肥大细胞对THP-1巨噬细胞源性泡沫细胞胆固醇分配和流出的影响

肥大细胞对THP-1巨噬细胞源性泡

沫细胞胆固醇分配和流出的影响2021/4/261肥大细胞(MCs)一种参与炎症免疫应答反应的炎症细胞。胞浆内含有大量异染性颗粒（组胺等）。受刺激时脱颗粒,释放胞浆一系列可溶性介质到细胞外

（compound48/80、oxLDL等可以刺激MCs脱颗粒)

。研究背景2021/4/262促动脉粥样硬化(As)形成

MCs加速oxLDL诱导平滑肌细胞泡沫化。

Compound48/80处理的新西兰白兔和apoE-/-鼠主动脉脂纹形成比对照组早，面积大，分布广。2021/4/263HDLMetabolismand

ReverseCholesterolTransport胆固醇流出减少A-ICECECEFCFCLCATFCBileSR-BIA-IABCA1Mature

HDLNascent

HDLMC?ABC1=ATP-bindingcassetteprotein1;A-I=apolipoproteinA-I;

CE=cholesterylester;FC=freecholesterol;

LCAT=lecithin:cholesterolacyltransferase;

SR-BI=scavengerreceptorclassBI2021/4/264实验结果ABTheeffectofcompound48/80on

MCsdegranulation

MCswerestainedbytoluidineblue(×1000)

A，MCs；B，MCs+C48/80.

例数组胺含量（µg/ml）组胺释放率（n）颗粒释放液细胞裂解液（%）

50.340±0.0050.077±0.02181.53±4.51

MCshistaminereleasepercentage2021/4/265FormationofTHP-1macrophage-derivedfoamcells

CE/TCRatio:68.7%

HPLCanalysisofTHP-1macrophage-derivedfoamcellsOilredOstainingof

THP-1macrophage-derivedfoamcellsFC，freecholesterolCE，cholesterolesterFCCE2021/4/266

TheeffectofMCsontheaccumulationofcellcholesterolinTHP-1macrophage-derivedfoamcells

GroupsTCFCCEcontrol1092.3±65336.3±31756±60HDL3527.3±49a276.9±34250.4±17MCs891.3±62650.1±46b241.2±19bHDL3+MCs917.9±14c848.5±16c69.4±2

cC48/80807.3±68343.1±36464.2±13HDL3+C48/80314.3±10a253.2±1161.1±8Valuesarerepresentedas（）mgcholesterol/gprotein,(n=5).a:P<0.05vs.control,HDL3+MCsgroup;b:P<0.05vs.controlgroup;c:P<0.05vs.HDL3group.2021/4/267CholesterolstandardcontrolHDL3MCsHDL3+MCs

C48/80

HDL3+C48/80

ThechromatographypictureofcholesterolandcholesterolesterinTHP-1macrophage-derivedfoamcellsidentifiedbyhighperformanceliquidchromatography.2021/4/268TheeffectofMCsonthedistributionofcellfree

andesterifiedcholesterolinTHP-1macrophage-derivedfoamcells

control

(alone)=69.2%;withHDL3=47.5%;withMCs

=27.1%;withHDL3+MCs

=7.6%;withcompound48/80=57.5%;andwithcompound48/80+HDL3=19.4%.a:P<0.05vs.controlgroup;b:P<0.001vs.HDL3group.n=5.2021/4/269TheeffectofMCsoncholesteroleffluxfromTHP-1macrophage-derivedfoamcellsmediatedbyHDL3

control=2.89%;withHDL3=40.98%;withMCs

=2.91%;withHDL3+MCs

=26.92%;withcompound48/80=3.71%;andwithcompound48/80+HDL3=35.23%.

Thevaluesaremean±SD(n=5).**

P<0.01vs.HDL3+MCsgroup;#P<0.05vs.HDL3+MCsgroup;*

P<0.001vs.HDL3group.2021/4/26109、人的价值，在招收诱惑的一瞬间被决定。2023/2/32023/2/3Friday,February3,202310、低头要有勇气，抬头要有低气。2023/2/32023/2/32023/2/32/3/20234:29:20PM11、人总是珍惜为得到。2023/2/32023/2/32023/2/3Feb-2303-Feb-2312、人乱于心，不宽余请。2023/2/32023/2/32023/2/3Friday,February3,202313、生气是拿别人做错的事来惩罚自己。2023/2/32023/2/32023/2/32023/2/32/3/202314、抱最大的希望，作最大的努力。03二月20232023/2/32023/2/32023/2/315、一个人炫耀什么，说明他内心缺少什么。。二月232023/2/32023/2/32023/2/32/3/202316、业余生活要有意义，不要越轨。2023/2/32023/2/303February202317、一个人即使已登上顶峰，也仍要自强不息。2023/2/32023/2/32023/2/32023/2/3GAPDH(697bp)ABCA1(306bp)MarkercontrolHDL3MCsHDL3+MCsC48/80HDL3+C48/80*

The

effectof

MCsonABCA1mRNAexpressioninTHP-1macrophage-derivedfoamcells

AfterthetreatmentofMCs,nosignificantchangeswereshownintranscriptionlevelofcellABCA1comparedwithcontrolgroup.

Comparedwithcontrolgroup,*

P>0.05.2021/4/2612KDa3125

196A

12BSt1215%SDS[wt/vol]AGE

DegradationofHDL3andapoA-IbyMCs1,untreatedHDL3;2,MCs-treatedHDL3.2021/4/2613MCs能降解HDL3的apoA-Ⅰ；MCs能抑制HDL3介导的THP-1巨噬细胞源性泡沫细胞胆固醇流出，促使细胞内游离胆固醇蓄积。结论2021/4/2614谢谢!2021/4/26159、人的价值，在招收诱惑的一瞬间被决定。2023/2/32023/2/3Friday,February3,202310、低头要有勇气，抬头要有低气。2023/2/32023/2/32023/2/32/3/20234:29:20PM11、人总是珍惜为得到。2023/2/32023/2/32023/2/3Feb-2303-Feb-2312、人乱于心，不宽余请。2023/2/32023/2/32023/2/3Friday,February3,202313、生气是拿别人做错的事来惩罚自己。2023/2/32023/2/32023/2/32023/2/32/3/202314、抱最大的希望，作最大的努力。03二月