TC11-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemETC11Cat.No.:HY-129478CASNo.:100823-03-8分⼦式:C₂₀H₂₂N₂O₂分⼦量:322.4作⽤靶点:Caspase;Bcl-2Family;CDK作⽤通路:Apoptosis;CellCycle/DNADamage储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:50mg/mL(155.09mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.1017mL15.5087mL31.0174mL5mM0.6203mL3.1017mL6.2035mL10mM0.3102mL1.5509mL3.1017mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(7.75mM);Clearsolution1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性TC11⼀种MCL1降解剂和Caspase-9和CDK1激活剂。TC11作为苯酞酰亚胺衍⽣物在结构上与免疫调节药物有关。在长时间有丝分裂阻滞期间，TC11诱导MCL1降解导致凋亡性死亡[1][2]。IC50&TargetCDK1Caspase-9MCL1体外研究TC11(0~30μM;24hours;KMS34cells)inducescelldeathinKMS34[1].TC11(5μM;0~48hours;KMS34cells)inducescelldeathoccursthroughanapoptoticpathwayanddownregulatesMCL1expression[1].TC11(5μM;24hours;KMS34cells)inducesMarrest[1].CellViabilityAssay[1]CellLine:KMS34cellsConcentration:0~30μMIncubationTime:24hoursResult:Inducedcelldeath.WesternBlotAnalysis[1]CellLine:KMS34cellsConcentration:5μMIncubationTime:0~48hoursResult:InducedcelldeathoccursthroughanapoptoticpathwayanddownregulatedMCL1expression.CellCycleAnalysis[1]CellLine:KMS34cellsConcentration:5μMIncubationTime:24hoursResult:InducedMarrest.REFERENCES[1].IchikawaD,etal.Aphenylphthalimidederivative,TC11,inducesapoptosisbydegradingMCL1inmultiplemyelomacells.BiochemBiophysResCommun.2020;521(1):252-258.[2].ShiheidoH,etal.Aphthalimidederivativethatinhibitscentrosomalclusteringiseffectiveonmultiplemyeloma.PLoSOne.2012;7(6):e38878.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchus