RF9 - Neuropeptide Y Receptor Antagonist - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemERF9Cat. No.: HY-107382CAS No.: 876310-60-0分式: CHNO分量: 482.62作靶点: Neuropeptide Y Receptor作通路: GPCR/G Protein; Neuronal Signaling储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 150 mg/mL (310

2、.80 mM; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.0720 mL 10.3601 mL 20.7202 mL5 mM 0.4144 mL 2.0720 mL 4.1440 mL10 mM 0.2072 mL 1.0360 mL 2.0720 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 RF9种有效且有选择性的神经肽FF受体 (Neuropeptide FF receptor)

3、 拮抗剂 ，对于 hNPFF1R 和 hNPFF2R的 Ki 值分别为 585 和 759 nM。IC50 & Target Ki: 585 nM (hNPFF1R), 759 nM (hNPFF2R) 1体内研究RF9 (10 g) infused alone does not result in a significant alteration of MAP or heart rate. Conversely, MAPand heart rate increases evoked by NPFF are significantly blocked when NPFF is applied

4、in conjunction with1/2 Master of Small Molecules 您边的抑制剂师www.MedChemERF9 1. It is worthy to note that RF9 (30 nmol) significantly reduces the hypothermia induced by morphine (5nmol) 2.PROTOCOLAnimal Rats: Rats (n=5) are catheterized for measurement of arterial blood pressure and receive an in-dwellin

5、gAdministration 1 cannula into the lateral cerebral ventricle. Arterial blood pressure and heart rate are continuously monitored.NPFF (10 g in 10 L of saline) is injected into the lateral ventricle over 15 to 20 sec. Control injections ofsaline (10 L) are carried out in the same animals. Upon return

6、 of blood pressure to baseline, RF9 (10 g) isinjected i.c.v., followed by another i.c.v. injection of NPFF with RF9. After 1.5 h., another i.c.v. infusion ofNPFF is repeated 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Simonin F, et al

7、. RF9, a potent and selective neuropeptide FF receptor antagonist, prevents opioid-induced tolerance associated withhyperalgesia. Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):466-71.2. Wang YQ, et al. Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice. Peptides. 2008Jul;29(7):1183-90.McePdfHeightCaution: Product has not been fully validated for medical applications.For research