流式染料及应用实例介绍 Sky Kong

流式染料及应用实例介绍BD生物科学孔祥涛Sky_Kong@BDIntroductionBD是由MaxwellW.Becton和FairleighS.Dickinson

于1897年在纽约创立的，总部设在新泽西州。经过一百多年的发展，BD已成为世界上最大的医疗技术及医疗设备公司之一，它以领先的技术、卓越的产品质量和诚实可信的服务赢得了全球用户及合作伙伴的广泛赞誉。FairleghS.DickinsonMaxwellW.BectonBD生物科学作为BD的一个业务组成，一直致力于为生命科学研究者和临床医生提供创新的临床和科研工具。其主要产品包括流式细胞仪；细胞图象分析系统；单克隆抗体和试剂盒；药物研发产品及临床诊断试剂等。1973年世界第一台商品化的流式细胞仪FACSIBDIntroductionBDHLA-B27KitStemCellsTCellsResearchBDIntroductionBDIntroduction/cn/BDIntroductionFluorochromesOverviewEx-Max482nm/Em-Max695nm.NotsubjecttophotobeachinglikePerCP.NotassusceptibletofixativeorlightinstabilitycomparedtoAPC-Cy7andPE-Cy7.PerCP-Cy™5.5Ex-Max496nm/Em-Max612nm.PE-CF594isabrighteralternativetoPE-TexasRed®,withimprovedspectralcharacteristics.PE-CF594reagentsexhibitveryconsistentspillovervalueslot-to-lot,makingthemanidealchoiceforthePE-TexasRed®detector(610/20nm).BDHorizon™PE-CF594ImprovedbrightnessoverPE-TexasRedECD,andPE-AlexaFluor610BDHorizon™PE-CF594StainIndexandspillovervaluecomparison.BDHorizon™PE-CF594Ex-Max650nm/Em-Max785nm.APC-H7conjugatesprovidegreaterstabilityinlightandparaformaldehydefixativesandhavelessspilloverintotheAPCchannelthanAPC-Cy7conjugates.APC-Cy7andAPC-H7cannotbeusedsimultaneously.APC-H7BDAPC-H7demonstratedstabilityandreducedspillover.Theexperimentsshownherecomparethestability,asmeasuredby%spillover,ofwholebloodstainedwithBDAPC-H7andAPC-AlexaFluor®750conjugatedantibodies.APC-H7BrilliantViolet™421BrilliantViolet™421是一种紫色激光激发的染料，比其他紫色激光激发的染料更加明亮。并且，BrilliantViolet比蓝色和红色激光激发的染料也更明亮，它是BD提供的最明亮的染料。因此，它特别适合多色应用，可以更好地弱表达细胞群BrilliantViolet421平均比PacificBlue™明亮10倍，比PE明亮2-3倍BrilliantViolet™421BrilliantViolet™421应用BrilliantViolet421开展Treg研究BDHorizon™V450Ex-Max404nm/Em-Max448nm.SpectralpropertiessimilartoPacificBlue™.ComparisonoffluorescenceintensitiesofBDHorizonV450andPacificBlue™reagents.BDHorizon™V450StainindexcomparisonbetweenBDHorizonV450andPacificBlue™reagentsacrossseveralantibodyclones.BDHorizon™V500Ex-Max415nm/Em-Max500nm.ImprovedbrightnessoverPacificOrange™andreducedspilloverintotheFITCchannelwhencomparedtoAmCyan.BDHorizonV500isbrighterthanequivalentPacificOrange™conjugates.BDHorizon™V500BDHorizonV500conjugatesofferdramaticallyreducedspilloverintotheadjacentFITCchannelwhencomparedtoAmCyan.BDHorizon™V500ExampledataofBDHorizonV500usedincombinationwithBDHorizon™V450.BDHorizon™V500BrilliantViolet™605Ex-Max407nm/Em-Max602nm.Tandemfluorochrome,BrilliantViolet421andCy™3.5.ExcitedbythevioletlaseranddetectedwithastandardPE-CF594/TexasRed®filterset(eg,610/20-nmfilter).Verybright,similartoequivalentPEconjugates.Secondorthirdcolor

forthevioletlaser.BrilliantViolet™605BrilliantViolet™605

BD流式细胞术培训中心COE（上海，北京）

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BD编撰《FlowCytometry》刊物，不断向客户传达更多、

更新的流式资讯BD——BeWithYou!BD为您提供专业流式服务BDCOE（卓越中心）——前所未有的流式4S体验！