索取part与提交part流程

1、art与提交part流程帮助文档http:/Help:Contents11 Distribution KitsThe 2012 Distribution contains over 1000 part samples as dried (miniprepped) DNA, with each sample QC tested through sequencing, AB test plates, and restriction digests.http:/Help:Distribution_Kits2 1.1 2012年包含(5) 384w DNA Distribution Kit Plate

2、s Linearized Plasmid Backbones: pSB1A3, pSB1C3, pSB1K3.m1, pSB1T3 iGEM Stickers and Pins! (iGEM teams only) 31.2 Protocols（未看）3A Assembly KitCompetent CellsTransformationMiniprepRestriction DigestLigationLinearized Plasmid Backbones42 搜寻part搜寻basic part搜寻composite part搜寻部分part52.1 搜寻basic partpart n

3、umbername of the partbrowse for parts by typebrowse parts by function帮助文档6part number789name of the part101112browse for parts by type13browse parts by function142.2 搜寻composite part 与搜寻basic part方法一样151.3 How to find previously assembled parts that will aid in constructing a composite part 163 索取pa

4、rt帮助文档17 3.1 搜寻18Available代表可以索取19不可索取20213.2 Quality Control质量控制帮助文档：22The Get This Part page is extremely useful when requesting a part sample. It will tell you: if your part sample is already included in your Distribution Kit if there are multiple samples for the part in the Registry any QC infor

5、mation tied to the part sample 23You can use the following information to email a request to hq (at) igem . org. The subject should be along the lines of Part Request: Your Teams Name. Be sure to include the following: iGEM team or lab name part name (BBa.) plasmid backbone and resistance sample loc

6、ation: source plate and well quality control information (if applicable): Sequence and Gel Result 24Things to keep in mindMany of the part samples available through the Registry have undergone quality control testing. Prior to requesting a part sample, take a look at this information, as it should a

7、ffect your decision on using it. See the quality control help page if you need help interpreting QC results on the “Get This Part” page. 在申请之前看清信息Prior to making a request it is helpful to consider all of the parts that the team needs for your project. We prefer bundling part requests together rathe

8、r than receiving several small requests, as this cuts down shipping costs and improves efficiency. 所有元件尽量一起索取Once a request is made you should be contacted by iGEM HQ within two business days. IGEM总部在两天内会联系你253.3 Using agar stabs（琼脂穿刺）We send out our part requests as agar stabsThe agar should alread

9、y have a hole from when it was stabbed. With an inoculating loop dipped into the stab, you can plate directly onto a petri dish with the appropriate antibiotic.Incubate the dish overnight at 37C (14-16hr)Pick a single colony to start up a cultureMiniprep to extract plasmid DNA 26实验组实现了解与准备好转移与保留培养流程

10、274 注册新part帮助文档http:/Help:Adding_Parts28在做出part前先在Registry注册http:/Add_a_Part_to_the_Registry 添加文件29Add a Basic Part305 构建part帮助文档：http:/partsr/Help:Constructing_Parts/wiki/The_BioBricks_Foundation:Standards/Technical/Formats31 5.1 BioBrick所谓生物模块（ BioBrick）是指经标准化加工，具有标准的限制酶切割位点的元件、装置和系统等。它也可以形象地称为“生物

11、砖” 合成生物学与合成酶学.张金等.科学出版社32标准化加工所谓标准化加工，是指所设计和构建的元件、装置和系统能够协调匹配，规范连接，实现即插即用。元件需要用应用前缀和后缀序列，其含有限制酶Ecor 、Xba 、Spe 和Not 切割位点。元件的核苷酸序列中不应含上述限制酶的切割位点。 合成生物学与合成酶学.张金等.科学出版社335.2 BioBrick RFC10标准化标准之一： BioBrick RFC 10（比赛规定）帮助文档： http:/Help:Standards/Assembly/RFC10其它标准化过程：http:/wiki/The_BioBricks_Foundation:R

12、FC345.3 Basic Part 标准化前缀序列(有酶切位点）+part（需要标准化的序列）+后缀序列（有酶切位点）355.3.1 编码蛋白质区域的标准化365.3.2 其他序列的标准化375.4 Basic Part 标准化方法PCR标准化http:/Help:Protocol/PCR_StandardizationDNA合成http:/Help:Protocol/DNA_Synthesis定点诱变：改变序列中的illegal restriction sites385.5 Composite Parts标准化3A AssemblyScarless AssemblyGibson AssemblyStandard Assembly39提交part(邮寄）The Registry only accepts part samples in pSB1C3, the Registrys plasmid backbone shipping standard帮助文档：4041424344454647*Important*: When shipping your samples, make sure to include a detailed desc