N1-Methylpseudouridine-1-Methylpseudouridine-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•Agonists•ScreeningLibrarieswww.MedChemEN1-MethylpseudouridineCat.No.:HY-112582CASNo.:13860-38-3Synonyms:1-Methylpseudouridine分⼦式:C₁₀H₁₄N₂O₆分⼦量:258.23作⽤靶点:Others作⽤通路:Others储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:125mg/mL(484.06mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.8725mL19.3626mL38.7252mL5mM0.7745mL3.8725mL7.7450mL10mM0.3873mL1.9363mL3.8725mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存⽅式和期限。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案，配制前请先配制澄的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的⼯作液，建议您现⽤现配，当天使⽤；澄的储备液可以根据储存条件，适当保存；以下溶剂前的百分⽐指该溶剂在您配制终溶液中的体积占⽐)：1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(8.05mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(8.05mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoil1/2MasterofSmallMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.08mg/mL(8.05mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性N1-methyl-pseudouridine(1-Methylpseudouridine)⼀种甲假尿苷，翻译性能优于5mC和5mC/N1-methyl-pseudouridine。通过增加核糖体密度，mRNA中的N1-methyl-pseudouridine以eIF2α依赖性和独⽴机制增强翻译。体外研究IncorporationofN1-methyl-pseudouridineintomRNAmodifiesmRNAsproducedhigheramountsoflucthanthestandardLucmRNAinHEK293Tcells.IncorporationofN1-methyl-pseudouridinenucleosidemodificationinbothLucandGFPmRNAenhancestheinitiationstepoftranslation,inpartbysuppressingeIF2αphosphorylation.Inaddition,polysomeformationandgrowthontheNN1-methyl-pseudouridine-containingLucmRNAisenhancedduetothereductionofelongationrate.Inalltheinvitrotranslationsystems,incorporationofN1-methyl-pseudouridineinLucandGFPmRNAsdramaticallyenhancedtranslation.TheN1-methyl-pseudouridine-LucmRNAisassociatedwithheavierpolysomesthanLucmRNA[1].体内研究N1-methylpseudouridine-incorporatedmRNAoutperformspseudouridine-incorporatedmRNAbyprovidingenhancedproteinexpressionandreducedimmunogenicityinmammaliancelllinesandmice[2].N1-methyl-pseudouridine(1-Methylpseudouridine)(20μg;I.m.ori.d.routesfor21days)andm5C/N1-methyl-pseudouridine-modifiedmRNArespectivelyhaveahighertranslationalcapacitythanΨandm5C/Ψ-modifiedmRNAinvivo[2].AnimalModel:7-week-oldBalb/cmice[1]Dosage:20μgAdministration:I.m.ori.d.routesfor21daysResult:hadahighertranslationalcapacity.REFERENCES[1].SvitkinYV,etal.N1-methyl-pseudouridineinmRNAenhancestranslationthrougheIF2α-dependentandindependentmechanismsbyincreasingribosomedensity.NucleicAcidsRes.2017Jun2;45(10):6023-6036.[2].AndriesO,etal.N(1)-methylpseudouridine-incorporatedmRNAoutperformspseudouridine-incorporatedmRNAbyprovidingenhancedproteinexpressionandreducedimmunogenicityinmammaliancelllinesandmice.JControlRelease.2015Nov10;217:337-44.McePdfHeightCaution:Producthasnotbeenfullyvalidatedf