分析化学1光盘2

1、Chromatography is anytical methodt is widely used for theseparation, identification and determination of the chemical components incomplex mixtures. No other separation method is asapplicable as chromatography.erful and generallyChromatography was invented by Russian botanist Mikhail Tstheturn of th

2、e 20 century. Various plant pigments were loaded on the glass columns packed with calcium carbonate, then separated and eluted by petroleum ether. The separated species appeared as colored bands on the column, which accounts for the name of the method (Greek chroma meaning color and graphein meaning

3、 to write). Up to now, chromatography is difficult to define rigorously because the term has been app d to such a variety of systems and techniques. However, all of these methods have a common placehe use of the s ionary phase and the mobile phase.The sionary phase in chromatography is the phase whi

4、ch is fixed inplace either in a column or on a planar surface. The mobile phase is the phasewhich moves through the sionary phase, carrying theyte with it.Components of a mixture are loaded through the sionary phase and elutedby the flow gaseous or liquid mobile phase. Finally, separation occurs bec

5、ausesle components have different affinities betn the sionary and mobilephase, and they have different migrant rates and different retention times.第十六章 色谱分析法概论chromatography 色谱法sionary phase 固定相mobile phase elution 洗脱chromatogram 色谱图baseline 基线 peak 色谱峰 tailing peak 拖尾峰leading peak 前延峰symmetry facto

6、r 对称因子相tailing factor 拖尾因子 retention time 保留时间 dead time 死时间adjusted retention time 调整保留时间retention volume 保留体积dead volume 死体积adjusted retention volume 调整保留体积relative retention 相对保留值 retention index 保留指数 Kovats index; Kovats 指数 peak height 峰高peak area 峰面积standard deviation 标准差peak widpeak width 峰宽lf

7、 height 半峰宽resolution 分离度distribution coefficient 分配系数 partition coefficient 分配系数 retention factor 保留因子gas chromatography ; GC 气相色谱法liquid chromatography ; LC 液相色谱法supercritical fluid chromatography ; SFC 超临界流体色谱法 bonded-phase chromatography ; BPC 键合相色谱法 column chromatography 柱色谱法planar chromatograp

8、hy ; plane chromatography 平面色谱法capillary electrophoresis ; CE 毛细管电泳法packed column 填充柱capillary columnmicrobore packed column 微填充柱open tubular column 开管柱high performance liquid chromatography ; HPLC 高效液相色谱法pr chromatography 纸色谱法thin layer chromatography ; TLC 薄层色谱法thin film chromatography 薄膜色谱法partit

9、ion chromatography 分配色谱法partition coefficient 分配系数normal phase chromatography 正相色谱法 reversed phase chromatography 反相色谱法 adsorption chromatography 吸附色谱法 adsorption coefficient; Ka 吸附系数solvent strength; o 溶剂强度ion exchange chromatography ; IEC 离子交换色谱法ion exchanger 离子交换剂resin 树脂molecular exclusteric exc

10、luchromatography; MEC 分子排阻色谱法chromatography ; SEC 空间排阻色谱法gel chromatography 凝胶色谱法gel permeation chromatography ;GPC 凝胶渗透色谱法 gel filtration chromatography ;GFC 凝胶过滤色谱法 permeation coefficient 渗透系数plate theory 塔板理论 rate theory 速率理论 number of plates 塔板数plate height (height equivalent to a theoretical plate); H 塔板高度effective number of plates; neff 有效板数effective plate height; Heff 有效塔板高度eddy diffu涡流扩散packing factor 填充因子longit