CORM-401-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemECORM-401Cat.No.:HY-109804CASNo.:1001015-18-4分⼦式:C₈H₆MnNO₆S₂分⼦量:331.2作⽤靶点:ReactiveOxygenSpecies作⽤通路:Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB储存⽅式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:25mg/mL(75.48mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.0193mL15.0966mL30.1932mL5mM0.6039mL3.0193mL6.0386mL10mM0.3019mL1.5097mL3.0193mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2.5mg/mL(7.55mM);Suspendedsolution;Needultrasonic2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(7.55mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性CORM-401⼀种对氧化剂敏感的CO释放分⼦。CORM-401诱导内⽪钙信号调节中的NO增加。CORM-401减少TNF-α/CHX和H2O2诱导的ROS产⽣。CORM-401解耦线粒体呼吸并抑制糖酵解[1][2][3]。体外研究CORM-401(100µM;1h)inducestheNOproductioninendothelialEA.hy926cells[1].CORM-401(30µM)inducespeak-likecalciumsignalandaugmentescouplingofendoplasmicreticulumandplasmalemmalstore-operatedcalciumchannels[1].CORM-401(50µM;1h)significantlyreducesTNF-α/CHXandH2O2-inducedROSproductionandcelldeath[2].CORM-401(0.5,1mM)inducesapersistentincreaseintheoxygenconsumptionrateinendothelialEA.hy926cells[3].CORM-401(10,30,100µM)inducesaconcentration-dependentincreaseinOCRandasimultaneousdecreaseinECAR[3].REFERENCES[1].KaczaraP,etal.CORM-401inducescalciumsignalling,NOincreaseandactivationofpentosephosphatepathwayinendothelialcells.FEBSJ.2018Apr;285(7):1346-1358.[2].BabuD,etal.DifferentialEffectsofCORM-2andCORM-401inMurineIntestinalEpithelialMODE-KCellsunderOxidativeStress.FrontPharmacol.2017Feb8;8:31.[3].KaczaraP,etal.CarbonmonoxidereleasedbyCORM-401uncouplesmitochondrialrespirationandinhibitsglycolysisinendothelialcells:AroleformitoBKCachannels.BiochimBiophysActa.2015Oct;1847(10):1297-309.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forre