LCL521-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemELCL521Cat.No.:HY-103593CASNo.:1226851-11-1分⼦式:C₃₁H₅₂N₄O₇分⼦量:592.77作⽤靶点:Phospholipase作⽤通路:MetabolicEnzyme/Protease储存⽅式:Pureform-20°C3yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:165mg/mL(278.35mM;Needultrasonic)Ethanol:100mg/mL(168.70mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.6870mL8.4350mL16.8699mL5mM0.3374mL1.6870mL3.3740mL10mM0.1687mL0.8435mL1.6870mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.75mg/mL(4.64mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.75mg/mL(4.64mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.75mg/mL(4.64mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性LCL521⼀种酸性神经酰胺酶(ACDase)抑制剂。LCL521还抑制溶酶体酸性鞘磷脂酶(ASMase)。IC50&TargetACDase,ASMase[1]体外研究LCL521(1µM)actsasapotentinhibitorofcellularACDaseactivity,whereas10µMLCL521hasanadditional,decreasedaffectontheα-formofthisenzyme.LCL521(10µM)causesatime-dependent(1hoursand5hours)decreaseoftheα-ACDaseforminMCF7cells[1].REFERENCES[1].BaiA,etal.Targeting(cellular)lysosomalacidceramidasebyB13:design,synthesisandevaluationofnovelDMG-B13esterprodrugs.BioorgMedChem.2014Dec15;22(24):6933-44.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-379