高通量测序培训班8-高通量测序仪研究现状与发展

1、2018 BGI-Shenzhen All Rights Reserved2005200620072008200920102011201220132014201520162017Solexa GA: 1G:35 bpSolexa GAII: 5G:50 bpSolexa GAIIx: 50G:75 bpHiseq2000: 200G:100 bpHiseq2500: 600G:100 bpNextseq 500: 120G:150 bpHiseq 3000/4000: 750/1500G:150 bpNovaSeq: 3000G: 150 bpMiseqHiseq X TenMiniseq:

2、8.5G:250 bp: 1800G:150 bp: 7.5G: 150 bpSOLiD 1: 1G:25 bpSOLiD 4: 100G:50 bpIon PGM 314: 10Mb:100 bpIon PGM 318: 1G:200 bpIon Proton PII: 100G:200 bpSOLiD 2: 4G:35 bpSOLiD 5500 xl: 160G:60 bpIon Proton PI: 10G:200 bpMGISEQ-200: 60Gb: 100 bpIon S5: 50G:600 bpBGISEQ-500: 520Gb: 100 bpBGISEQ-50: 8Gb: 50

3、 bpGS 20: 20 Mb:100 bpGS FLX: 100Mb: 250 bpGS FLX Titanium: 450Mb: 500 bpGS Junior: 35Mb: 400 bpGS FLX +: 500Mb: 700 bpGS FLX +: 700Mb: 700 bpMGISEQ-2000: 1000Gb: 150 bpBGISEQ-1000: 2000Gb: 50 bpRevolocity: 15000Gb: 50 bp2018 BGI-Shenzhen All Rights ReservedLIFEILMNBGIRCA/DNBPolony PCROn a slideEmul

4、sion PCR(Illumina)(SOLiD)(Ion Torrent)(cPAS)(454)(cPAL)2018 BGI-Shenzhen All Rights ReservedtcRocheuytcntcioabgNuytcstc11,1101,1.1pfRSehtc1105 / 415GrqmRPTl1105 5041-5.5.22018 BGI-Shenzhen All Rights ReservedJTPRSTPO 1LMSample Input and FragmentationLibrary PreparationOne Fragment = One BeademPCR Am

5、plification+2,F2+252U+2,F2 F+252 F1F 3.GF/FF.GF101F.GF1 .7 .7Sequencing: One Bead = One ReadData Processing and Analysis 7 4 72018 BGI-Shenzhen All Rights ReservedIlluminaANtNltFS re,w,BlsoCDuctso 2018 BGI-Shenzhen All Rights ReservedRSa18G38G38G4M8G0IG0IG4L8GTX7 98G38G(P(HI3IG4M8G0IG0IG4L8G.P)NI08G

6、917.5 hours(P)HIPHI 08G ( HIP*NI.NIP(HI08G ) 3M5/)/)/)/*/3M7I6H7( )()*3M72O )O )O O )O )O )O )/B2/g2/bP(Puiescnomns4B 354EBB 4135(-0 4BcTft q8.,8ns )i%lX -Sdpis 1 ks6taX2018 BGI-Shenzhen All Rights Reserved IMlac25797.7878257.S488qlabfs- $257. 34257. 3400257. 48834P,$e$xiubfxG$-RnG$,o-$16euG/6xG,$Rn

7、G$-$o34 00$B$B,$euG$/6xGRnG-,o488$-$2018 BGI-Shenzhen All Rights Reservedk.4 15 055A ,4D.0.4 15 055A ,4o(ehfekLrpdmDic:2(:2ngk(a boxNkRmNnbDtls/OakTA)1552018 BGI-Shenzhen All Rights Reservedufp9CM 8LMBbMIBO598FB32BMIBO598 )-32BN7G7F598ufdlncnc(%nc)%L Uio%L U%L Utxhgm% U6%IFN.%IFNIFPOB )-PMNS,MN(U6T

8、)%IFN.%IFNIFPOB )-PMN0,MN(U6T .%IFN.%IFNIFPOB )-PMN0,MNmtsearlkMBNlIFPOBNIFPOBNIFPOBN7)7(777%7)-lk4)4-441NBFw=LO.=LO.=LO.2018 BGI-Shenzhen All Rights ReservedFMw, 296Dh, 296nOpon.woDtiasoC,w.DDcDoDu A999 0.1eNGgnS mnTooGs/1 P/.2lr 6 :2018 BGI-Shenzhen All Rights ReservedrifMgGifu%pNqlroabr26 32A6 23

9、2%. 6GG :BO6:4C :B 4COPskdsknskkd 6Gskgitfskfdskffdhskxsk259%Du%D %Du%D%D81CG/% (%0k(%)%lo%559Msa).)%4(%5CCGsa)%481CG-%-rar(cu6:-p3%k3-%kuqdduqduqdO9M(TePSqbbSq2018 BGI-Shenzhen All Rights Reserved2018 BGI-Shenzhen All Rights Reserved)()( NGS OxfordPacBio RS NanoporeMinION ABI3730Thermo Fisher(Life

10、Tech)IlluminaMGIABI3500 S SMRTB1,000-10,000bp5,400bp400-900bp 50-150bp(x2)600bp50-150bp (x2)0.2Mb/run 60-3,000Gb/run 30-50Gb/run 20-5,000Gb/run 0.5-9Gb/run 30-400bp/99%99%99%99%90-95%90-95%BBBG2018 BGI-Shenzhen All Rights ReservedKYSNaKY)05TR )05TR )05TR 148(600(IRCH-(cQ HFC0GA 0BA4DBFBA8DB-ED )05TR OEMOEM(I)BFDBACIPHFC IeHFC (6M 2018 BGI-Shenzhen All Rights ReservedqhOiqhQxlTqhQeono1-7K1-71-7aPx1-731-7K31-702681111PxNucYtcRPx4HE 4M1AAIBCS5-3MFBD .GFS5-3MFBD .GFS5-3M1AAIBCS5-3DAHFDCE 4HE 8rmDFIAFCDF