

35、addition, amperometric method based on hydrogen peroxideelectroreduction had a sensitivity of 42.0 mA dm3 mol- 1 cm - 2 and a limit ofdetection of 40 科 moldm- 3. The sensor had the advantages of high activity and sensitivity, with a simple fabrication method without complications of immobilization s

36、teps and using any enzyme or reagent.Keywords: CoAl-layered double hydroxide; Multiwall carbon nanotubes; Hydrogen peroxide; ElectrocatalysisWeb of SciencePtW/MoS 2 hybrid nanocomposite for electrochemical sensing ofH 2O 2 released from living cells作者：Lilian Zhu ; Yuan Zhang; Pengcheng Xu; Weijia We

37、n ; XinxinLi; Jiaqiang XuBiosensors and Bioelectronics卷：80页：601-6DOI: 10.1016/j.bios.2016.02.019出版年：15 June 2016 摘要Hydrogen peroxide (H2O 2) as an important reactive oxygen species (ROS) isreactive and potentially harmful to cells, causing oxidation of lipids, proteins and DNA. Herein, we report a P

38、tW/MoS 2 hybrid nanocomposite with ultrasensitive and highly specific response for thedetection ofH 2O 2 released from breast cancer 4T1 cells. Upon exposure to 5 nMof H 2O 2, the electrochemical response is still visible. This PtW/MoS2 hybridnanocomposite could be facilely synthesized through in-si

39、tu growth of PtWnanocrystals on the surface of MoS2 nanosheets. The incorporation ofPtWnanocrystals and MoS2nanosheets in conjunction with each other to formhybrid nanocomposite improves the selective interaction of H2O 2 withsensing material surface, and further increases the sensitivity and select

40、ivity of sensor. All rights reserved ElsevierAdsorptive Removal of Al, Zn, Fe, Cr and Pb from Hydrogen Peroxide Solution by IR-120 Cation Exchange Resin 作者：Ghanbari Pakdehi, S (GhanbariPakdehi, Shahram) 1 IRANIAN JOURNAL OF CHEMISTRY & CHEMICAL ENGINEERING-INTERNATIONAL ENGLISH EDITION 卷：35期：1页：75-8

41、4出版年：WIN 2016 查看期刊信息 摘要Adsorption of cations Al, Zn, Fe, Cr and Pb from aqueous solution of hydrogen peroxide using IR-120 cation-exchange resin was studied. The removal percentage of the cations was examined by varying experimental conditions (such as pH of thehydrogen peroxide solution, temperatur

42、e,contact time and dosage of adsorbent) in a stirred-batch system. Under the optimal conditions (pH=1, T=25 degrees C, contact time=150 min, adsorbent dosage=5g), it was found that more than 98% removal was achieved. The cations adsorption was studied on the basis of Langmuir, Freundlich and linear

43、isotherms in the concentration range of 0-50 mg/L. Adsorption of the cations on the resin followed first-order, pseudo-first-order (Lagergren) and intraparticle diffusion kinetic models in the cations range of 10-20 mg/L. The best fitted model for kinetic model was obtained as Lagergren model. The o

44、btained studies showed that IR-120 cation-exchange resin was successfully used as an efficient adsorbent for removal of the cations from aqueous solution of hydrogen peroxide.A highly selective fluorescent turn-on NIR probe for the bioimaging of hydrogenperoxide in vitro and in vivo作者：Wang, P (Wang, Peng) 1 ; Wang, K (Wang, Ke) 1 ; Gu, YQ (Gu, Yueqing) 1 SENSORS AND ACTUATORS B-CHEMICAL 卷：228页：174-179DOI: 10.1016/j.snb.2016.01.025 出版年：JUN 2 2016 查看期刊信息 摘要A turn-on NIR fluorescent probe for sensitive detection of hydrogenperoxide was developed. After the additionof hydrogenper