Chrysin-5-7-Dihydroxyflavone-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEChrysinCat.No.:HY-14589CASNo.:480-40-0Synonyms:5,7-Dihydroxyflavone分⼦式:C₁₅H₁₀O₄分⼦量:254.24作⽤靶点:EstrogenReceptor/ERR作⽤通路:Others储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥100mg/mL(393.33mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM3.9333mL19.6665mL39.3329mL5mM0.7867mL3.9333mL7.8666mL10mM0.3933mL1.9666mL3.9333mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(9.83mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(9.83mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Chrysin⼀种雌激素阻断剂。IC50&Targetestrogen体外研究Chrysinismainlyfoundinpassionflowers,honey,andpropolisactsasapotentialtherapeuticandpreventiveagenttoinhibitproliferationandinvasionofvarioushumancancercells.AlthoughChrysinhasanti-carcinogeniceffectsinseveralcancers,littleisknownaboutitsfunctionalrolesinovariancancerwhichshowspoorprognosisandchemoresistancetotraditionaltherapeuticagents.Chrysininhibitsovariancancercellproliferationandinducedcelldeathbyincreasingreactiveoxygenspecies(ROS)productionandcytoplasmicCa2+levelsaswellasinducinglossofmitochondrialmembranepotential(MMP).ChrysinactivatesMAPKandPI3K/AKTpathwaysinES2andOV90cellsinconcentration-responseexperiments.ChrysinsuppressestumorgrowthbyregulatingcanonicalWntandnuclearfactorNF-κBsignalingcascadescancercells.ChrysinstimulatesthephosphorylationofAKTandP70S6KproteinsinbothES2andOV90cellscomparedtotheuntreatedcontrolcells.Inaddition,Chrysinactivatesthephospho-ERK1/2,p38,andJNKproteinsasmembersoftheMAPKpathwayintheovariancancercells[1].PROTOCOLCellAssay[1]Theproliferationassaysareconductedusingacellproliferationenzyme-linkedimmunosorbentassay(ELISA)5-bromo-2'-deoxyuridine(BrdU)kit.Briefly,ES2andOV90cellsareseededina96-wellplate,andthentreatedwithChrysin(0,5,10,20,50,and100µM)withorwithoutinhibitors(20μMLY294002,PI3K/AKT;10μMU0126,ERK1/2;10μMSP600125,JNK;and20μMSB203580,p38)inafinalvolumeof100μL/well.Aftera48-hincubation,10μMBrdUisaddedtothecellculture,followedbyanadditional2-hincubationat37°C.AfterlabelingthecellswithBrdU,theyarefixedandthenincubatedwiththeanti-BrdU-peroxidase(POD)workingsolutionfor90min.Theanti-BrdU-PODbindstotheBrdUincorporatedintonewlysynthesizedcellularDNAandtheseimmunecomplexesaredetectedbyanalyzingtheirreactionwiththe3,3',5,5'-tetramethylbenzidine(TMB)substrate.Theabsorbancevaluesofthereactionproductaremeasuredat370and492nmusinganELISAreader[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•ActaPharmSinB.2021Jan;11(1):143-155.•ClinSci.2021Jul20;CS20210571.•IntJMolMed.July13,2021.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE•CellCycle.2022Jan5;1-13.•RegenTher.13January2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LimW,etal.ChrysinAttenuatesProgressionofOvarianCancerCellsbyRegulatingSignalingCascadesandMitochondrialDysfunction.JCellPhysiol.2017Aug17.McePdfHeightCautio