埃希菌属菌-微生物学教学课件

1、Medical MicrobiologyDepartment of Microbiology, HMUDepartment of Microbiology, Harbin Medical University第二篇 致病性细菌第14章埃希菌属Escherichia微生物学教研室 张文莉Department of Microbiology, Harbin Medical University教学大纲熟悉肠道杆菌的共同特征及分类致病性大肠埃希菌的种类和致病机制掌握大肠埃希菌的卫生细菌学检查及其意义了解大肠埃希菌的微生物学检查法Department of Microbiology, Harbin M

2、edical University问题大肠埃希菌与人类健康的关系如何？肠道杆菌的共同特征？Department of Microbiology, Harbin Medical University形态染色0.4-0.7m 1-3m 短杆菌革兰染色阴性有鞭毛有菌毛Department of Microbiology, Harbin Medical UniversityDepartment of Microbiology, Harbin Medical University培养特性与生化反应兼性厌氧营养要求不高生化反应活泼，发酵多种糖类绝大多数发酵乳糖IMViC试验结果为+ + - -Depart

3、ment of Microbiology, Harbin Medical University复杂的抗原构造Department of Microbiology, Harbin Medical University致病物质菌毛、定居因子抗原K抗原 与侵袭力有关抗吞噬与抗补体的溶菌外毒素 肠毒素及溶血毒素内毒素与感染特征和严重程度有关Department of Microbiology, Harbin Medical University所致疾病肠道外感染 泌尿道感染最多见 化脓性感染腹泻Department of Microbiology, Harbin Medical UniversityD

4、epartment of Microbiology, Harbin Medical UniversityDepartment of Microbiology, Harbin Medical University肠侵袭型大肠埃希菌（EIEC）致病物质不产肠毒素，侵袭结肠粘膜上皮细胞所致疾病主要侵犯较大儿童和成人腹泻呈脓血便，有里急后重诊断EIEC无动力、生化反应和抗原结构Department of Microbiology, Harbin Medical UniversityDepartment of Microbiology, Harbin Medical University肠出血型大肠埃希

5、菌（EHEC）亦称为vero毒素大肠埃希菌 致病物质菌毛：粘附作用毒素：vero毒素所致疾病出血性结肠炎溶血性尿毒综合征Department of Microbiology, Harbin Medical UniversityIntimate Adherence of EHEC to Epithelial CellsDepartment of Microbiology, Harbin Medical UniversityThe “life cycle” of E. coli O157:H75-10% prevalence in animals40% prevalence in farmsDep

6、artment of Microbiology, Harbin Medical University 肠集聚型大肠埃希菌 EAggEC致病物质毒素粘附素所致疾病引起婴儿持续性腹泻，脱水，偶有血便Department of Microbiology, Harbin Medical University肺泡巨噬细胞吞噬大肠埃希菌Department of Microbiology, Harbin Medical University 微生物学检查法标本 分离培养与鉴定肠道外感染肠道内感染 Department of Microbiology, Harbin Medical University卫生细菌学检查大肠菌群指数每1000(g)ml水中大肠菌群数大肠菌群细菌总数 每1ml(g)样品中细菌总数我国卫生标准大肠菌群细菌总数Department of Microbiology, Harbin Medical University防治原则疫苗抗生素的抗性质粒，耐药性非常普遍Department of Microbiology, Harbin Medical University小结肠道杆菌的共